The preparation of the composite which was immobilized Paracoccus denitr ifz cans on modified graphene oxide, comprises: 1) synthesis of graphene oxide: add graphite into concentrated H 2 S O 4 in accordance with the ratio of every 1 g graphite 20-25 mL concentrated H 2 S O 4, while cooling with an ice bath to 0 0 C and stirred continually, then, in accordance with mass ratio graphite: KMnO 4 = 1:5-8, add KMnO 4 to the suspension in batch, and keep the reaction system at 35-40 0 C and stirr for 15-20 h, after that, pour the reaction mixture into ice water containing H 2 0 2, and centrifuge, wash and dry to obtain graphene oxide; 2) synthesis of modified graphene oxide: in accordance with graphene oxide: methacryloyl chloride = 1: 0.5-1.5 in mass ratio and triethylamine: methacryloyl chloride = 1: 1 in stoichiometric molar ratio, disperse said graphene oxide obtained in step 1) in dimethylacetamide with triethylamine under inert gas conditions at 0 0 C and stir continually, then add the mixture of methacryloyl chloride and dimethylacetamide to the reaction system and keep at room temperature and stir for 20 -24 h, subsequently, centrifuge, wash and dry the product to obtain acylated graphene oxide; in accordance with acylated graphene oxide: methacrylic acid = 1: 0.5-2.5 in mass ratio and methacrylic acid: butyl methacrylate: azobisisobutyronitrile = 50: 50-100: 1-2 in stoichiometric molar ratio, disperse the acylated graphene oxide in DMF under inert gas conditions, then, add methacrylic acid and butyl methacrylate as the monomers mixture, and the initiator into the reaction system, proceed the free radical solution polymerization at 60-70 0 C for 8-10 h, precipitate the mixture in ether, and then filter and dry to obtain PGO; 3) acclimatization and immobilization of Paracoccus denitr i ficans: transfer the cells of Paracoccus denitr ifi cans which were harvested in liquid medium with 1000 mg/L of glucose solution, and the acclimatization process was achieved by following method: 10 Docket No. 10001.0021 increase the D M F concentration with 100 mg/L while decreasing the glucose concentration with 100 mg/L per 3-6 days, while the glucose concentration reaches 0 mg/L, change the liquid medium, and constantly increase the DMF concentration up to 2000 mg/L and completed the acclimatization process; in accordance with PGO: N-hydroxy succinimide: N,N'- Dicyclohexylcarbodiimide = 1: 1-2: 1-3 in mass ratio, and N,N'-Dicyclohexylcarbodiimide: 4-dimethylaminopyridine = 1: 1 in stoichiometric molar ratio, disperse PGO obtained in step 2) and N-hydroxy succinimide in DMF, then add N,N'-Dicyclohexylcarbodiimide and 4-dimethylaminopyridine, stir for 24 h at room temperature to obtain amidated PGO; in accordance with amidated PGO: acclimatized bacteria = 1: 1-3 in mass ratio, add the amidated PGO and acclimatized bacteria into the Phosphate Buffered Saline solution and incubate at 30 0 C for 20-24 h to get composite which immobilized Paracoccus denitr ifi cans on modified graphene oxide.

The preparation of the composite which was immobilized Paracoccus denitr ifz cans on modified graphene oxide, comprises: 1) synthesis of graphene oxide: add graphite into concentrated H 2 S O 4 in accordance with the ratio of every 1 g graphite 20-25 mL concentrated H 2 S O 4, while cooling with an ice bath to 0 0 C and stirred continually, then, in accordance with mass ratio graphite: KMnO 4 = 1:5-8, add KMnO 4 to the suspension in batch, and keep the reaction system at 35-40 0 C and stirr for 15-20 h, after that, pour the reaction mixture into ice water containing H 2 0 2, and centrifuge, wash and dry to obtain graphene oxide; 2) synthesis of modified graphene oxide: in accordance with graphene oxide: methacryloyl chloride = 1: 0.5-1.5 in mass ratio and triethylamine: methacryloyl chloride = 1: 1 in stoichiometric molar ratio, disperse said graphene oxide obtained in step 1) in dimethylacetamide with triethylamine under inert gas conditions at 0 0 C and stir continually, then add the mixture of methacryloyl chloride and dimethylacetamide to the reaction system and keep at room temperature and stir for 20 -24 h, subsequently, centrifuge, wash and dry the product to obtain acylated graphene oxide; in accordance with acylated graphene oxide: methacrylic acid = 1: 0.5-2.5 in mass ratio and methacrylic acid: butyl methacrylate: azobisisobutyronitrile = 50: 50-100: 1-2 in stoichiometric molar ratio, disperse the acylated graphene oxide in DMF under inert gas conditions, then, add methacrylic acid and butyl methacrylate as the monomers mixture, and the initiator into the reaction system, proceed the free radical solution polymerization at 60-70 0 C for 8-10 h, precipitate the mixture in ether, and then filter and dry to obtain PGO; 3) acclimatization and immobilization of Paracoccus denitr i ficans: transfer the cells of Paracoccus denitr ifi cans which were harvested in liquid medium with 1000 mg/L of glucose solution, and the acclimatization process was achieved by following method: 10 Docket No. 10001.0021 increase the D M F concentration with 100 mg/L while decreasing the glucose concentration with 100 mg/L per 3-6 days, while the glucose concentration reaches 0 mg/L, change the liquid medium, and constantly increase the DMF concentration up to 2000 mg/L and completed the acclimatization process; in accordance with PGO: N-hydroxy succinimide: N,N'- Dicyclohexylcarbodiimide = 1: 1-2: 1-3 in mass ratio, and N,N'-Dicyclohexylcarbodiimide: 4-dimethylaminopyridine = 1: 1 in stoichiometric molar ratio, disperse PGO obtained in step 2) and N-hydroxy succinimide in DMF, then add N,N'-Dicyclohexylcarbodiimide and 4-dimethylaminopyridine, stir for 24 h at room temperature to obtain amidated PGO; in accordance with amidated PGO: acclimatized bacteria = 1: 1-3 in mass ratio, add the amidated PGO and acclimatized bacteria into the Phosphate Buffered Saline solution and incubate at 30 0 C for 20-24 h to get composite which immobilized Paracoccus denitr ifi cans on modified graphene oxide.

The preparation of the composite which was immobilized Paracoccus denitr ifz cans on modified graphene oxide, comprises: 1) synthesis of graphene oxide: add graphite into concentrated H 2 S O 4 in accordance with the ratio of every 1 g graphite 20-25 mL concentrated H 2 S O 4, while cooling with an ice bath to 0 0 C and stirred continually, then, in accordance with mass ratio graphite: KMnO 4 = 1:5-8, add KMnO 4 to the suspension in batch, and keep the reaction system at 35-40 0 C and stirr for 15-20 h, after that, pour the reaction mixture into ice water containing H 2 0 2, and centrifuge, wash and dry to obtain graphene oxide; 2) synthesis of modified graphene oxide: in accordance with graphene oxide: methacryloyl chloride = 1: 0.5-1.5 in mass ratio and triethylamine: methacryloyl chloride = 1: 1 in stoichiometric molar ratio, disperse said graphene oxide obtained in step 1) in dimethylacetamide with triethylamine under inert gas conditions at 0 0 C and stir continually, then add the mixture of methacryloyl chloride and dimethylacetamide to the reaction system and keep at room temperature and stir for 20 -24 h, subsequently, centrifuge, wash and dry the product to obtain acylated graphene oxide; in accordance with acylated graphene oxide: methacrylic acid = 1: 0.5-2.5 in mass ratio and methacrylic acid: butyl methacrylate: azobisisobutyronitrile = 50: 50-100: 1-2 in stoichiometric molar ratio, disperse the acylated graphene oxide in DMF under inert gas conditions, then, add methacrylic acid and butyl methacrylate as the monomers mixture, and the initiator into the reaction system, proceed the free radical solution polymerization at 60-70 0 C for 8-10 h, precipitate the mixture in ether, and then filter and dry to obtain PGO; 3) acclimatization and immobilization of Paracoccus denitr i ficans: transfer the cells of Paracoccus denitr ifi cans which were harvested in liquid medium with 1000 mg/L of glucose solution, and the acclimatization process was achieved by following method: 10 Docket No. 10001.0021 increase the D M F concentration with 100 mg/L while decreasing the glucose concentration with 100 mg/L per 3-6 days, while the glucose concentration reaches 0 mg/L, change the liquid medium, and constantly increase the DMF concentration up to 2000 mg/L and completed the acclimatization process; in accordance with PGO: N-hydroxy succinimide: N,N'- Dicyclohexylcarbodiimide = 1: 1-2: 1-3 in mass ratio, and N,N'-Dicyclohexylcarbodiimide: 4-dimethylaminopyridine = 1: 1 in stoichiometric molar ratio, disperse PGO obtained in step 2) and N-hydroxy succinimide in DMF, then add N,N'-Dicyclohexylcarbodiimide and 4-dimethylaminopyridine, stir for 24 h at room temperature to obtain amidated PGO; in accordance with amidated PGO: acclimatized bacteria = 1: 1-3 in mass ratio, add the amidated PGO and acclimatized bacteria into the Phosphate Buffered Saline solution and incubate at 30 0 C for 20-24 h to get composite which immobilized Paracoccus denitr ifi cans on modified graphene oxide.

The preparation of the composite which was immobilized Paracoccus denitr ifz cans on modified graphene oxide, comprises: 1) synthesis of graphene oxide: add graphite into concentrated H 2 S O 4 in accordance with the ratio of every 1 g graphite 20-25 mL concentrated H 2 S O 4, while cooling with an ice bath to 0 0 C and stirred continually, then, in accordance with mass ratio graphite: KMnO 4 = 1:5-8, add KMnO 4 to the suspension in batch, and keep the reaction system at 35-40 0 C and stirr for 15-20 h, after that, pour the reaction mixture into ice water containing H 2 0 2, and centrifuge, wash and dry to obtain graphene oxide; 2) synthesis of modified graphene oxide: in accordance with graphene oxide: methacryloyl chloride = 1: 0.5-1.5 in mass ratio and triethylamine: methacryloyl chloride = 1: 1 in stoichiometric molar ratio, disperse said graphene oxide obtained in step 1) in dimethylacetamide with triethylamine under inert gas conditions at 0 0 C and stir continually, then add the mixture of methacryloyl chloride and dimethylacetamide to the reaction system and keep at room temperature and stir for 20 -24 h, subsequently, centrifuge, wash and dry the product to obtain acylated graphene oxide; in accordance with acylated graphene oxide: methacrylic acid = 1: 0.5-2.5 in mass ratio and methacrylic acid: butyl methacrylate: azobisisobutyronitrile = 50: 50-100: 1-2 in stoichiometric molar ratio, disperse the acylated graphene oxide in DMF under inert gas conditions, then, add methacrylic acid and butyl methacrylate as the monomers mixture, and the initiator into the reaction system, proceed the free radical solution polymerization at 60-70 0 C for 8-10 h, precipitate the mixture in ether, and then filter and dry to obtain PGO; 3) acclimatization and immobilization of Paracoccus denitr i ficans: transfer the cells of Paracoccus denitr ifi cans which were harvested in liquid medium with 1000 mg/L of glucose solution, and the acclimatization process was achieved by following method: 10 Docket No. 10001.0021 increase the D M F concentration with 100 mg/L while decreasing the glucose concentration with 100 mg/L per 3-6 days, while the glucose concentration reaches 0 mg/L, change the liquid medium, and constantly increase the DMF concentration up to 2000 mg/L and completed the acclimatization process; in accordance with PGO: N-hydroxy succinimide: N,N'- Dicyclohexylcarbodiimide = 1: 1-2: 1-3 in mass ratio, and N,N'-Dicyclohexylcarbodiimide: 4-dimethylaminopyridine = 1: 1 in stoichiometric molar ratio, disperse PGO obtained in step 2) and N-hydroxy succinimide in DMF, then add N,N'-Dicyclohexylcarbodiimide and 4-dimethylaminopyridine, stir for 24 h at room temperature to obtain amidated PGO; in accordance with amidated PGO: acclimatized bacteria = 1: 1-3 in mass ratio, add the amidated PGO and acclimatized bacteria into the Phosphate Buffered Saline solution and incubate at 30 0 C for 20-24 h to get composite which immobilized Paracoccus denitr ifi cans on modified graphene oxide.