Patent
Atlas literature
Patent
US 10,316,290Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 (a) is a graph of cell viability of hMSCs grown on different substrates including silicon wafer with 300nm SiO 2 (Si/SiO₂), polyethylene terephthalate WO …
FIG. 2 shows Raman analyses and optical images of(a) graphene on Si/SiO₂ after removal of cells and cleaning with acetone, (b) graphene on Si/Si O₂ after …
FIG. 3 shows i m munostaining of cells growing on Si/SiO2, PDMS and PET without BMP-2 growth factor. Cells are stained with DAPI (blue) and either CD-44, 10 …
FIG. 4 (a) is an optical image of a lx1 cm graphene coated Si/Si O 2, showing the graphene boundary.
FIG. 5 shows a quantitative, functional proof of graphene-mediated hMSCs' differentiation into osteocytes via Alizar in Red assay, (i) in the presence (dark …
FIG. 6 shows time-dependent immunostaining of hMSCs growing on Si/SiO₂ substrates either treated with B MP -2 or coated with graphene. Experiments were 30 …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of directing stem cell differentiation in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, comprising: placing stem cells on a continuous single-layer 2D graphene sheet coated on a substrate and exposing to a culture medium for a period of time sufficient to allow the stem cells to differentiate, in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated c ells. Previously presented
The method of Claim 1, wherein the stem cells are mesenchymal stem cells. Previously presented
The method of Claim 1, wherein the stem cells are progenitor cells. Previously presented
The method of Claim 1, wherein the graphene sheet is in the culture medium. Previously presented
The method of Claim 1, wherein the culture medium is a stem cell medium. Previously presented
The method of Claim 1, wherein the culture medium is an osteogenic medium. Previously presented
The method of Claim 1, wherein the stem cell differentiation is osteogenic differentiation. Previously presented
The method of Claim 1, wherein the differentiated cells are osteoblasts or osteocytes. Previously presented
The method of Claim 1, wherein the substrate is a biocompatible material. Previously presented
- 5. Canceled
Canceled
(Withdrawn-Previously Presented) A method of repairing and improving bone tissue function comprising directing stem cell differentiation by placing stem cells on a continuous single-layer 2D graphene sheet coated on a substrate; allowing the stem cells to differentiate in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated stem cells; and transplanting the differentiated stem cells in the tissue at the site of repair. Withdrawn
(Withdrawn-Previously Presented) The method of Claim 14, where the stem cells are mesenchymal stem cells. Withdrawn
(Withdrawn-Previously Presented) The method of Claim 14, where the culture medium is osteogenic medium. Withdrawn
-20. Canceled
Canceled
(Withdrawn-Previously Presented) A method of directing stem cell differentiation, comprising: coating a continuous single-layer 2D graphene sheet on an implantable, biocompatible scaffold and placing the implantable, biocompatible scaffold at an implantation site to promote differentiation of stem cells in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated cells at the implantation site. Withdrawn
-24. Canceled
Canceled
A method of directing stem cell differentiation into osteogenic cells, comprising: Docket No.: 4459.1028-001 Expedited Procedure under 37 CFR @ 1.116 placing stem cells on a continuous single-layer 2D graphene sheet, produced by chemical vapor deposition, coated on a substrate and exposing to an osteogenic culture medium that is not supplemented with Bone Morpho g enetic Protein-2, for a period of time sufficient to allow the stem cells to differentiate into osteogenic cells. Currently amended
The method of Claim 25, wherein the osteogenic cells are osteoblasts. Original
-28. Canceled
Canceled
Materials described outside the worked examples.
single-layer graphene
C
polydimethylsiloxane (PDMS)
Additional fabrication and treatment steps described in the patent.
Measurements and analyses referenced in the patent, with their drawing references.
FIG. 1 (a) is a graph of cell viability of hMSCs grown on different substrates including silicon wafer with 300nm SiO 2 (Si/SiO₂), polyethylene terephthalate WO …
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Young's modulus of graphene | 0.5–1 | C |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,316,290Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 (a) is a graph of cell viability of hMSCs grown on different substrates including silicon wafer with 300nm SiO 2 (Si/SiO₂), polyethylene terephthalate WO …
FIG. 2 shows Raman analyses and optical images of(a) graphene on Si/SiO₂ after removal of cells and cleaning with acetone, (b) graphene on Si/Si O₂ after …
FIG. 3 shows i m munostaining of cells growing on Si/SiO2, PDMS and PET without BMP-2 growth factor. Cells are stained with DAPI (blue) and either CD-44, 10 …
FIG. 4 (a) is an optical image of a lx1 cm graphene coated Si/Si O 2, showing the graphene boundary.
FIG. 5 shows a quantitative, functional proof of graphene-mediated hMSCs' differentiation into osteocytes via Alizar in Red assay, (i) in the presence (dark …
FIG. 6 shows time-dependent immunostaining of hMSCs growing on Si/SiO₂ substrates either treated with B MP -2 or coated with graphene. Experiments were 30 …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of directing stem cell differentiation in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, comprising: placing stem cells on a continuous single-layer 2D graphene sheet coated on a substrate and exposing to a culture medium for a period of time sufficient to allow the stem cells to differentiate, in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated c ells. Previously presented
The method of Claim 1, wherein the stem cells are mesenchymal stem cells. Previously presented
The method of Claim 1, wherein the stem cells are progenitor cells. Previously presented
The method of Claim 1, wherein the graphene sheet is in the culture medium. Previously presented
The method of Claim 1, wherein the culture medium is a stem cell medium. Previously presented
The method of Claim 1, wherein the culture medium is an osteogenic medium. Previously presented
The method of Claim 1, wherein the stem cell differentiation is osteogenic differentiation. Previously presented
The method of Claim 1, wherein the differentiated cells are osteoblasts or osteocytes. Previously presented
The method of Claim 1, wherein the substrate is a biocompatible material. Previously presented
- 5. Canceled
Canceled
(Withdrawn-Previously Presented) A method of repairing and improving bone tissue function comprising directing stem cell differentiation by placing stem cells on a continuous single-layer 2D graphene sheet coated on a substrate; allowing the stem cells to differentiate in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated stem cells; and transplanting the differentiated stem cells in the tissue at the site of repair. Withdrawn
(Withdrawn-Previously Presented) The method of Claim 14, where the stem cells are mesenchymal stem cells. Withdrawn
(Withdrawn-Previously Presented) The method of Claim 14, where the culture medium is osteogenic medium. Withdrawn
-20. Canceled
Canceled
(Withdrawn-Previously Presented) A method of directing stem cell differentiation, comprising: coating a continuous single-layer 2D graphene sheet on an implantable, biocompatible scaffold and placing the implantable, biocompatible scaffold at an implantation site to promote differentiation of stem cells in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated cells at the implantation site. Withdrawn
-24. Canceled
Canceled
A method of directing stem cell differentiation into osteogenic cells, comprising: Docket No.: 4459.1028-001 Expedited Procedure under 37 CFR @ 1.116 placing stem cells on a continuous single-layer 2D graphene sheet, produced by chemical vapor deposition, coated on a substrate and exposing to an osteogenic culture medium that is not supplemented with Bone Morpho g enetic Protein-2, for a period of time sufficient to allow the stem cells to differentiate into osteogenic cells. Currently amended
The method of Claim 25, wherein the osteogenic cells are osteoblasts. Original
-28. Canceled
Canceled
Materials described outside the worked examples.
single-layer graphene
C
polydimethylsiloxane (PDMS)
Additional fabrication and treatment steps described in the patent.
Measurements and analyses referenced in the patent, with their drawing references.
FIG. 1 (a) is a graph of cell viability of hMSCs grown on different substrates including silicon wafer with 300nm SiO 2 (Si/SiO₂), polyethylene terephthalate WO …
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Young's modulus of graphene | 0.5–1 | C |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,316,290Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 (a) is a graph of cell viability of hMSCs grown on different substrates including silicon wafer with 300nm SiO 2 (Si/SiO₂), polyethylene terephthalate WO …
FIG. 2 shows Raman analyses and optical images of(a) graphene on Si/SiO₂ after removal of cells and cleaning with acetone, (b) graphene on Si/Si O₂ after …
FIG. 3 shows i m munostaining of cells growing on Si/SiO2, PDMS and PET without BMP-2 growth factor. Cells are stained with DAPI (blue) and either CD-44, 10 …
FIG. 4 (a) is an optical image of a lx1 cm graphene coated Si/Si O 2, showing the graphene boundary.
FIG. 5 shows a quantitative, functional proof of graphene-mediated hMSCs' differentiation into osteocytes via Alizar in Red assay, (i) in the presence (dark …
FIG. 6 shows time-dependent immunostaining of hMSCs growing on Si/SiO₂ substrates either treated with B MP -2 or coated with graphene. Experiments were 30 …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of directing stem cell differentiation in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, comprising: placing stem cells on a continuous single-layer 2D graphene sheet coated on a substrate and exposing to a culture medium for a period of time sufficient to allow the stem cells to differentiate, in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated c ells. Previously presented
The method of Claim 1, wherein the stem cells are mesenchymal stem cells. Previously presented
The method of Claim 1, wherein the stem cells are progenitor cells. Previously presented
The method of Claim 1, wherein the graphene sheet is in the culture medium. Previously presented
The method of Claim 1, wherein the culture medium is a stem cell medium. Previously presented
The method of Claim 1, wherein the culture medium is an osteogenic medium. Previously presented
The method of Claim 1, wherein the stem cell differentiation is osteogenic differentiation. Previously presented
The method of Claim 1, wherein the differentiated cells are osteoblasts or osteocytes. Previously presented
The method of Claim 1, wherein the substrate is a biocompatible material. Previously presented
- 5. Canceled
Canceled
(Withdrawn-Previously Presented) A method of repairing and improving bone tissue function comprising directing stem cell differentiation by placing stem cells on a continuous single-layer 2D graphene sheet coated on a substrate; allowing the stem cells to differentiate in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated stem cells; and transplanting the differentiated stem cells in the tissue at the site of repair. Withdrawn
(Withdrawn-Previously Presented) The method of Claim 14, where the stem cells are mesenchymal stem cells. Withdrawn
(Withdrawn-Previously Presented) The method of Claim 14, where the culture medium is osteogenic medium. Withdrawn
-20. Canceled
Canceled
(Withdrawn-Previously Presented) A method of directing stem cell differentiation, comprising: coating a continuous single-layer 2D graphene sheet on an implantable, biocompatible scaffold and placing the implantable, biocompatible scaffold at an implantation site to promote differentiation of stem cells in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated cells at the implantation site. Withdrawn
-24. Canceled
Canceled
A method of directing stem cell differentiation into osteogenic cells, comprising: Docket No.: 4459.1028-001 Expedited Procedure under 37 CFR @ 1.116 placing stem cells on a continuous single-layer 2D graphene sheet, produced by chemical vapor deposition, coated on a substrate and exposing to an osteogenic culture medium that is not supplemented with Bone Morpho g enetic Protein-2, for a period of time sufficient to allow the stem cells to differentiate into osteogenic cells. Currently amended
The method of Claim 25, wherein the osteogenic cells are osteoblasts. Original
-28. Canceled
Canceled
Materials described outside the worked examples.
single-layer graphene
C
polydimethylsiloxane (PDMS)
Additional fabrication and treatment steps described in the patent.
Measurements and analyses referenced in the patent, with their drawing references.
FIG. 1 (a) is a graph of cell viability of hMSCs grown on different substrates including silicon wafer with 300nm SiO 2 (Si/SiO₂), polyethylene terephthalate WO …
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Young's modulus of graphene | 0.5–1 | C |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,316,290Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 (a) is a graph of cell viability of hMSCs grown on different substrates including silicon wafer with 300nm SiO 2 (Si/SiO₂), polyethylene terephthalate WO …
FIG. 2 shows Raman analyses and optical images of(a) graphene on Si/SiO₂ after removal of cells and cleaning with acetone, (b) graphene on Si/Si O₂ after …
FIG. 3 shows i m munostaining of cells growing on Si/SiO2, PDMS and PET without BMP-2 growth factor. Cells are stained with DAPI (blue) and either CD-44, 10 …
FIG. 4 (a) is an optical image of a lx1 cm graphene coated Si/Si O 2, showing the graphene boundary.
FIG. 5 shows a quantitative, functional proof of graphene-mediated hMSCs' differentiation into osteocytes via Alizar in Red assay, (i) in the presence (dark …
FIG. 6 shows time-dependent immunostaining of hMSCs growing on Si/SiO₂ substrates either treated with B MP -2 or coated with graphene. Experiments were 30 …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of directing stem cell differentiation in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, comprising: placing stem cells on a continuous single-layer 2D graphene sheet coated on a substrate and exposing to a culture medium for a period of time sufficient to allow the stem cells to differentiate, in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated c ells. Previously presented
The method of Claim 1, wherein the stem cells are mesenchymal stem cells. Previously presented
The method of Claim 1, wherein the stem cells are progenitor cells. Previously presented
The method of Claim 1, wherein the graphene sheet is in the culture medium. Previously presented
The method of Claim 1, wherein the culture medium is a stem cell medium. Previously presented
The method of Claim 1, wherein the culture medium is an osteogenic medium. Previously presented
The method of Claim 1, wherein the stem cell differentiation is osteogenic differentiation. Previously presented
The method of Claim 1, wherein the differentiated cells are osteoblasts or osteocytes. Previously presented
The method of Claim 1, wherein the substrate is a biocompatible material. Previously presented
- 5. Canceled
Canceled
(Withdrawn-Previously Presented) A method of repairing and improving bone tissue function comprising directing stem cell differentiation by placing stem cells on a continuous single-layer 2D graphene sheet coated on a substrate; allowing the stem cells to differentiate in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated stem cells; and transplanting the differentiated stem cells in the tissue at the site of repair. Withdrawn
(Withdrawn-Previously Presented) The method of Claim 14, where the stem cells are mesenchymal stem cells. Withdrawn
(Withdrawn-Previously Presented) The method of Claim 14, where the culture medium is osteogenic medium. Withdrawn
-20. Canceled
Canceled
(Withdrawn-Previously Presented) A method of directing stem cell differentiation, comprising: coating a continuous single-layer 2D graphene sheet on an implantable, biocompatible scaffold and placing the implantable, biocompatible scaffold at an implantation site to promote differentiation of stem cells in the absence of growth factors and an external stimulant that can stimulate cellular proliferation and differentiation, to produce differentiated cells at the implantation site. Withdrawn
-24. Canceled
Canceled
A method of directing stem cell differentiation into osteogenic cells, comprising: Docket No.: 4459.1028-001 Expedited Procedure under 37 CFR @ 1.116 placing stem cells on a continuous single-layer 2D graphene sheet, produced by chemical vapor deposition, coated on a substrate and exposing to an osteogenic culture medium that is not supplemented with Bone Morpho g enetic Protein-2, for a period of time sufficient to allow the stem cells to differentiate into osteogenic cells. Currently amended
The method of Claim 25, wherein the osteogenic cells are osteoblasts. Original
-28. Canceled
Canceled
Materials described outside the worked examples.
single-layer graphene
C
polydimethylsiloxane (PDMS)
Additional fabrication and treatment steps described in the patent.
Measurements and analyses referenced in the patent, with their drawing references.
FIG. 1 (a) is a graph of cell viability of hMSCs grown on different substrates including silicon wafer with 300nm SiO 2 (Si/SiO₂), polyethylene terephthalate WO …
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Young's modulus of graphene | 0.5–1 | C |
Related documents with shared materials, methods, properties, or citations.
polyethylene terephthalate (PET)
silicon wafer with 300nm SiO₂ (Si/SiO₂)
quartz
SiO₂
FIG. 2 shows Raman analyses and optical images of(a) graphene on Si/SiO₂ after removal of cells and cleaning with acetone, (b) graphene on Si/Si O₂ after …
FIG. 6 shows time-dependent immunostaining of hMSCs growing on Si/SiO₂ substrates either treated with B MP -2 or coated with graphene. Experiments were 30 …
polyethylene terephthalate (PET)
silicon wafer with 300nm SiO₂ (Si/SiO₂)
quartz
SiO₂
FIG. 2 shows Raman analyses and optical images of(a) graphene on Si/SiO₂ after removal of cells and cleaning with acetone, (b) graphene on Si/Si O₂ after …
FIG. 6 shows time-dependent immunostaining of hMSCs growing on Si/SiO₂ substrates either treated with B MP -2 or coated with graphene. Experiments were 30 …
polyethylene terephthalate (PET)
silicon wafer with 300nm SiO₂ (Si/SiO₂)
quartz
SiO₂
FIG. 2 shows Raman analyses and optical images of(a) graphene on Si/SiO₂ after removal of cells and cleaning with acetone, (b) graphene on Si/Si O₂ after …
FIG. 6 shows time-dependent immunostaining of hMSCs growing on Si/SiO₂ substrates either treated with B MP -2 or coated with graphene. Experiments were 30 …
polyethylene terephthalate (PET)
silicon wafer with 300nm SiO₂ (Si/SiO₂)
quartz
SiO₂
FIG. 2 shows Raman analyses and optical images of(a) graphene on Si/SiO₂ after removal of cells and cleaning with acetone, (b) graphene on Si/Si O₂ after …
FIG. 6 shows time-dependent immunostaining of hMSCs growing on Si/SiO₂ substrates either treated with B MP -2 or coated with graphene. Experiments were 30 …
