Patent
US 10,598,658REDUCED GRAPHENE OXIDE-BASED BIOSENSOR AND USE THEREOF
Mu-Yi Hua, Hsiao-Chien Chen, Yi-Ting Chen, Rung-Ywan Tsai et al.
US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 10,598,658Patent drawings and their descriptions. Click a drawing to enlarge it.
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Figure 2 is a photo illustrating a comparison of the solubilities of graphite, a modified graphene product (GLA 1 3) of Preparation Example 1, and a modified graphene product (GSA) of Comparative Preparation Example 1 in water;
Figure 3 is a plot illustrating absorption spectra of graphite, modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and a modified 20 graphene product (GSA) of Comparative Preparation Example 1
Figure 4 is a plot illustrating an X-ray photoelectron spectrum of graphite;
Figure 5 is a plot illustrating an X-ray photoelectron spectrum of the modified graphene product (GLA 1 3) of Preparation Example 2
Figure 6 is a plot illustrating Fourier transform infrared (FT-IR) spectra of 25 graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 7 is a bar chart illustrating a comparison of the hydroxyl group densities of graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation 4 PATENTS 218091-0014 PE-51941-AM Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 8 is a plot illustrating thermogravimetric analysis spectra of graphite, the modified graphene product (GLA 1 3) of Preparation Example 2, and the modified 0 graphene product (GSA) of Comparative Preparation Example 1
Figure 9 is a plot illustrating X-ray diffraction (XRD) spectra of Ni, graphite, and the modified graphene products (GLA 1 1, GLA 13, and GLA 16) of Preparation Examples 1 -3;
Figure 10 is a transmission electron microscopic (TEM) image illustrating a 10 structure of the modified graphene product (GLA 1 1) of Preparation Example 1
Figure 11 is a TEM image illustrating a structure of the modified graphene product (GLA₁₃) of Preparation Example 2
Figure 12 is a TEM image illustrating a structure of the modified graphene product (GLA₁₆) of Preparation Example 3 0
Figure 13 is a TEM image illustrating a structure of the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 14 is a plot illustrating absorption spectra of iron oxide (Fe 3 0 4), the modified graphene product (GLA 1 3) of Preparation Example 2, and the magnetizied modified graphene product (MGLA) of Preparation Example 4 20
Figure 15 is a photo illustrating magnetic analysis results for the modified graphene product (GLA₁₃) of Preparation Example 2 and the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 16 is a plot illustrating XRD spectra of the modified graphene product (GLA 1 3) of Preparation Example 2 and the magnetized modified graphene product 25 (MGLA) of Preparation
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A reduced graphene oxide-based biosensor comprising a na no -structure field-effect transistor, said transistor including a channel region that contains a composite formed [from] of (i) a reduced graphene oxide having a linking moiety of the formula -(C =O)-X- C OOH, X being a C 2 -C₃ alkenylene group or a C₁ -C 3 alkylene group, bonded to an antibody specific to an analyte, a-C 4 -C3; and (ii) a magnetic nanoparticle adsorbed onto said reduced graphene oxide. Currently amended
The reduced graphene oxide-based biosensor according to Claim 1, wherein said channel region further contains an immobilization layer for immobilizing said composite. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein X is a C 2 -C 3 alkenylene group. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said nanostructure field-effect transistor is a polycrystalline silicon nanowire field-effect transistor. Original
The reduced graphene oxide-based biosensor according to Claim 1, wherein said magnetic nanoparticle is selected from a group consisting of a Fe₃ 0 4 magnetic nanoparticle, a Ni magnetic nanoparticle, and a combination thereof. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said composite has a size ranging from 30 nm to 50 nm. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said reduced graphene oxide having said linking moiety is made by subjecting a cyclic dianhydride compound and a graphite to a Friedel-Crafts reaction in the presence of a Lewis acid. Original
A method for detecting an analyte using the according to Claim 1, comprising the steps of: region through a gate electrode of the reduced reduced graphene oxide-based biosensor into contact current of the reduced graphene oxide-based oxide-based biosensor is brought with contact with the analyte. Withdrawn reduced graphene oxide-based biosensor applying a predetermined potential to a channel graphene oxide-based biosensor; bringing the with the analyte; and measuring a change in biosensor before and after the reduced graphene the analyte for determining a concentration of
. Canceled
. Canceled
The reduced graphene oxide-based biosensor according to Claim [[4]] 1, wherein said antibody is an anti-APOA₂ antibody. Currently amended
Embodiments described in the patent, grouped by the materials and process steps they use.
3 materials1 process step
Preparation of reduced graphene oxide (GLA11, GLA13, GLA₁₆) having a linking moiety of -(C=O)-X-COOH using AlCl₃ (Lewis acid) and maleic anhydride (MA) via Friedel-Crafts reaction on graphite.
Layer stacks claimed or described, ordered top of device to substrate.
reduced graphene oxide-based biosensor with nanostructure field-effect transistor
polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET)
Materials described outside the worked examples.
antibody specific to analyte
composite of reduced graphene oxide and magnetic nanoparticle
anti-APOA₂ antibody
Measurements and analyses referenced in the patent, with their drawing references.
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
composite size range | 30–50 nm | composite of reduced graphene oxide and magnetic nanoparticle |
magnetic nanoparticle average particle diameter | 4–50 nm |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,598,658Patent drawings and their descriptions. Click a drawing to enlarge it.
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Figure 2 is a photo illustrating a comparison of the solubilities of graphite, a modified graphene product (GLA 1 3) of Preparation Example 1, and a modified graphene product (GSA) of Comparative Preparation Example 1 in water;
Figure 3 is a plot illustrating absorption spectra of graphite, modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and a modified 20 graphene product (GSA) of Comparative Preparation Example 1
Figure 4 is a plot illustrating an X-ray photoelectron spectrum of graphite;
Figure 5 is a plot illustrating an X-ray photoelectron spectrum of the modified graphene product (GLA 1 3) of Preparation Example 2
Figure 6 is a plot illustrating Fourier transform infrared (FT-IR) spectra of 25 graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 7 is a bar chart illustrating a comparison of the hydroxyl group densities of graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation 4 PATENTS 218091-0014 PE-51941-AM Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 8 is a plot illustrating thermogravimetric analysis spectra of graphite, the modified graphene product (GLA 1 3) of Preparation Example 2, and the modified 0 graphene product (GSA) of Comparative Preparation Example 1
Figure 9 is a plot illustrating X-ray diffraction (XRD) spectra of Ni, graphite, and the modified graphene products (GLA 1 1, GLA 13, and GLA 16) of Preparation Examples 1 -3;
Figure 10 is a transmission electron microscopic (TEM) image illustrating a 10 structure of the modified graphene product (GLA 1 1) of Preparation Example 1
Figure 11 is a TEM image illustrating a structure of the modified graphene product (GLA₁₃) of Preparation Example 2
Figure 12 is a TEM image illustrating a structure of the modified graphene product (GLA₁₆) of Preparation Example 3 0
Figure 13 is a TEM image illustrating a structure of the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 14 is a plot illustrating absorption spectra of iron oxide (Fe 3 0 4), the modified graphene product (GLA 1 3) of Preparation Example 2, and the magnetizied modified graphene product (MGLA) of Preparation Example 4 20
Figure 15 is a photo illustrating magnetic analysis results for the modified graphene product (GLA₁₃) of Preparation Example 2 and the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 16 is a plot illustrating XRD spectra of the modified graphene product (GLA 1 3) of Preparation Example 2 and the magnetized modified graphene product 25 (MGLA) of Preparation
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A reduced graphene oxide-based biosensor comprising a na no -structure field-effect transistor, said transistor including a channel region that contains a composite formed [from] of (i) a reduced graphene oxide having a linking moiety of the formula -(C =O)-X- C OOH, X being a C 2 -C₃ alkenylene group or a C₁ -C 3 alkylene group, bonded to an antibody specific to an analyte, a-C 4 -C3; and (ii) a magnetic nanoparticle adsorbed onto said reduced graphene oxide. Currently amended
The reduced graphene oxide-based biosensor according to Claim 1, wherein said channel region further contains an immobilization layer for immobilizing said composite. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein X is a C 2 -C 3 alkenylene group. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said nanostructure field-effect transistor is a polycrystalline silicon nanowire field-effect transistor. Original
The reduced graphene oxide-based biosensor according to Claim 1, wherein said magnetic nanoparticle is selected from a group consisting of a Fe₃ 0 4 magnetic nanoparticle, a Ni magnetic nanoparticle, and a combination thereof. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said composite has a size ranging from 30 nm to 50 nm. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said reduced graphene oxide having said linking moiety is made by subjecting a cyclic dianhydride compound and a graphite to a Friedel-Crafts reaction in the presence of a Lewis acid. Original
A method for detecting an analyte using the according to Claim 1, comprising the steps of: region through a gate electrode of the reduced reduced graphene oxide-based biosensor into contact current of the reduced graphene oxide-based oxide-based biosensor is brought with contact with the analyte. Withdrawn reduced graphene oxide-based biosensor applying a predetermined potential to a channel graphene oxide-based biosensor; bringing the with the analyte; and measuring a change in biosensor before and after the reduced graphene the analyte for determining a concentration of
. Canceled
. Canceled
The reduced graphene oxide-based biosensor according to Claim [[4]] 1, wherein said antibody is an anti-APOA₂ antibody. Currently amended
Embodiments described in the patent, grouped by the materials and process steps they use.
3 materials1 process step
Preparation of reduced graphene oxide (GLA11, GLA13, GLA₁₆) having a linking moiety of -(C=O)-X-COOH using AlCl₃ (Lewis acid) and maleic anhydride (MA) via Friedel-Crafts reaction on graphite.
Layer stacks claimed or described, ordered top of device to substrate.
reduced graphene oxide-based biosensor with nanostructure field-effect transistor
polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET)
Materials described outside the worked examples.
antibody specific to analyte
composite of reduced graphene oxide and magnetic nanoparticle
anti-APOA₂ antibody
Measurements and analyses referenced in the patent, with their drawing references.
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
composite size range | 30–50 nm | composite of reduced graphene oxide and magnetic nanoparticle |
magnetic nanoparticle average particle diameter | 4–50 nm |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,598,658Patent drawings and their descriptions. Click a drawing to enlarge it.
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Figure 2 is a photo illustrating a comparison of the solubilities of graphite, a modified graphene product (GLA 1 3) of Preparation Example 1, and a modified graphene product (GSA) of Comparative Preparation Example 1 in water;
Figure 3 is a plot illustrating absorption spectra of graphite, modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and a modified 20 graphene product (GSA) of Comparative Preparation Example 1
Figure 4 is a plot illustrating an X-ray photoelectron spectrum of graphite;
Figure 5 is a plot illustrating an X-ray photoelectron spectrum of the modified graphene product (GLA 1 3) of Preparation Example 2
Figure 6 is a plot illustrating Fourier transform infrared (FT-IR) spectra of 25 graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 7 is a bar chart illustrating a comparison of the hydroxyl group densities of graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation 4 PATENTS 218091-0014 PE-51941-AM Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 8 is a plot illustrating thermogravimetric analysis spectra of graphite, the modified graphene product (GLA 1 3) of Preparation Example 2, and the modified 0 graphene product (GSA) of Comparative Preparation Example 1
Figure 9 is a plot illustrating X-ray diffraction (XRD) spectra of Ni, graphite, and the modified graphene products (GLA 1 1, GLA 13, and GLA 16) of Preparation Examples 1 -3;
Figure 10 is a transmission electron microscopic (TEM) image illustrating a 10 structure of the modified graphene product (GLA 1 1) of Preparation Example 1
Figure 11 is a TEM image illustrating a structure of the modified graphene product (GLA₁₃) of Preparation Example 2
Figure 12 is a TEM image illustrating a structure of the modified graphene product (GLA₁₆) of Preparation Example 3 0
Figure 13 is a TEM image illustrating a structure of the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 14 is a plot illustrating absorption spectra of iron oxide (Fe 3 0 4), the modified graphene product (GLA 1 3) of Preparation Example 2, and the magnetizied modified graphene product (MGLA) of Preparation Example 4 20
Figure 15 is a photo illustrating magnetic analysis results for the modified graphene product (GLA₁₃) of Preparation Example 2 and the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 16 is a plot illustrating XRD spectra of the modified graphene product (GLA 1 3) of Preparation Example 2 and the magnetized modified graphene product 25 (MGLA) of Preparation
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A reduced graphene oxide-based biosensor comprising a na no -structure field-effect transistor, said transistor including a channel region that contains a composite formed [from] of (i) a reduced graphene oxide having a linking moiety of the formula -(C =O)-X- C OOH, X being a C 2 -C₃ alkenylene group or a C₁ -C 3 alkylene group, bonded to an antibody specific to an analyte, a-C 4 -C3; and (ii) a magnetic nanoparticle adsorbed onto said reduced graphene oxide. Currently amended
The reduced graphene oxide-based biosensor according to Claim 1, wherein said channel region further contains an immobilization layer for immobilizing said composite. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein X is a C 2 -C 3 alkenylene group. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said nanostructure field-effect transistor is a polycrystalline silicon nanowire field-effect transistor. Original
The reduced graphene oxide-based biosensor according to Claim 1, wherein said magnetic nanoparticle is selected from a group consisting of a Fe₃ 0 4 magnetic nanoparticle, a Ni magnetic nanoparticle, and a combination thereof. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said composite has a size ranging from 30 nm to 50 nm. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said reduced graphene oxide having said linking moiety is made by subjecting a cyclic dianhydride compound and a graphite to a Friedel-Crafts reaction in the presence of a Lewis acid. Original
A method for detecting an analyte using the according to Claim 1, comprising the steps of: region through a gate electrode of the reduced reduced graphene oxide-based biosensor into contact current of the reduced graphene oxide-based oxide-based biosensor is brought with contact with the analyte. Withdrawn reduced graphene oxide-based biosensor applying a predetermined potential to a channel graphene oxide-based biosensor; bringing the with the analyte; and measuring a change in biosensor before and after the reduced graphene the analyte for determining a concentration of
. Canceled
. Canceled
The reduced graphene oxide-based biosensor according to Claim [[4]] 1, wherein said antibody is an anti-APOA₂ antibody. Currently amended
Embodiments described in the patent, grouped by the materials and process steps they use.
3 materials1 process step
Preparation of reduced graphene oxide (GLA11, GLA13, GLA₁₆) having a linking moiety of -(C=O)-X-COOH using AlCl₃ (Lewis acid) and maleic anhydride (MA) via Friedel-Crafts reaction on graphite.
Layer stacks claimed or described, ordered top of device to substrate.
reduced graphene oxide-based biosensor with nanostructure field-effect transistor
polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET)
Materials described outside the worked examples.
antibody specific to analyte
composite of reduced graphene oxide and magnetic nanoparticle
anti-APOA₂ antibody
Measurements and analyses referenced in the patent, with their drawing references.
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
composite size range | 30–50 nm | composite of reduced graphene oxide and magnetic nanoparticle |
magnetic nanoparticle average particle diameter | 4–50 nm |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,598,658Patent drawings and their descriptions. Click a drawing to enlarge it.
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Figure 2 is a photo illustrating a comparison of the solubilities of graphite, a modified graphene product (GLA 1 3) of Preparation Example 1, and a modified graphene product (GSA) of Comparative Preparation Example 1 in water;
Figure 3 is a plot illustrating absorption spectra of graphite, modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and a modified 20 graphene product (GSA) of Comparative Preparation Example 1
Figure 4 is a plot illustrating an X-ray photoelectron spectrum of graphite;
Figure 5 is a plot illustrating an X-ray photoelectron spectrum of the modified graphene product (GLA 1 3) of Preparation Example 2
Figure 6 is a plot illustrating Fourier transform infrared (FT-IR) spectra of 25 graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 7 is a bar chart illustrating a comparison of the hydroxyl group densities of graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation 4 PATENTS 218091-0014 PE-51941-AM Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 8 is a plot illustrating thermogravimetric analysis spectra of graphite, the modified graphene product (GLA 1 3) of Preparation Example 2, and the modified 0 graphene product (GSA) of Comparative Preparation Example 1
Figure 9 is a plot illustrating X-ray diffraction (XRD) spectra of Ni, graphite, and the modified graphene products (GLA 1 1, GLA 13, and GLA 16) of Preparation Examples 1 -3;
Figure 10 is a transmission electron microscopic (TEM) image illustrating a 10 structure of the modified graphene product (GLA 1 1) of Preparation Example 1
Figure 11 is a TEM image illustrating a structure of the modified graphene product (GLA₁₃) of Preparation Example 2
Figure 12 is a TEM image illustrating a structure of the modified graphene product (GLA₁₆) of Preparation Example 3 0
Figure 13 is a TEM image illustrating a structure of the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 14 is a plot illustrating absorption spectra of iron oxide (Fe 3 0 4), the modified graphene product (GLA 1 3) of Preparation Example 2, and the magnetizied modified graphene product (MGLA) of Preparation Example 4 20
Figure 15 is a photo illustrating magnetic analysis results for the modified graphene product (GLA₁₃) of Preparation Example 2 and the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 16 is a plot illustrating XRD spectra of the modified graphene product (GLA 1 3) of Preparation Example 2 and the magnetized modified graphene product 25 (MGLA) of Preparation
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A reduced graphene oxide-based biosensor comprising a na no -structure field-effect transistor, said transistor including a channel region that contains a composite formed [from] of (i) a reduced graphene oxide having a linking moiety of the formula -(C =O)-X- C OOH, X being a C 2 -C₃ alkenylene group or a C₁ -C 3 alkylene group, bonded to an antibody specific to an analyte, a-C 4 -C3; and (ii) a magnetic nanoparticle adsorbed onto said reduced graphene oxide. Currently amended
The reduced graphene oxide-based biosensor according to Claim 1, wherein said channel region further contains an immobilization layer for immobilizing said composite. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein X is a C 2 -C 3 alkenylene group. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said nanostructure field-effect transistor is a polycrystalline silicon nanowire field-effect transistor. Original
The reduced graphene oxide-based biosensor according to Claim 1, wherein said magnetic nanoparticle is selected from a group consisting of a Fe₃ 0 4 magnetic nanoparticle, a Ni magnetic nanoparticle, and a combination thereof. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said composite has a size ranging from 30 nm to 50 nm. Previously presented
The reduced graphene oxide-based biosensor according to Claim 1, wherein said reduced graphene oxide having said linking moiety is made by subjecting a cyclic dianhydride compound and a graphite to a Friedel-Crafts reaction in the presence of a Lewis acid. Original
A method for detecting an analyte using the according to Claim 1, comprising the steps of: region through a gate electrode of the reduced reduced graphene oxide-based biosensor into contact current of the reduced graphene oxide-based oxide-based biosensor is brought with contact with the analyte. Withdrawn reduced graphene oxide-based biosensor applying a predetermined potential to a channel graphene oxide-based biosensor; bringing the with the analyte; and measuring a change in biosensor before and after the reduced graphene the analyte for determining a concentration of
. Canceled
. Canceled
The reduced graphene oxide-based biosensor according to Claim [[4]] 1, wherein said antibody is an anti-APOA₂ antibody. Currently amended
Embodiments described in the patent, grouped by the materials and process steps they use.
3 materials1 process step
Preparation of reduced graphene oxide (GLA11, GLA13, GLA₁₆) having a linking moiety of -(C=O)-X-COOH using AlCl₃ (Lewis acid) and maleic anhydride (MA) via Friedel-Crafts reaction on graphite.
Layer stacks claimed or described, ordered top of device to substrate.
reduced graphene oxide-based biosensor with nanostructure field-effect transistor
polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET)
Materials described outside the worked examples.
antibody specific to analyte
composite of reduced graphene oxide and magnetic nanoparticle
anti-APOA₂ antibody
Measurements and analyses referenced in the patent, with their drawing references.
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
composite size range | 30–50 nm | composite of reduced graphene oxide and magnetic nanoparticle |
magnetic nanoparticle average particle diameter | 4–50 nm |
Related documents with shared materials, methods, properties, or citations.
Fe₃O₄ magnetic nanoparticle
Fe₃O₄
Ni magnetic nanoparticle
Ni
aminosilane compound
dialdehyde compound
aluminum chloride (AlCl₃)
AlCl₃
3-aminopropyltriethoxysilane (APTES)
glutaraldehyde (GA)
polycrystalline silicon nanowire
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Figure 3 is a plot illustrating absorption spectra of graphite, modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and a modified 20 graphene product (GSA) of Comparative Preparation Example 1
Figure 4 is a plot illustrating an X-ray photoelectron spectrum of graphite;
Figure 5 is a plot illustrating an X-ray photoelectron spectrum of the modified graphene product (GLA 1 3) of Preparation Example 2
Figure 6 is a plot illustrating Fourier transform infrared (FT-IR) spectra of 25 graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 9 is a plot illustrating X-ray diffraction (XRD) spectra of Ni, graphite, and the modified graphene products (GLA 1 1, GLA 13, and GLA 16) of Preparation Examples 1 -3;
Figure 10 is a transmission electron microscopic (TEM) image illustrating a 10 structure of the modified graphene product (GLA 1 1) of Preparation Example 1
Figure 11 is a TEM image illustrating a structure of the modified graphene product (GLA₁₃) of Preparation Example 2
Figure 12 is a TEM image illustrating a structure of the modified graphene product (GLA₁₆) of Preparation Example 3 0
Figure 13 is a TEM image illustrating a structure of the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 14 is a plot illustrating absorption spectra of iron oxide (Fe 3 0 4), the modified graphene product (GLA 1 3) of Preparation Example 2, and the magnetizied modified graphene product (MGLA) of Preparation Example 4 20
Figure 16 is a plot illustrating XRD spectra of the modified graphene product (GLA 1 3) of Preparation Example 2 and the magnetized modified graphene product 25 (MGLA) of Preparation
Fe₃O₄ |
Thickness | 4–10 nm | — |
Thickness | 200–900 nm | — |
Voltage | 0–3 V | — |
Thickness | ≥ 300 nm | — |
Fe₃O₄ magnetic nanoparticle
Fe₃O₄
Ni magnetic nanoparticle
Ni
aminosilane compound
dialdehyde compound
aluminum chloride (AlCl₃)
AlCl₃
3-aminopropyltriethoxysilane (APTES)
glutaraldehyde (GA)
polycrystalline silicon nanowire
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Figure 3 is a plot illustrating absorption spectra of graphite, modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and a modified 20 graphene product (GSA) of Comparative Preparation Example 1
Figure 4 is a plot illustrating an X-ray photoelectron spectrum of graphite;
Figure 5 is a plot illustrating an X-ray photoelectron spectrum of the modified graphene product (GLA 1 3) of Preparation Example 2
Figure 6 is a plot illustrating Fourier transform infrared (FT-IR) spectra of 25 graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 9 is a plot illustrating X-ray diffraction (XRD) spectra of Ni, graphite, and the modified graphene products (GLA 1 1, GLA 13, and GLA 16) of Preparation Examples 1 -3;
Figure 10 is a transmission electron microscopic (TEM) image illustrating a 10 structure of the modified graphene product (GLA 1 1) of Preparation Example 1
Figure 11 is a TEM image illustrating a structure of the modified graphene product (GLA₁₃) of Preparation Example 2
Figure 12 is a TEM image illustrating a structure of the modified graphene product (GLA₁₆) of Preparation Example 3 0
Figure 13 is a TEM image illustrating a structure of the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 14 is a plot illustrating absorption spectra of iron oxide (Fe 3 0 4), the modified graphene product (GLA 1 3) of Preparation Example 2, and the magnetizied modified graphene product (MGLA) of Preparation Example 4 20
Figure 16 is a plot illustrating XRD spectra of the modified graphene product (GLA 1 3) of Preparation Example 2 and the magnetized modified graphene product 25 (MGLA) of Preparation
Fe₃O₄ |
Thickness | 4–10 nm | — |
Thickness | 200–900 nm | — |
Voltage | 0–3 V | — |
Thickness | ≥ 300 nm | — |
Fe₃O₄ magnetic nanoparticle
Fe₃O₄
Ni magnetic nanoparticle
Ni
aminosilane compound
dialdehyde compound
aluminum chloride (AlCl₃)
AlCl₃
3-aminopropyltriethoxysilane (APTES)
glutaraldehyde (GA)
polycrystalline silicon nanowire
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Figure 3 is a plot illustrating absorption spectra of graphite, modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and a modified 20 graphene product (GSA) of Comparative Preparation Example 1
Figure 4 is a plot illustrating an X-ray photoelectron spectrum of graphite;
Figure 5 is a plot illustrating an X-ray photoelectron spectrum of the modified graphene product (GLA 1 3) of Preparation Example 2
Figure 6 is a plot illustrating Fourier transform infrared (FT-IR) spectra of 25 graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 9 is a plot illustrating X-ray diffraction (XRD) spectra of Ni, graphite, and the modified graphene products (GLA 1 1, GLA 13, and GLA 16) of Preparation Examples 1 -3;
Figure 10 is a transmission electron microscopic (TEM) image illustrating a 10 structure of the modified graphene product (GLA 1 1) of Preparation Example 1
Figure 11 is a TEM image illustrating a structure of the modified graphene product (GLA₁₃) of Preparation Example 2
Figure 12 is a TEM image illustrating a structure of the modified graphene product (GLA₁₆) of Preparation Example 3 0
Figure 13 is a TEM image illustrating a structure of the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 14 is a plot illustrating absorption spectra of iron oxide (Fe 3 0 4), the modified graphene product (GLA 1 3) of Preparation Example 2, and the magnetizied modified graphene product (MGLA) of Preparation Example 4 20
Figure 16 is a plot illustrating XRD spectra of the modified graphene product (GLA 1 3) of Preparation Example 2 and the magnetized modified graphene product 25 (MGLA) of Preparation
Fe₃O₄ |
Thickness | 4–10 nm | — |
Thickness | 200–900 nm | — |
Voltage | 0–3 V | — |
Thickness | ≥ 300 nm | — |
Fe₃O₄ magnetic nanoparticle
Fe₃O₄
Ni magnetic nanoparticle
Ni
aminosilane compound
dialdehyde compound
aluminum chloride (AlCl₃)
AlCl₃
3-aminopropyltriethoxysilane (APTES)
glutaraldehyde (GA)
polycrystalline silicon nanowire
Figure 1 is a scanning electron microscope (SEM) image illustrating a microscopic structure of an N-type polycrystalline silicon nanowire field effect transistor used in a biosensor of Example 1 0
Figure 3 is a plot illustrating absorption spectra of graphite, modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and a modified 20 graphene product (GSA) of Comparative Preparation Example 1
Figure 4 is a plot illustrating an X-ray photoelectron spectrum of graphite;
Figure 5 is a plot illustrating an X-ray photoelectron spectrum of the modified graphene product (GLA 1 3) of Preparation Example 2
Figure 6 is a plot illustrating Fourier transform infrared (FT-IR) spectra of 25 graphite, the modified graphene products (GLA 1 1, GLA 1 3, and GLA 1 6) of Preparation Examples 1 -3, and the modified graphene product (GSA) of Comparative Preparation Example 1
Figure 9 is a plot illustrating X-ray diffraction (XRD) spectra of Ni, graphite, and the modified graphene products (GLA 1 1, GLA 13, and GLA 16) of Preparation Examples 1 -3;
Figure 10 is a transmission electron microscopic (TEM) image illustrating a 10 structure of the modified graphene product (GLA 1 1) of Preparation Example 1
Figure 11 is a TEM image illustrating a structure of the modified graphene product (GLA₁₃) of Preparation Example 2
Figure 12 is a TEM image illustrating a structure of the modified graphene product (GLA₁₆) of Preparation Example 3 0
Figure 13 is a TEM image illustrating a structure of the magnetized modified graphene product (MGLA) of Preparation Example 4
Figure 14 is a plot illustrating absorption spectra of iron oxide (Fe 3 0 4), the modified graphene product (GLA 1 3) of Preparation Example 2, and the magnetizied modified graphene product (MGLA) of Preparation Example 4 20
Figure 16 is a plot illustrating XRD spectra of the modified graphene product (GLA 1 3) of Preparation Example 2 and the magnetized modified graphene product 25 (MGLA) of Preparation
Fe₃O₄ |
Thickness | 4–10 nm | — |
Thickness | 200–900 nm | — |
Voltage | 0–3 V | — |
Thickness | ≥ 300 nm | — |