Patent
Atlas literature
Patent
US 10,809,222Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 3 SVG 14786674.10-23-2015.IGR₇SPUAPXXIFW4.ABST.1.48.233.3045.278.3074.svg 0.097 0.15 Chemistry Black and white SVG …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of detecting the presence of an analyte and/or determining analyte concentration in a sample, comprising: applying a voltage to a graphene field effect transistor sensor having a conduction channel and comprising a detection molecule being bound by an amide bond to the graphene; measuring a current of the sensor; exposing the conduction channel to the sample; measuring the current of the sensor; determining the presence and/or concentration of the analyte based upon a difference in the current; and determining the conformational changes of the receptor upon analyte binding. Withdrawn
The method of claim 1, wherein at least one detection molecule comprises a G-protein-coupled-receptor molecule, an enzyme, a peptide, an endorphin, a receptor, a ligand, or any combination thereof. Withdrawn
6-21 Canceled
Canceled
A sensing device, comprising a graphene portion placing first and second electrodes into electronic communication with one another, the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D p eak that is less than a G p eak when assessed using Raman spectroscopy, the detection molecule being in electronic communication with the graphene and being bound to the graphene portion by non-covalent pi-pi stacking interaction between the graphene and a 1 -pyrene butanoic acid succinimidyl ester molecule and an amide bond between the 1- pyrene butanoic acid succinimidyl ester molecule and the detection molecule, and, the detection molecule being an integral membrane receptor molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target. Currently amended
The sensing device of claim 22, the sensing device being configured such that the device, during operation, is capable of detecting a change in signal related to the complementary binding between the detection molecule at the at least one target. Original
The sensing device of claim 22, further comprising a voltage source configured to apply a voltage across the first and second electrodes. Original
A sensing device of claim 22, wherein the detection molecule comprises a protein having an amino acid sequence that is at least about 95 % identical to any one of SEQ ID 1 through SEQ ID
A multiplexed device, comprising: a substrate having a plurality of sensing devices according to claim 22 disposed thereon, at least two of the sensing device comprising different detection molecules. Original
A method of functionalizing a sensing device according to claim 22, comprising: exposing the graphene portion to a solution of carboxylated diazonium salt to create carboxylate groups on the graphene portion; activating the carboxylate groups with a carbodiimide; stabilizing the carboxylate groups with N H S molecules; and displacing the N H S molecules with detection molecules having an accessible amine group so as to create an amide bond between the graphene portion and a receptor molecule. Withdrawn
A method of functionalizing a sensing device according to claim 22, comprising: exposing the graphene portion to Ni-nitrilotriacetic acid molecules having Ni 2+ cations so to create bonds between the nitrilotriacetic acid portion and the graphene; and exposing the Ni 2+ cations to detection molecules having an accessible histidine tag so as to create bonds between the Ni 2+ cations and the detection molecule. Withdrawn
The sensing device of claim 22, wherein the detection molecule comprises protein UniProtK B: P07550 or an associated variant with the capability to bind opioid ligands. Previously presented
Canceled
-29. Canceled
Canceled
-37. Canceled
Canceled
A sensing device, comprising: a graphene portion placing first and second electrodes into electronic communication with one another, the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D p eak that is less than a G p eak when assessed using Raman spectroscopy, the detection molecule being in electronic communication with the graphene, the detection molecule being a recombinant integral membrane protein molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target. Currently amended
Layer stacks claimed or described, ordered top of device to substrate.
graphene field effect transistor (GFET) sensor with amide-bonded detection molecule
GFET sensing device with pi-pi stacked 1-PBASE linker and mu receptor detection molecule
Materials described outside the worked examples.
graphene
1-pyrene butanoic acid succinimidyl ester
amide bond linkage
Additional fabrication and treatment steps described in the patent.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
D peak less than G peak (Raman spectroscopy) — graphene quality indicator | — | graphene |
Thickness | 20–100 µm |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,809,222Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 3 SVG 14786674.10-23-2015.IGR₇SPUAPXXIFW4.ABST.1.48.233.3045.278.3074.svg 0.097 0.15 Chemistry Black and white SVG …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of detecting the presence of an analyte and/or determining analyte concentration in a sample, comprising: applying a voltage to a graphene field effect transistor sensor having a conduction channel and comprising a detection molecule being bound by an amide bond to the graphene; measuring a current of the sensor; exposing the conduction channel to the sample; measuring the current of the sensor; determining the presence and/or concentration of the analyte based upon a difference in the current; and determining the conformational changes of the receptor upon analyte binding. Withdrawn
The method of claim 1, wherein at least one detection molecule comprises a G-protein-coupled-receptor molecule, an enzyme, a peptide, an endorphin, a receptor, a ligand, or any combination thereof. Withdrawn
6-21 Canceled
Canceled
A sensing device, comprising a graphene portion placing first and second electrodes into electronic communication with one another, the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D p eak that is less than a G p eak when assessed using Raman spectroscopy, the detection molecule being in electronic communication with the graphene and being bound to the graphene portion by non-covalent pi-pi stacking interaction between the graphene and a 1 -pyrene butanoic acid succinimidyl ester molecule and an amide bond between the 1- pyrene butanoic acid succinimidyl ester molecule and the detection molecule, and, the detection molecule being an integral membrane receptor molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target. Currently amended
The sensing device of claim 22, the sensing device being configured such that the device, during operation, is capable of detecting a change in signal related to the complementary binding between the detection molecule at the at least one target. Original
The sensing device of claim 22, further comprising a voltage source configured to apply a voltage across the first and second electrodes. Original
A sensing device of claim 22, wherein the detection molecule comprises a protein having an amino acid sequence that is at least about 95 % identical to any one of SEQ ID 1 through SEQ ID
A multiplexed device, comprising: a substrate having a plurality of sensing devices according to claim 22 disposed thereon, at least two of the sensing device comprising different detection molecules. Original
A method of functionalizing a sensing device according to claim 22, comprising: exposing the graphene portion to a solution of carboxylated diazonium salt to create carboxylate groups on the graphene portion; activating the carboxylate groups with a carbodiimide; stabilizing the carboxylate groups with N H S molecules; and displacing the N H S molecules with detection molecules having an accessible amine group so as to create an amide bond between the graphene portion and a receptor molecule. Withdrawn
A method of functionalizing a sensing device according to claim 22, comprising: exposing the graphene portion to Ni-nitrilotriacetic acid molecules having Ni 2+ cations so to create bonds between the nitrilotriacetic acid portion and the graphene; and exposing the Ni 2+ cations to detection molecules having an accessible histidine tag so as to create bonds between the Ni 2+ cations and the detection molecule. Withdrawn
The sensing device of claim 22, wherein the detection molecule comprises protein UniProtK B: P07550 or an associated variant with the capability to bind opioid ligands. Previously presented
Canceled
-29. Canceled
Canceled
-37. Canceled
Canceled
A sensing device, comprising: a graphene portion placing first and second electrodes into electronic communication with one another, the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D p eak that is less than a G p eak when assessed using Raman spectroscopy, the detection molecule being in electronic communication with the graphene, the detection molecule being a recombinant integral membrane protein molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target. Currently amended
Layer stacks claimed or described, ordered top of device to substrate.
graphene field effect transistor (GFET) sensor with amide-bonded detection molecule
GFET sensing device with pi-pi stacked 1-PBASE linker and mu receptor detection molecule
Materials described outside the worked examples.
graphene
1-pyrene butanoic acid succinimidyl ester
amide bond linkage
Additional fabrication and treatment steps described in the patent.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
D peak less than G peak (Raman spectroscopy) — graphene quality indicator | — | graphene |
Thickness | 20–100 µm |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,809,222Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 3 SVG 14786674.10-23-2015.IGR₇SPUAPXXIFW4.ABST.1.48.233.3045.278.3074.svg 0.097 0.15 Chemistry Black and white SVG …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of detecting the presence of an analyte and/or determining analyte concentration in a sample, comprising: applying a voltage to a graphene field effect transistor sensor having a conduction channel and comprising a detection molecule being bound by an amide bond to the graphene; measuring a current of the sensor; exposing the conduction channel to the sample; measuring the current of the sensor; determining the presence and/or concentration of the analyte based upon a difference in the current; and determining the conformational changes of the receptor upon analyte binding. Withdrawn
The method of claim 1, wherein at least one detection molecule comprises a G-protein-coupled-receptor molecule, an enzyme, a peptide, an endorphin, a receptor, a ligand, or any combination thereof. Withdrawn
6-21 Canceled
Canceled
A sensing device, comprising a graphene portion placing first and second electrodes into electronic communication with one another, the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D p eak that is less than a G p eak when assessed using Raman spectroscopy, the detection molecule being in electronic communication with the graphene and being bound to the graphene portion by non-covalent pi-pi stacking interaction between the graphene and a 1 -pyrene butanoic acid succinimidyl ester molecule and an amide bond between the 1- pyrene butanoic acid succinimidyl ester molecule and the detection molecule, and, the detection molecule being an integral membrane receptor molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target. Currently amended
The sensing device of claim 22, the sensing device being configured such that the device, during operation, is capable of detecting a change in signal related to the complementary binding between the detection molecule at the at least one target. Original
The sensing device of claim 22, further comprising a voltage source configured to apply a voltage across the first and second electrodes. Original
A sensing device of claim 22, wherein the detection molecule comprises a protein having an amino acid sequence that is at least about 95 % identical to any one of SEQ ID 1 through SEQ ID
A multiplexed device, comprising: a substrate having a plurality of sensing devices according to claim 22 disposed thereon, at least two of the sensing device comprising different detection molecules. Original
A method of functionalizing a sensing device according to claim 22, comprising: exposing the graphene portion to a solution of carboxylated diazonium salt to create carboxylate groups on the graphene portion; activating the carboxylate groups with a carbodiimide; stabilizing the carboxylate groups with N H S molecules; and displacing the N H S molecules with detection molecules having an accessible amine group so as to create an amide bond between the graphene portion and a receptor molecule. Withdrawn
A method of functionalizing a sensing device according to claim 22, comprising: exposing the graphene portion to Ni-nitrilotriacetic acid molecules having Ni 2+ cations so to create bonds between the nitrilotriacetic acid portion and the graphene; and exposing the Ni 2+ cations to detection molecules having an accessible histidine tag so as to create bonds between the Ni 2+ cations and the detection molecule. Withdrawn
The sensing device of claim 22, wherein the detection molecule comprises protein UniProtK B: P07550 or an associated variant with the capability to bind opioid ligands. Previously presented
Canceled
-29. Canceled
Canceled
-37. Canceled
Canceled
A sensing device, comprising: a graphene portion placing first and second electrodes into electronic communication with one another, the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D p eak that is less than a G p eak when assessed using Raman spectroscopy, the detection molecule being in electronic communication with the graphene, the detection molecule being a recombinant integral membrane protein molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target. Currently amended
Layer stacks claimed or described, ordered top of device to substrate.
graphene field effect transistor (GFET) sensor with amide-bonded detection molecule
GFET sensing device with pi-pi stacked 1-PBASE linker and mu receptor detection molecule
Materials described outside the worked examples.
graphene
1-pyrene butanoic acid succinimidyl ester
amide bond linkage
Additional fabrication and treatment steps described in the patent.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
D peak less than G peak (Raman spectroscopy) — graphene quality indicator | — | graphene |
Thickness | 20–100 µm |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 10,809,222Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 3 SVG 14786674.10-23-2015.IGR₇SPUAPXXIFW4.ABST.1.48.233.3045.278.3074.svg 0.097 0.15 Chemistry Black and white SVG …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method of detecting the presence of an analyte and/or determining analyte concentration in a sample, comprising: applying a voltage to a graphene field effect transistor sensor having a conduction channel and comprising a detection molecule being bound by an amide bond to the graphene; measuring a current of the sensor; exposing the conduction channel to the sample; measuring the current of the sensor; determining the presence and/or concentration of the analyte based upon a difference in the current; and determining the conformational changes of the receptor upon analyte binding. Withdrawn
The method of claim 1, wherein at least one detection molecule comprises a G-protein-coupled-receptor molecule, an enzyme, a peptide, an endorphin, a receptor, a ligand, or any combination thereof. Withdrawn
6-21 Canceled
Canceled
A sensing device, comprising a graphene portion placing first and second electrodes into electronic communication with one another, the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D p eak that is less than a G p eak when assessed using Raman spectroscopy, the detection molecule being in electronic communication with the graphene and being bound to the graphene portion by non-covalent pi-pi stacking interaction between the graphene and a 1 -pyrene butanoic acid succinimidyl ester molecule and an amide bond between the 1- pyrene butanoic acid succinimidyl ester molecule and the detection molecule, and, the detection molecule being an integral membrane receptor molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target. Currently amended
The sensing device of claim 22, the sensing device being configured such that the device, during operation, is capable of detecting a change in signal related to the complementary binding between the detection molecule at the at least one target. Original
The sensing device of claim 22, further comprising a voltage source configured to apply a voltage across the first and second electrodes. Original
A sensing device of claim 22, wherein the detection molecule comprises a protein having an amino acid sequence that is at least about 95 % identical to any one of SEQ ID 1 through SEQ ID
A multiplexed device, comprising: a substrate having a plurality of sensing devices according to claim 22 disposed thereon, at least two of the sensing device comprising different detection molecules. Original
A method of functionalizing a sensing device according to claim 22, comprising: exposing the graphene portion to a solution of carboxylated diazonium salt to create carboxylate groups on the graphene portion; activating the carboxylate groups with a carbodiimide; stabilizing the carboxylate groups with N H S molecules; and displacing the N H S molecules with detection molecules having an accessible amine group so as to create an amide bond between the graphene portion and a receptor molecule. Withdrawn
A method of functionalizing a sensing device according to claim 22, comprising: exposing the graphene portion to Ni-nitrilotriacetic acid molecules having Ni 2+ cations so to create bonds between the nitrilotriacetic acid portion and the graphene; and exposing the Ni 2+ cations to detection molecules having an accessible histidine tag so as to create bonds between the Ni 2+ cations and the detection molecule. Withdrawn
The sensing device of claim 22, wherein the detection molecule comprises protein UniProtK B: P07550 or an associated variant with the capability to bind opioid ligands. Previously presented
Canceled
-29. Canceled
Canceled
-37. Canceled
Canceled
A sensing device, comprising: a graphene portion placing first and second electrodes into electronic communication with one another, the graphene portion comprising a detection molecule bonded thereto, and being characterized as having a D p eak that is less than a G p eak when assessed using Raman spectroscopy, the detection molecule being in electronic communication with the graphene, the detection molecule being a recombinant integral membrane protein molecule comprising a water-soluble variant of a mu receptor capable of complementary binding to at least one target. Currently amended
Layer stacks claimed or described, ordered top of device to substrate.
graphene field effect transistor (GFET) sensor with amide-bonded detection molecule
GFET sensing device with pi-pi stacked 1-PBASE linker and mu receptor detection molecule
Materials described outside the worked examples.
graphene
1-pyrene butanoic acid succinimidyl ester
amide bond linkage
Additional fabrication and treatment steps described in the patent.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
D peak less than G peak (Raman spectroscopy) — graphene quality indicator | — | graphene |
Thickness | 20–100 µm |
Related documents with shared materials, methods, properties, or citations.
multiplexed GFET device with plurality of sensing devices on substrate
GFET sensing device with recombinant integral membrane protein mu receptor
G-protein-coupled-receptor molecule
water-soluble variant of mu receptor (mu-opioid receptor)
opioid analyte
integral membrane receptor molecule
carboxylated diazonium salt
carbodiimide
NHS (N-hydroxysuccinimide)
Ni-nitrilotriacetic acid
PMMA
gold
Au
metal catalyst foil
| — |
Temperature | 10–90 °C | — |
Temperature | 82.9–89.3 °C | — |
Thickness | 205–260 nm | — |
Thickness | 300–350 nm | — |
Thickness | ≥ 15 µm | — |
HIGH YIELD PREPARATION OF MACROSCOPIC GRAPHENE OXIDE MEMBRANES
multiplexed GFET device with plurality of sensing devices on substrate
GFET sensing device with recombinant integral membrane protein mu receptor
G-protein-coupled-receptor molecule
water-soluble variant of mu receptor (mu-opioid receptor)
opioid analyte
integral membrane receptor molecule
carboxylated diazonium salt
carbodiimide
NHS (N-hydroxysuccinimide)
Ni-nitrilotriacetic acid
PMMA
gold
Au
metal catalyst foil
| — |
Temperature | 10–90 °C | — |
Temperature | 82.9–89.3 °C | — |
Thickness | 205–260 nm | — |
Thickness | 300–350 nm | — |
Thickness | ≥ 15 µm | — |
HIGH YIELD PREPARATION OF MACROSCOPIC GRAPHENE OXIDE MEMBRANES
multiplexed GFET device with plurality of sensing devices on substrate
GFET sensing device with recombinant integral membrane protein mu receptor
G-protein-coupled-receptor molecule
water-soluble variant of mu receptor (mu-opioid receptor)
opioid analyte
integral membrane receptor molecule
carboxylated diazonium salt
carbodiimide
NHS (N-hydroxysuccinimide)
Ni-nitrilotriacetic acid
PMMA
gold
Au
metal catalyst foil
| — |
Temperature | 10–90 °C | — |
Temperature | 82.9–89.3 °C | — |
Thickness | 205–260 nm | — |
Thickness | 300–350 nm | — |
Thickness | ≥ 15 µm | — |
HIGH YIELD PREPARATION OF MACROSCOPIC GRAPHENE OXIDE MEMBRANES
multiplexed GFET device with plurality of sensing devices on substrate
GFET sensing device with recombinant integral membrane protein mu receptor
G-protein-coupled-receptor molecule
water-soluble variant of mu receptor (mu-opioid receptor)
opioid analyte
integral membrane receptor molecule
carboxylated diazonium salt
carbodiimide
NHS (N-hydroxysuccinimide)
Ni-nitrilotriacetic acid
PMMA
gold
Au
metal catalyst foil
| — |
Temperature | 10–90 °C | — |
Temperature | 82.9–89.3 °C | — |
Thickness | 205–260 nm | — |
Thickness | 300–350 nm | — |
Thickness | ≥ 15 µm | — |
