Patent
US 10,149,862METHOD OF MAKING NANOCOMPOSITES OF METAL OXIDE AND REDUCED GRAPHENE OXIDE AND USE FOR CANCER TREATMENT
Edreese H. Alsharaeh, Faheen Ahmed, Nishat Arshi, Yasmin Mussa et al.
US
Copyright © 2026 Matter42. All rights reserved.
Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
(Withdrawn/Currently Amended) A method to make a C o 3 0 4/rGO nanocomposite to treat a human cancer cell s line, comp ri sing: stirring a solution of cetyl trimethyl ammonium bromide and a graphene oxide solution for 30 minutes; mixing a cobalt acetate solution in water to a stirred solution dropwise to make a solution; adding a reducing agent to the solution; irradiating the solution after adding the reducing agent in a microwave using a specific power to make a product; washing the product several times using a water and an ethanol; drying a washed product at 80 ° C for two hours; calcining and grinding a dried product to obtain the C o 3 0 4/rGO nanocomposite; adding the C o 3 0 4/rGO nanocomposite at various concentrations to a human cancer cell line; and inhibiting the cell proliferation of the human cancer cell line. Currently amended
The method of claim 1, further comprising; adding the C o0O 4/rGO nanocomposite at a concentration between 25-200ug/m l of a cell media to inhibit cell growth of cancer cells. Withdrawn
The method of claim 1, wherein the specific power of the microwave is 100W. Withdrawn
(Withdrawn/Previously Amended) The method of claim 1, wherein the graphene oxide solution has a concentration of 2mg/ml. Currently amended
The method of claim 1, wherein the reducing agent is hydrazine hydrate. Withdrawn
A method of making a black colored metal oxide/reduced graphene oxide nanocomposite to treat a human cancer cell & line, comprising: adding a cationic surfactant dissolved in a distilled water to a graphene oxide solution to make a homogeneous solution; stirring the homogeneous solution and adding a second compound solution dropwise to make a solution, wherein the second compound solution is cobalt acetate solution; stirring the solution and adding a reducing agent to make a processed solution; microwaving the processed solution at a specific power for 20 minutes to obtain a C-e-9 4 metal oxide/reduced graphene oxide (rGO) nanocomposite; and washing the C 444 metal oxide/reduced graphene oxide nanocomposite with water and ethanol and drying at 80 0 C overnight to obtain a dried sample; grinding the dried sample and calcining at 600 0 C for 2 hours to obtain the black colored 4 metal oxide/reduced graphene oxide nanocomposite; adding the black colored metal oxide Ce" 44/rGO nanocomposite at various concentrations to a the human cancer cell line[;] and inhibiting the cell proliferation of the human cancer cell line. Currently amended
(Previously Amended) The method of claim 6, wherein the metal oxide in the black colored metal oxide/reduced graphene oxide nanocomposite is a Co 3 O 4. Currently amended
(Previously Amended) The method of claim 6, wherein the black colored metal oxide/reduced graphene oxide nanocomposite is a Co 3 O 4/rGO nanocomposite. Currently amended
The method of claim 6, wherein the reducing agent is hydrazine hydrate. Currently amended
The method of claim 6, wherein the specific power of the microwave is 100W. Original
(Previously Amended) The method of claim 6, wherein the graphene oxide solution is made at 2mg/m i. Currently amended
(Withdrawn/Currently Amended) A method of making a metal oxide/reduced graphene oxide nanocomposite to treat a human cancer cell line, comprising: adding a ferric chloride and 2g of a ferric sulfate were mixed with 0.4g of a graphene oxide in 50m l deionized water to make a solution; stirring the solution to maintain a homogenous solution; adding 8 ml of 25% ammonia solution was added drop wise under vigorous stirring and 8 ml polyethylene glycol (PEG) was added to the homogenous solution; heating the homogenous solution [[,]] which contains the ammonia and PEG to 80 ° C on a hot plate to make a metal oxide/graphene nanocomposite; washing the metal oxide/graphene nanocomposite by centrifugation at 3000 rpm several times with deionized water and with ethyl alcohol; drying the metal oxide/graphene nanocomposite at 80 ° C in the oven overnight [;] adding the metal oxide/reduced graphene oxide nanocomposite at various concentrations to a human cancer cell line; and inhibiting the cell proliferation of the human cancer cell line. Currently amended
The method of claim 11, wherein the metal oxide/graphene nanocomposite is a Fe 3 O 4/rGO nanocomposite. Withdrawn
Canceled
Embodiments described in the patent, grouped by the materials and process steps they use.
5 materials1 process step
920 mg CTAB mixed with 800 ml distilled water and 8% (2 mg/ml) graphene oxide (Hummer's method) in a 1000 ml beaker. After 30 min continuous stirring, 4.6 g cobalt acetate in 333 ml distilled water added dropwise. Then 1 ml hydrazine hydrate added. Solution heated under microwave irradiation for 20 min at 100W. Product washed with distilled water and ethanol, dried at 80°C overnight, ground and calcined at 600°C for 2h. A black coloured product obtained.
6 materials1 process step
2g ferric chloride and 2g ferric sulfate mixed with 0.4g (8%) graphene oxide (Hummer's method) in 50 ml deionized water, stirred for homogeneous solution. 8 ml of 25% ammonia solution added dropwise under vigorous stirring; 8 ml PEG added quickly. Solution heated to 80°C on hot plate. Washed by centrifugation at 3000 rpm with deionized water and 70% ethyl alcohol; dried at 80°C overnight.
2 materials
HepG₂ (liver) human cancer cells grown in RPMI media with 10% bovine serum and 1X penicillin-streptomycin at 37°C, 5% CO2. Cells seeded at 10⁵ cells/well in a 96-well plate. Stocks (1.0 mg/ml) in 10% DMSO; working solutions (100 µg/ml) in serum-free media. Cells treated with 25, 50, 100, and 200 µg/ml doses in triplicate for 48 hours. Cell proliferation/viability assessed by TACS MTT assay. IC₅₀ values calculated.
Materials described outside the worked examples.
cobalt acetate
(CH₃COO)2Co·4H₂O
Measurements and analyses referenced in the patent, with their drawing references.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
(001) diffraction peak of GO | 11.22 deg | GO |
(002) peak of graphite, disappeared after oxidation | 26.5 deg | GO |
Thickness | 200–350 nm | — |
Thickness | 400–580 nm | — |
Thickness | 10–30 nm | — |
Related documents with shared materials, methods, properties, or citations.
Interconnected Reduced Graphene Oxide
Graphene Oxide/Metal Nanocrystal Multilaminates the Atomic Limit for Safe, Selective Hydrogen Storage
THERMOPLASTIC POLYURETHANE GRAPHENE OXIDE NANOCOMPOSITES WITH ENHANCED MECHANICAL BEHAVIOR
A BIOCOMPOSITE OF BIOMINERALIZED GRAPHENE OXIDE AND ITS USE FOR BONE TISSUE ENGINEERING
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SIMPLE PRODUCTION METHOD FOR GRAPHENE BY MICROORGANISMS
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Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
(Withdrawn/Currently Amended) A method to make a C o 3 0 4/rGO nanocomposite to treat a human cancer cell s line, comp ri sing: stirring a solution of cetyl trimethyl ammonium bromide and a graphene oxide solution for 30 minutes; mixing a cobalt acetate solution in water to a stirred solution dropwise to make a solution; adding a reducing agent to the solution; irradiating the solution after adding the reducing agent in a microwave using a specific power to make a product; washing the product several times using a water and an ethanol; drying a washed product at 80 ° C for two hours; calcining and grinding a dried product to obtain the C o 3 0 4/rGO nanocomposite; adding the C o 3 0 4/rGO nanocomposite at various concentrations to a human cancer cell line; and inhibiting the cell proliferation of the human cancer cell line. Currently amended
The method of claim 1, further comprising; adding the C o0O 4/rGO nanocomposite at a concentration between 25-200ug/m l of a cell media to inhibit cell growth of cancer cells. Withdrawn
The method of claim 1, wherein the specific power of the microwave is 100W. Withdrawn
(Withdrawn/Previously Amended) The method of claim 1, wherein the graphene oxide solution has a concentration of 2mg/ml. Currently amended
The method of claim 1, wherein the reducing agent is hydrazine hydrate. Withdrawn
A method of making a black colored metal oxide/reduced graphene oxide nanocomposite to treat a human cancer cell & line, comprising: adding a cationic surfactant dissolved in a distilled water to a graphene oxide solution to make a homogeneous solution; stirring the homogeneous solution and adding a second compound solution dropwise to make a solution, wherein the second compound solution is cobalt acetate solution; stirring the solution and adding a reducing agent to make a processed solution; microwaving the processed solution at a specific power for 20 minutes to obtain a C-e-9 4 metal oxide/reduced graphene oxide (rGO) nanocomposite; and washing the C 444 metal oxide/reduced graphene oxide nanocomposite with water and ethanol and drying at 80 0 C overnight to obtain a dried sample; grinding the dried sample and calcining at 600 0 C for 2 hours to obtain the black colored 4 metal oxide/reduced graphene oxide nanocomposite; adding the black colored metal oxide Ce" 44/rGO nanocomposite at various concentrations to a the human cancer cell line[;] and inhibiting the cell proliferation of the human cancer cell line. Currently amended
(Previously Amended) The method of claim 6, wherein the metal oxide in the black colored metal oxide/reduced graphene oxide nanocomposite is a Co 3 O 4. Currently amended
(Previously Amended) The method of claim 6, wherein the black colored metal oxide/reduced graphene oxide nanocomposite is a Co 3 O 4/rGO nanocomposite. Currently amended
The method of claim 6, wherein the reducing agent is hydrazine hydrate. Currently amended
The method of claim 6, wherein the specific power of the microwave is 100W. Original
(Previously Amended) The method of claim 6, wherein the graphene oxide solution is made at 2mg/m i. Currently amended
(Withdrawn/Currently Amended) A method of making a metal oxide/reduced graphene oxide nanocomposite to treat a human cancer cell line, comprising: adding a ferric chloride and 2g of a ferric sulfate were mixed with 0.4g of a graphene oxide in 50m l deionized water to make a solution; stirring the solution to maintain a homogenous solution; adding 8 ml of 25% ammonia solution was added drop wise under vigorous stirring and 8 ml polyethylene glycol (PEG) was added to the homogenous solution; heating the homogenous solution [[,]] which contains the ammonia and PEG to 80 ° C on a hot plate to make a metal oxide/graphene nanocomposite; washing the metal oxide/graphene nanocomposite by centrifugation at 3000 rpm several times with deionized water and with ethyl alcohol; drying the metal oxide/graphene nanocomposite at 80 ° C in the oven overnight [;] adding the metal oxide/reduced graphene oxide nanocomposite at various concentrations to a human cancer cell line; and inhibiting the cell proliferation of the human cancer cell line. Currently amended
The method of claim 11, wherein the metal oxide/graphene nanocomposite is a Fe 3 O 4/rGO nanocomposite. Withdrawn
Canceled
Embodiments described in the patent, grouped by the materials and process steps they use.
5 materials1 process step
920 mg CTAB mixed with 800 ml distilled water and 8% (2 mg/ml) graphene oxide (Hummer's method) in a 1000 ml beaker. After 30 min continuous stirring, 4.6 g cobalt acetate in 333 ml distilled water added dropwise. Then 1 ml hydrazine hydrate added. Solution heated under microwave irradiation for 20 min at 100W. Product washed with distilled water and ethanol, dried at 80°C overnight, ground and calcined at 600°C for 2h. A black coloured product obtained.
6 materials1 process step
2g ferric chloride and 2g ferric sulfate mixed with 0.4g (8%) graphene oxide (Hummer's method) in 50 ml deionized water, stirred for homogeneous solution. 8 ml of 25% ammonia solution added dropwise under vigorous stirring; 8 ml PEG added quickly. Solution heated to 80°C on hot plate. Washed by centrifugation at 3000 rpm with deionized water and 70% ethyl alcohol; dried at 80°C overnight.
2 materials
HepG₂ (liver) human cancer cells grown in RPMI media with 10% bovine serum and 1X penicillin-streptomycin at 37°C, 5% CO2. Cells seeded at 10⁵ cells/well in a 96-well plate. Stocks (1.0 mg/ml) in 10% DMSO; working solutions (100 µg/ml) in serum-free media. Cells treated with 25, 50, 100, and 200 µg/ml doses in triplicate for 48 hours. Cell proliferation/viability assessed by TACS MTT assay. IC₅₀ values calculated.
Materials described outside the worked examples.
cobalt acetate
(CH₃COO)2Co·4H₂O
Measurements and analyses referenced in the patent, with their drawing references.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
(001) diffraction peak of GO | 11.22 deg | GO |
(002) peak of graphite, disappeared after oxidation | 26.5 deg | GO |
Thickness | 200–350 nm | — |
Thickness | 400–580 nm | — |
Thickness | 10–30 nm | — |
Related documents with shared materials, methods, properties, or citations.
Interconnected Reduced Graphene Oxide
Graphene Oxide/Metal Nanocrystal Multilaminates the Atomic Limit for Safe, Selective Hydrogen Storage
THERMOPLASTIC POLYURETHANE GRAPHENE OXIDE NANOCOMPOSITES WITH ENHANCED MECHANICAL BEHAVIOR
A BIOCOMPOSITE OF BIOMINERALIZED GRAPHENE OXIDE AND ITS USE FOR BONE TISSUE ENGINEERING
Method for fabricating a semicrystalline polymer / graphene oxide composite film
SIMPLE PRODUCTION METHOD FOR GRAPHENE BY MICROORGANISMS
Integral 3D graphene-carbon hybrid foam
Highly conductive graphene foams and process for producing same
Process for Synthesizing Reduced Graphene Oxide on a Substrate from Seedlac
Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
(Withdrawn/Currently Amended) A method to make a C o 3 0 4/rGO nanocomposite to treat a human cancer cell s line, comp ri sing: stirring a solution of cetyl trimethyl ammonium bromide and a graphene oxide solution for 30 minutes; mixing a cobalt acetate solution in water to a stirred solution dropwise to make a solution; adding a reducing agent to the solution; irradiating the solution after adding the reducing agent in a microwave using a specific power to make a product; washing the product several times using a water and an ethanol; drying a washed product at 80 ° C for two hours; calcining and grinding a dried product to obtain the C o 3 0 4/rGO nanocomposite; adding the C o 3 0 4/rGO nanocomposite at various concentrations to a human cancer cell line; and inhibiting the cell proliferation of the human cancer cell line. Currently amended
The method of claim 1, further comprising; adding the C o0O 4/rGO nanocomposite at a concentration between 25-200ug/m l of a cell media to inhibit cell growth of cancer cells. Withdrawn
The method of claim 1, wherein the specific power of the microwave is 100W. Withdrawn
(Withdrawn/Previously Amended) The method of claim 1, wherein the graphene oxide solution has a concentration of 2mg/ml. Currently amended
The method of claim 1, wherein the reducing agent is hydrazine hydrate. Withdrawn
A method of making a black colored metal oxide/reduced graphene oxide nanocomposite to treat a human cancer cell & line, comprising: adding a cationic surfactant dissolved in a distilled water to a graphene oxide solution to make a homogeneous solution; stirring the homogeneous solution and adding a second compound solution dropwise to make a solution, wherein the second compound solution is cobalt acetate solution; stirring the solution and adding a reducing agent to make a processed solution; microwaving the processed solution at a specific power for 20 minutes to obtain a C-e-9 4 metal oxide/reduced graphene oxide (rGO) nanocomposite; and washing the C 444 metal oxide/reduced graphene oxide nanocomposite with water and ethanol and drying at 80 0 C overnight to obtain a dried sample; grinding the dried sample and calcining at 600 0 C for 2 hours to obtain the black colored 4 metal oxide/reduced graphene oxide nanocomposite; adding the black colored metal oxide Ce" 44/rGO nanocomposite at various concentrations to a the human cancer cell line[;] and inhibiting the cell proliferation of the human cancer cell line. Currently amended
(Previously Amended) The method of claim 6, wherein the metal oxide in the black colored metal oxide/reduced graphene oxide nanocomposite is a Co 3 O 4. Currently amended
(Previously Amended) The method of claim 6, wherein the black colored metal oxide/reduced graphene oxide nanocomposite is a Co 3 O 4/rGO nanocomposite. Currently amended
The method of claim 6, wherein the reducing agent is hydrazine hydrate. Currently amended
The method of claim 6, wherein the specific power of the microwave is 100W. Original
(Previously Amended) The method of claim 6, wherein the graphene oxide solution is made at 2mg/m i. Currently amended
(Withdrawn/Currently Amended) A method of making a metal oxide/reduced graphene oxide nanocomposite to treat a human cancer cell line, comprising: adding a ferric chloride and 2g of a ferric sulfate were mixed with 0.4g of a graphene oxide in 50m l deionized water to make a solution; stirring the solution to maintain a homogenous solution; adding 8 ml of 25% ammonia solution was added drop wise under vigorous stirring and 8 ml polyethylene glycol (PEG) was added to the homogenous solution; heating the homogenous solution [[,]] which contains the ammonia and PEG to 80 ° C on a hot plate to make a metal oxide/graphene nanocomposite; washing the metal oxide/graphene nanocomposite by centrifugation at 3000 rpm several times with deionized water and with ethyl alcohol; drying the metal oxide/graphene nanocomposite at 80 ° C in the oven overnight [;] adding the metal oxide/reduced graphene oxide nanocomposite at various concentrations to a human cancer cell line; and inhibiting the cell proliferation of the human cancer cell line. Currently amended
The method of claim 11, wherein the metal oxide/graphene nanocomposite is a Fe 3 O 4/rGO nanocomposite. Withdrawn
Canceled
Embodiments described in the patent, grouped by the materials and process steps they use.
5 materials1 process step
920 mg CTAB mixed with 800 ml distilled water and 8% (2 mg/ml) graphene oxide (Hummer's method) in a 1000 ml beaker. After 30 min continuous stirring, 4.6 g cobalt acetate in 333 ml distilled water added dropwise. Then 1 ml hydrazine hydrate added. Solution heated under microwave irradiation for 20 min at 100W. Product washed with distilled water and ethanol, dried at 80°C overnight, ground and calcined at 600°C for 2h. A black coloured product obtained.
6 materials1 process step
2g ferric chloride and 2g ferric sulfate mixed with 0.4g (8%) graphene oxide (Hummer's method) in 50 ml deionized water, stirred for homogeneous solution. 8 ml of 25% ammonia solution added dropwise under vigorous stirring; 8 ml PEG added quickly. Solution heated to 80°C on hot plate. Washed by centrifugation at 3000 rpm with deionized water and 70% ethyl alcohol; dried at 80°C overnight.
2 materials
HepG₂ (liver) human cancer cells grown in RPMI media with 10% bovine serum and 1X penicillin-streptomycin at 37°C, 5% CO2. Cells seeded at 10⁵ cells/well in a 96-well plate. Stocks (1.0 mg/ml) in 10% DMSO; working solutions (100 µg/ml) in serum-free media. Cells treated with 25, 50, 100, and 200 µg/ml doses in triplicate for 48 hours. Cell proliferation/viability assessed by TACS MTT assay. IC₅₀ values calculated.
Materials described outside the worked examples.
cobalt acetate
(CH₃COO)2Co·4H₂O
Measurements and analyses referenced in the patent, with their drawing references.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
(001) diffraction peak of GO | 11.22 deg | GO |
(002) peak of graphite, disappeared after oxidation | 26.5 deg | GO |
Thickness | 200–350 nm | — |
Thickness | 400–580 nm | — |
Thickness | 10–30 nm | — |
Related documents with shared materials, methods, properties, or citations.
Interconnected Reduced Graphene Oxide
Graphene Oxide/Metal Nanocrystal Multilaminates the Atomic Limit for Safe, Selective Hydrogen Storage
THERMOPLASTIC POLYURETHANE GRAPHENE OXIDE NANOCOMPOSITES WITH ENHANCED MECHANICAL BEHAVIOR
A BIOCOMPOSITE OF BIOMINERALIZED GRAPHENE OXIDE AND ITS USE FOR BONE TISSUE ENGINEERING
Method for fabricating a semicrystalline polymer / graphene oxide composite film
SIMPLE PRODUCTION METHOD FOR GRAPHENE BY MICROORGANISMS
Integral 3D graphene-carbon hybrid foam
Highly conductive graphene foams and process for producing same
Process for Synthesizing Reduced Graphene Oxide on a Substrate from Seedlac
Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
(Withdrawn/Currently Amended) A method to make a C o 3 0 4/rGO nanocomposite to treat a human cancer cell s line, comp ri sing: stirring a solution of cetyl trimethyl ammonium bromide and a graphene oxide solution for 30 minutes; mixing a cobalt acetate solution in water to a stirred solution dropwise to make a solution; adding a reducing agent to the solution; irradiating the solution after adding the reducing agent in a microwave using a specific power to make a product; washing the product several times using a water and an ethanol; drying a washed product at 80 ° C for two hours; calcining and grinding a dried product to obtain the C o 3 0 4/rGO nanocomposite; adding the C o 3 0 4/rGO nanocomposite at various concentrations to a human cancer cell line; and inhibiting the cell proliferation of the human cancer cell line. Currently amended
The method of claim 1, further comprising; adding the C o0O 4/rGO nanocomposite at a concentration between 25-200ug/m l of a cell media to inhibit cell growth of cancer cells. Withdrawn
The method of claim 1, wherein the specific power of the microwave is 100W. Withdrawn
(Withdrawn/Previously Amended) The method of claim 1, wherein the graphene oxide solution has a concentration of 2mg/ml. Currently amended
The method of claim 1, wherein the reducing agent is hydrazine hydrate. Withdrawn
A method of making a black colored metal oxide/reduced graphene oxide nanocomposite to treat a human cancer cell & line, comprising: adding a cationic surfactant dissolved in a distilled water to a graphene oxide solution to make a homogeneous solution; stirring the homogeneous solution and adding a second compound solution dropwise to make a solution, wherein the second compound solution is cobalt acetate solution; stirring the solution and adding a reducing agent to make a processed solution; microwaving the processed solution at a specific power for 20 minutes to obtain a C-e-9 4 metal oxide/reduced graphene oxide (rGO) nanocomposite; and washing the C 444 metal oxide/reduced graphene oxide nanocomposite with water and ethanol and drying at 80 0 C overnight to obtain a dried sample; grinding the dried sample and calcining at 600 0 C for 2 hours to obtain the black colored 4 metal oxide/reduced graphene oxide nanocomposite; adding the black colored metal oxide Ce" 44/rGO nanocomposite at various concentrations to a the human cancer cell line[;] and inhibiting the cell proliferation of the human cancer cell line. Currently amended
(Previously Amended) The method of claim 6, wherein the metal oxide in the black colored metal oxide/reduced graphene oxide nanocomposite is a Co 3 O 4. Currently amended
(Previously Amended) The method of claim 6, wherein the black colored metal oxide/reduced graphene oxide nanocomposite is a Co 3 O 4/rGO nanocomposite. Currently amended
The method of claim 6, wherein the reducing agent is hydrazine hydrate. Currently amended
The method of claim 6, wherein the specific power of the microwave is 100W. Original
(Previously Amended) The method of claim 6, wherein the graphene oxide solution is made at 2mg/m i. Currently amended
(Withdrawn/Currently Amended) A method of making a metal oxide/reduced graphene oxide nanocomposite to treat a human cancer cell line, comprising: adding a ferric chloride and 2g of a ferric sulfate were mixed with 0.4g of a graphene oxide in 50m l deionized water to make a solution; stirring the solution to maintain a homogenous solution; adding 8 ml of 25% ammonia solution was added drop wise under vigorous stirring and 8 ml polyethylene glycol (PEG) was added to the homogenous solution; heating the homogenous solution [[,]] which contains the ammonia and PEG to 80 ° C on a hot plate to make a metal oxide/graphene nanocomposite; washing the metal oxide/graphene nanocomposite by centrifugation at 3000 rpm several times with deionized water and with ethyl alcohol; drying the metal oxide/graphene nanocomposite at 80 ° C in the oven overnight [;] adding the metal oxide/reduced graphene oxide nanocomposite at various concentrations to a human cancer cell line; and inhibiting the cell proliferation of the human cancer cell line. Currently amended
The method of claim 11, wherein the metal oxide/graphene nanocomposite is a Fe 3 O 4/rGO nanocomposite. Withdrawn
Canceled
Embodiments described in the patent, grouped by the materials and process steps they use.
5 materials1 process step
920 mg CTAB mixed with 800 ml distilled water and 8% (2 mg/ml) graphene oxide (Hummer's method) in a 1000 ml beaker. After 30 min continuous stirring, 4.6 g cobalt acetate in 333 ml distilled water added dropwise. Then 1 ml hydrazine hydrate added. Solution heated under microwave irradiation for 20 min at 100W. Product washed with distilled water and ethanol, dried at 80°C overnight, ground and calcined at 600°C for 2h. A black coloured product obtained.
6 materials1 process step
2g ferric chloride and 2g ferric sulfate mixed with 0.4g (8%) graphene oxide (Hummer's method) in 50 ml deionized water, stirred for homogeneous solution. 8 ml of 25% ammonia solution added dropwise under vigorous stirring; 8 ml PEG added quickly. Solution heated to 80°C on hot plate. Washed by centrifugation at 3000 rpm with deionized water and 70% ethyl alcohol; dried at 80°C overnight.
2 materials
HepG₂ (liver) human cancer cells grown in RPMI media with 10% bovine serum and 1X penicillin-streptomycin at 37°C, 5% CO2. Cells seeded at 10⁵ cells/well in a 96-well plate. Stocks (1.0 mg/ml) in 10% DMSO; working solutions (100 µg/ml) in serum-free media. Cells treated with 25, 50, 100, and 200 µg/ml doses in triplicate for 48 hours. Cell proliferation/viability assessed by TACS MTT assay. IC₅₀ values calculated.
Materials described outside the worked examples.
cobalt acetate
(CH₃COO)2Co·4H₂O
Measurements and analyses referenced in the patent, with their drawing references.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
(001) diffraction peak of GO | 11.22 deg | GO |
(002) peak of graphite, disappeared after oxidation | 26.5 deg | GO |
Thickness | 200–350 nm | — |
Thickness | 400–580 nm | — |
Thickness | 10–30 nm | — |
Related documents with shared materials, methods, properties, or citations.
Interconnected Reduced Graphene Oxide
Graphene Oxide/Metal Nanocrystal Multilaminates the Atomic Limit for Safe, Selective Hydrogen Storage
THERMOPLASTIC POLYURETHANE GRAPHENE OXIDE NANOCOMPOSITES WITH ENHANCED MECHANICAL BEHAVIOR
A BIOCOMPOSITE OF BIOMINERALIZED GRAPHENE OXIDE AND ITS USE FOR BONE TISSUE ENGINEERING
Method for fabricating a semicrystalline polymer / graphene oxide composite film
SIMPLE PRODUCTION METHOD FOR GRAPHENE BY MICROORGANISMS
Integral 3D graphene-carbon hybrid foam
Highly conductive graphene foams and process for producing same
Process for Synthesizing Reduced Graphene Oxide on a Substrate from Seedlac