Patent
US 8,927,658FLUORESCENT HYBRID OF DENDRIMER AND GRAPHENE OXIDE
Toyoko Imae
US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 8,927,658Patent drawings and their descriptions. Click a drawing to enlarge it.
Figure 1 shows the reaction scheme of OH-terminated PAMAM dendrimer (DEN-OH) and folic acid (FA) bound on graphene oxide (GO) according to one 20 embodiment of the present invention.
Figure 2 shows the UV-vis absorption spectra of GO/DEN-OH/FA hybrids having different concentrations of DEN-OH.
Figures 3A-3E shows fluorescence emission spectra of GO/DEN-OH/FA hybrids at different excitation wavelengths. 3 File: 43783usf
Figure 4 shows two-photon excited fluorescence images of GO/DEN-OH/FA hybrid (DEN-OH = 90 p M) at different excitation wavelengths.
Figure 5 shows the number of fluorescent GO/DEN-OH/FA hybrid upon two-photon excitation at excitation wavelengths of 720 -900 nm. 5
Figure 6 shows microscopic images of HeLa cells with GO/DEN-OH/FA hybrid and of the control before and after laser irradiation.
Figure 7 shows the cell viability as a function of concentration of GO, DEN-OH and GO/DEN-OH/FA hybrid. [0018] Common reference numerals are used throughout the drawings and the 10 detailed description to indicate the same elements. The present invention will be more apparent from the following …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A hybrid material, comprising 50-256 parts by weight of a hydroxyl-terminated poly(amidoamine) dendri m er), 9 parts by weight of folic acid and 11 parts by weight graphene oxide based on a total of the hybrid material, wherein the hydroxyl- terminated poly(amidoamine) dendrimer and the folic acid are each individually and directly bound to and chemically bound on the graphene oxide to form the hybrid material.
The material of claim 1, wherein the hybrid material exhibits two photon absorption p reperty-by showing fluorescence emission of a first wavelength that is obtained at excitation wavelengths [[both]] of a second wavelength and a third wavelength, wherein the second wavelength is shorter than the first wavelength and [[f o af]e third wavelen g th is longer than the first wavelength.
The material of claim 1, wherein the hybrid material absorbs near-infrared light and generates reactive oxygen species (ROS) upon radiation.
The material of claim 1, wherein the hybrid material is water dispersible.
The material of claim 1, wherein the hydroxyl-terminated poly(amidoamine) dendrimer is a fourth-generation hydroxyl-terminated poly(amidoamine) dendrimer. Page 2 of 10 Customer No.: 31561 Docket No.: 43783 -UJ S-PA Application No.: 13/622369
The material of claim 1, wherein the hybrid material functions as a fluorescence marker.
The material of claim 1, wherein the hybrid material functions as an attacking drug in photodynamic therapy.
The material of claim 1, wherein the hybrid material has a relative ratio of the graphene oxide: the hydroxyl-te rm inated poly(amidoamine) dendrimer: the folic acid being l1: 50: 9 by weight for the hybrid material having a concentration of the hydroxyl-te rm inated poly(amidoamine) dendrimer of 18 M.
The material of claim 1, wherein the hybrid material has a relative ratio of the graphene oxide: the hydroxyl-terminated poly(amidoamine) dendrimer: the folic acid being 11: 256: 9 by weight for the hybrid material having a concentration of the hydroxyl-terminated poly(amidoamine) dendrimer of 90 M.
A hybrid material, comprising at least a hydroxyl-te rm inated fourth-generation poly(amidoamine) dendrimer and folic acid each individually and directly bound to and chemically bound on graphene oxide to fo n the hybrid material, wherein the hybrid material is prepared by mixing the hydroxyl-te rn inated poly(amidoamine) dendrimer and the graphene oxide under stirring at a room temperature, adding N,N ' -dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) to a mixture of the hydroxyl-te rm inated poly(amidoamine) dendrimer and the graphene oxide under stirring at the room temperature for esterification to form an intermediate GO/DEN-OH that is the hydroxyl-terminated Page 3 of 10 Customer No,: 31561 Docket No.: 43783-US-PA Application No.: 13/62 2.369 poly(amidoamine) dendrimer bound on the graphene oxide, adding N-hydroxysuccinimide (N HS) and 1-[3- (dimethylamino)propyl]-3-ethyl c arbodiimide hydrochloride (EDC) to an aqueous suspension of the intermediate GO/DEN-OH to form a mixture of N H S, EDC and the aqueous suspension of the intermediate GO/DEN-OH, and then adding the folic acid to the [[a]] mixture of NHS, EDC and the aqueous suspension of the intermediate GO/DEN-OH under stirring at the room temperature to obtain the hybrid material. Page 4 of
Embodiments described in the patent, grouped by the materials and process steps they use.
5 materials1 process step
DMF solution of G-4 OH-terminated PAMAM dendrimer (DEN-OH, 0.2 wt%, 6 cm3) was mixed with DMF solution of GO (10 wt%, 4 cm3) under stirring at room temperature. DCC (3.7–18.5 mg) and DMAP (~1/10 of DCC) were added and the mixture was stirred vigorously at room temperature for 3 days to allow esterification. The dispersion was centrifuged and the centrifugate (GO/DEN-OH) was rinsed with DMF and redispersed in water.
5 materials1 process step
NHS (182.5 mg, 1.6 mmol) and EDC (125 mg, 0.65 mmol) were added to an aqueous suspension (2 cm3) of GO/DEN-OH, and the mixture was ultrasonicated for 2 hours. Then an aqueous 10 wt% NaHCO₃ solution (pH 8) of FA (0.5 wt%, 8 cm3) was added and the mixture was stirred overnight at room temperature. The product (GO/DEN-OH/FA) was purified by dialysis against NaHCO₃ solution (pH 8.0) for 48 hours, then against water for 24 hours.
Materials described outside the worked examples.
folic acid
N,N'-dicyclohexylcarbodiimide (DCC)
Measurements and analyses referenced in the patent, with their drawing references.
Figure 2 shows the UV-vis absorption spectra of GO/DEN-OH/FA hybrids having different concentrations of DEN-OH.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
fluorescence emission peak at 390 nm excitation (one-photon) | 450 nm | GO/DEN-OH/FA hybrid material |
two-photon excited fluorescence emission peak at 780 nm excitation |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 8,927,658Patent drawings and their descriptions. Click a drawing to enlarge it.
Figure 1 shows the reaction scheme of OH-terminated PAMAM dendrimer (DEN-OH) and folic acid (FA) bound on graphene oxide (GO) according to one 20 embodiment of the present invention.
Figure 2 shows the UV-vis absorption spectra of GO/DEN-OH/FA hybrids having different concentrations of DEN-OH.
Figures 3A-3E shows fluorescence emission spectra of GO/DEN-OH/FA hybrids at different excitation wavelengths. 3 File: 43783usf
Figure 4 shows two-photon excited fluorescence images of GO/DEN-OH/FA hybrid (DEN-OH = 90 p M) at different excitation wavelengths.
Figure 5 shows the number of fluorescent GO/DEN-OH/FA hybrid upon two-photon excitation at excitation wavelengths of 720 -900 nm. 5
Figure 6 shows microscopic images of HeLa cells with GO/DEN-OH/FA hybrid and of the control before and after laser irradiation.
Figure 7 shows the cell viability as a function of concentration of GO, DEN-OH and GO/DEN-OH/FA hybrid. [0018] Common reference numerals are used throughout the drawings and the 10 detailed description to indicate the same elements. The present invention will be more apparent from the following …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A hybrid material, comprising 50-256 parts by weight of a hydroxyl-terminated poly(amidoamine) dendri m er), 9 parts by weight of folic acid and 11 parts by weight graphene oxide based on a total of the hybrid material, wherein the hydroxyl- terminated poly(amidoamine) dendrimer and the folic acid are each individually and directly bound to and chemically bound on the graphene oxide to form the hybrid material.
The material of claim 1, wherein the hybrid material exhibits two photon absorption p reperty-by showing fluorescence emission of a first wavelength that is obtained at excitation wavelengths [[both]] of a second wavelength and a third wavelength, wherein the second wavelength is shorter than the first wavelength and [[f o af]e third wavelen g th is longer than the first wavelength.
The material of claim 1, wherein the hybrid material absorbs near-infrared light and generates reactive oxygen species (ROS) upon radiation.
The material of claim 1, wherein the hybrid material is water dispersible.
The material of claim 1, wherein the hydroxyl-terminated poly(amidoamine) dendrimer is a fourth-generation hydroxyl-terminated poly(amidoamine) dendrimer. Page 2 of 10 Customer No.: 31561 Docket No.: 43783 -UJ S-PA Application No.: 13/622369
The material of claim 1, wherein the hybrid material functions as a fluorescence marker.
The material of claim 1, wherein the hybrid material functions as an attacking drug in photodynamic therapy.
The material of claim 1, wherein the hybrid material has a relative ratio of the graphene oxide: the hydroxyl-te rm inated poly(amidoamine) dendrimer: the folic acid being l1: 50: 9 by weight for the hybrid material having a concentration of the hydroxyl-te rm inated poly(amidoamine) dendrimer of 18 M.
The material of claim 1, wherein the hybrid material has a relative ratio of the graphene oxide: the hydroxyl-terminated poly(amidoamine) dendrimer: the folic acid being 11: 256: 9 by weight for the hybrid material having a concentration of the hydroxyl-terminated poly(amidoamine) dendrimer of 90 M.
A hybrid material, comprising at least a hydroxyl-te rm inated fourth-generation poly(amidoamine) dendrimer and folic acid each individually and directly bound to and chemically bound on graphene oxide to fo n the hybrid material, wherein the hybrid material is prepared by mixing the hydroxyl-te rn inated poly(amidoamine) dendrimer and the graphene oxide under stirring at a room temperature, adding N,N ' -dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) to a mixture of the hydroxyl-te rm inated poly(amidoamine) dendrimer and the graphene oxide under stirring at the room temperature for esterification to form an intermediate GO/DEN-OH that is the hydroxyl-terminated Page 3 of 10 Customer No,: 31561 Docket No.: 43783-US-PA Application No.: 13/62 2.369 poly(amidoamine) dendrimer bound on the graphene oxide, adding N-hydroxysuccinimide (N HS) and 1-[3- (dimethylamino)propyl]-3-ethyl c arbodiimide hydrochloride (EDC) to an aqueous suspension of the intermediate GO/DEN-OH to form a mixture of N H S, EDC and the aqueous suspension of the intermediate GO/DEN-OH, and then adding the folic acid to the [[a]] mixture of NHS, EDC and the aqueous suspension of the intermediate GO/DEN-OH under stirring at the room temperature to obtain the hybrid material. Page 4 of
Embodiments described in the patent, grouped by the materials and process steps they use.
5 materials1 process step
DMF solution of G-4 OH-terminated PAMAM dendrimer (DEN-OH, 0.2 wt%, 6 cm3) was mixed with DMF solution of GO (10 wt%, 4 cm3) under stirring at room temperature. DCC (3.7–18.5 mg) and DMAP (~1/10 of DCC) were added and the mixture was stirred vigorously at room temperature for 3 days to allow esterification. The dispersion was centrifuged and the centrifugate (GO/DEN-OH) was rinsed with DMF and redispersed in water.
5 materials1 process step
NHS (182.5 mg, 1.6 mmol) and EDC (125 mg, 0.65 mmol) were added to an aqueous suspension (2 cm3) of GO/DEN-OH, and the mixture was ultrasonicated for 2 hours. Then an aqueous 10 wt% NaHCO₃ solution (pH 8) of FA (0.5 wt%, 8 cm3) was added and the mixture was stirred overnight at room temperature. The product (GO/DEN-OH/FA) was purified by dialysis against NaHCO₃ solution (pH 8.0) for 48 hours, then against water for 24 hours.
Materials described outside the worked examples.
folic acid
N,N'-dicyclohexylcarbodiimide (DCC)
Measurements and analyses referenced in the patent, with their drawing references.
Figure 2 shows the UV-vis absorption spectra of GO/DEN-OH/FA hybrids having different concentrations of DEN-OH.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
fluorescence emission peak at 390 nm excitation (one-photon) | 450 nm | GO/DEN-OH/FA hybrid material |
two-photon excited fluorescence emission peak at 780 nm excitation |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 8,927,658Patent drawings and their descriptions. Click a drawing to enlarge it.
Figure 1 shows the reaction scheme of OH-terminated PAMAM dendrimer (DEN-OH) and folic acid (FA) bound on graphene oxide (GO) according to one 20 embodiment of the present invention.
Figure 2 shows the UV-vis absorption spectra of GO/DEN-OH/FA hybrids having different concentrations of DEN-OH.
Figures 3A-3E shows fluorescence emission spectra of GO/DEN-OH/FA hybrids at different excitation wavelengths. 3 File: 43783usf
Figure 4 shows two-photon excited fluorescence images of GO/DEN-OH/FA hybrid (DEN-OH = 90 p M) at different excitation wavelengths.
Figure 5 shows the number of fluorescent GO/DEN-OH/FA hybrid upon two-photon excitation at excitation wavelengths of 720 -900 nm. 5
Figure 6 shows microscopic images of HeLa cells with GO/DEN-OH/FA hybrid and of the control before and after laser irradiation.
Figure 7 shows the cell viability as a function of concentration of GO, DEN-OH and GO/DEN-OH/FA hybrid. [0018] Common reference numerals are used throughout the drawings and the 10 detailed description to indicate the same elements. The present invention will be more apparent from the following …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A hybrid material, comprising 50-256 parts by weight of a hydroxyl-terminated poly(amidoamine) dendri m er), 9 parts by weight of folic acid and 11 parts by weight graphene oxide based on a total of the hybrid material, wherein the hydroxyl- terminated poly(amidoamine) dendrimer and the folic acid are each individually and directly bound to and chemically bound on the graphene oxide to form the hybrid material.
The material of claim 1, wherein the hybrid material exhibits two photon absorption p reperty-by showing fluorescence emission of a first wavelength that is obtained at excitation wavelengths [[both]] of a second wavelength and a third wavelength, wherein the second wavelength is shorter than the first wavelength and [[f o af]e third wavelen g th is longer than the first wavelength.
The material of claim 1, wherein the hybrid material absorbs near-infrared light and generates reactive oxygen species (ROS) upon radiation.
The material of claim 1, wherein the hybrid material is water dispersible.
The material of claim 1, wherein the hydroxyl-terminated poly(amidoamine) dendrimer is a fourth-generation hydroxyl-terminated poly(amidoamine) dendrimer. Page 2 of 10 Customer No.: 31561 Docket No.: 43783 -UJ S-PA Application No.: 13/622369
The material of claim 1, wherein the hybrid material functions as a fluorescence marker.
The material of claim 1, wherein the hybrid material functions as an attacking drug in photodynamic therapy.
The material of claim 1, wherein the hybrid material has a relative ratio of the graphene oxide: the hydroxyl-te rm inated poly(amidoamine) dendrimer: the folic acid being l1: 50: 9 by weight for the hybrid material having a concentration of the hydroxyl-te rm inated poly(amidoamine) dendrimer of 18 M.
The material of claim 1, wherein the hybrid material has a relative ratio of the graphene oxide: the hydroxyl-terminated poly(amidoamine) dendrimer: the folic acid being 11: 256: 9 by weight for the hybrid material having a concentration of the hydroxyl-terminated poly(amidoamine) dendrimer of 90 M.
A hybrid material, comprising at least a hydroxyl-te rm inated fourth-generation poly(amidoamine) dendrimer and folic acid each individually and directly bound to and chemically bound on graphene oxide to fo n the hybrid material, wherein the hybrid material is prepared by mixing the hydroxyl-te rn inated poly(amidoamine) dendrimer and the graphene oxide under stirring at a room temperature, adding N,N ' -dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) to a mixture of the hydroxyl-te rm inated poly(amidoamine) dendrimer and the graphene oxide under stirring at the room temperature for esterification to form an intermediate GO/DEN-OH that is the hydroxyl-terminated Page 3 of 10 Customer No,: 31561 Docket No.: 43783-US-PA Application No.: 13/62 2.369 poly(amidoamine) dendrimer bound on the graphene oxide, adding N-hydroxysuccinimide (N HS) and 1-[3- (dimethylamino)propyl]-3-ethyl c arbodiimide hydrochloride (EDC) to an aqueous suspension of the intermediate GO/DEN-OH to form a mixture of N H S, EDC and the aqueous suspension of the intermediate GO/DEN-OH, and then adding the folic acid to the [[a]] mixture of NHS, EDC and the aqueous suspension of the intermediate GO/DEN-OH under stirring at the room temperature to obtain the hybrid material. Page 4 of
Embodiments described in the patent, grouped by the materials and process steps they use.
5 materials1 process step
DMF solution of G-4 OH-terminated PAMAM dendrimer (DEN-OH, 0.2 wt%, 6 cm3) was mixed with DMF solution of GO (10 wt%, 4 cm3) under stirring at room temperature. DCC (3.7–18.5 mg) and DMAP (~1/10 of DCC) were added and the mixture was stirred vigorously at room temperature for 3 days to allow esterification. The dispersion was centrifuged and the centrifugate (GO/DEN-OH) was rinsed with DMF and redispersed in water.
5 materials1 process step
NHS (182.5 mg, 1.6 mmol) and EDC (125 mg, 0.65 mmol) were added to an aqueous suspension (2 cm3) of GO/DEN-OH, and the mixture was ultrasonicated for 2 hours. Then an aqueous 10 wt% NaHCO₃ solution (pH 8) of FA (0.5 wt%, 8 cm3) was added and the mixture was stirred overnight at room temperature. The product (GO/DEN-OH/FA) was purified by dialysis against NaHCO₃ solution (pH 8.0) for 48 hours, then against water for 24 hours.
Materials described outside the worked examples.
folic acid
N,N'-dicyclohexylcarbodiimide (DCC)
Measurements and analyses referenced in the patent, with their drawing references.
Figure 2 shows the UV-vis absorption spectra of GO/DEN-OH/FA hybrids having different concentrations of DEN-OH.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
fluorescence emission peak at 390 nm excitation (one-photon) | 450 nm | GO/DEN-OH/FA hybrid material |
two-photon excited fluorescence emission peak at 780 nm excitation |
Related documents with shared materials, methods, properties, or citations.
Patent
Atlas literature
Patent
US 8,927,658Patent drawings and their descriptions. Click a drawing to enlarge it.
Figure 1 shows the reaction scheme of OH-terminated PAMAM dendrimer (DEN-OH) and folic acid (FA) bound on graphene oxide (GO) according to one 20 embodiment of the present invention.
Figure 2 shows the UV-vis absorption spectra of GO/DEN-OH/FA hybrids having different concentrations of DEN-OH.
Figures 3A-3E shows fluorescence emission spectra of GO/DEN-OH/FA hybrids at different excitation wavelengths. 3 File: 43783usf
Figure 4 shows two-photon excited fluorescence images of GO/DEN-OH/FA hybrid (DEN-OH = 90 p M) at different excitation wavelengths.
Figure 5 shows the number of fluorescent GO/DEN-OH/FA hybrid upon two-photon excitation at excitation wavelengths of 720 -900 nm. 5
Figure 6 shows microscopic images of HeLa cells with GO/DEN-OH/FA hybrid and of the control before and after laser irradiation.
Figure 7 shows the cell viability as a function of concentration of GO, DEN-OH and GO/DEN-OH/FA hybrid. [0018] Common reference numerals are used throughout the drawings and the 10 detailed description to indicate the same elements. The present invention will be more apparent from the following …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A hybrid material, comprising 50-256 parts by weight of a hydroxyl-terminated poly(amidoamine) dendri m er), 9 parts by weight of folic acid and 11 parts by weight graphene oxide based on a total of the hybrid material, wherein the hydroxyl- terminated poly(amidoamine) dendrimer and the folic acid are each individually and directly bound to and chemically bound on the graphene oxide to form the hybrid material.
The material of claim 1, wherein the hybrid material exhibits two photon absorption p reperty-by showing fluorescence emission of a first wavelength that is obtained at excitation wavelengths [[both]] of a second wavelength and a third wavelength, wherein the second wavelength is shorter than the first wavelength and [[f o af]e third wavelen g th is longer than the first wavelength.
The material of claim 1, wherein the hybrid material absorbs near-infrared light and generates reactive oxygen species (ROS) upon radiation.
The material of claim 1, wherein the hybrid material is water dispersible.
The material of claim 1, wherein the hydroxyl-terminated poly(amidoamine) dendrimer is a fourth-generation hydroxyl-terminated poly(amidoamine) dendrimer. Page 2 of 10 Customer No.: 31561 Docket No.: 43783 -UJ S-PA Application No.: 13/622369
The material of claim 1, wherein the hybrid material functions as a fluorescence marker.
The material of claim 1, wherein the hybrid material functions as an attacking drug in photodynamic therapy.
The material of claim 1, wherein the hybrid material has a relative ratio of the graphene oxide: the hydroxyl-te rm inated poly(amidoamine) dendrimer: the folic acid being l1: 50: 9 by weight for the hybrid material having a concentration of the hydroxyl-te rm inated poly(amidoamine) dendrimer of 18 M.
The material of claim 1, wherein the hybrid material has a relative ratio of the graphene oxide: the hydroxyl-terminated poly(amidoamine) dendrimer: the folic acid being 11: 256: 9 by weight for the hybrid material having a concentration of the hydroxyl-terminated poly(amidoamine) dendrimer of 90 M.
A hybrid material, comprising at least a hydroxyl-te rm inated fourth-generation poly(amidoamine) dendrimer and folic acid each individually and directly bound to and chemically bound on graphene oxide to fo n the hybrid material, wherein the hybrid material is prepared by mixing the hydroxyl-te rn inated poly(amidoamine) dendrimer and the graphene oxide under stirring at a room temperature, adding N,N ' -dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) to a mixture of the hydroxyl-te rm inated poly(amidoamine) dendrimer and the graphene oxide under stirring at the room temperature for esterification to form an intermediate GO/DEN-OH that is the hydroxyl-terminated Page 3 of 10 Customer No,: 31561 Docket No.: 43783-US-PA Application No.: 13/62 2.369 poly(amidoamine) dendrimer bound on the graphene oxide, adding N-hydroxysuccinimide (N HS) and 1-[3- (dimethylamino)propyl]-3-ethyl c arbodiimide hydrochloride (EDC) to an aqueous suspension of the intermediate GO/DEN-OH to form a mixture of N H S, EDC and the aqueous suspension of the intermediate GO/DEN-OH, and then adding the folic acid to the [[a]] mixture of NHS, EDC and the aqueous suspension of the intermediate GO/DEN-OH under stirring at the room temperature to obtain the hybrid material. Page 4 of
Embodiments described in the patent, grouped by the materials and process steps they use.
5 materials1 process step
DMF solution of G-4 OH-terminated PAMAM dendrimer (DEN-OH, 0.2 wt%, 6 cm3) was mixed with DMF solution of GO (10 wt%, 4 cm3) under stirring at room temperature. DCC (3.7–18.5 mg) and DMAP (~1/10 of DCC) were added and the mixture was stirred vigorously at room temperature for 3 days to allow esterification. The dispersion was centrifuged and the centrifugate (GO/DEN-OH) was rinsed with DMF and redispersed in water.
5 materials1 process step
NHS (182.5 mg, 1.6 mmol) and EDC (125 mg, 0.65 mmol) were added to an aqueous suspension (2 cm3) of GO/DEN-OH, and the mixture was ultrasonicated for 2 hours. Then an aqueous 10 wt% NaHCO₃ solution (pH 8) of FA (0.5 wt%, 8 cm3) was added and the mixture was stirred overnight at room temperature. The product (GO/DEN-OH/FA) was purified by dialysis against NaHCO₃ solution (pH 8.0) for 48 hours, then against water for 24 hours.
Materials described outside the worked examples.
folic acid
N,N'-dicyclohexylcarbodiimide (DCC)
Measurements and analyses referenced in the patent, with their drawing references.
Figure 2 shows the UV-vis absorption spectra of GO/DEN-OH/FA hybrids having different concentrations of DEN-OH.
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
fluorescence emission peak at 390 nm excitation (one-photon) | 450 nm | GO/DEN-OH/FA hybrid material |
two-photon excited fluorescence emission peak at 780 nm excitation |
Related documents with shared materials, methods, properties, or citations.
| 450 nm |
GO/DEN-OH/FA hybrid material |
UV-vis absorption band after FA loading onto GO/DEN-OH | 280 nm | GO/DEN-OH/FA hybrid material |
fluorescence emission band at 230 nm excitation | 355 nm | GO/DEN-OH/FA hybrid material |
Pressure | 50–256 pa | — |
Thickness | 720–990 nm | — |
Thickness | 760–920 nm | — |
Thickness | 700–1100 nm | — |
GRAPHENE BACKLIGHT MODULE AND LIQUID CYSTAL DISPLAY DEVICE INCLUDING THE SAME
| 450 nm |
GO/DEN-OH/FA hybrid material |
UV-vis absorption band after FA loading onto GO/DEN-OH | 280 nm | GO/DEN-OH/FA hybrid material |
fluorescence emission band at 230 nm excitation | 355 nm | GO/DEN-OH/FA hybrid material |
Pressure | 50–256 pa | — |
Thickness | 720–990 nm | — |
Thickness | 760–920 nm | — |
Thickness | 700–1100 nm | — |
GRAPHENE BACKLIGHT MODULE AND LIQUID CYSTAL DISPLAY DEVICE INCLUDING THE SAME
| 450 nm |
GO/DEN-OH/FA hybrid material |
UV-vis absorption band after FA loading onto GO/DEN-OH | 280 nm | GO/DEN-OH/FA hybrid material |
fluorescence emission band at 230 nm excitation | 355 nm | GO/DEN-OH/FA hybrid material |
Pressure | 50–256 pa | — |
Thickness | 720–990 nm | — |
Thickness | 760–920 nm | — |
Thickness | 700–1100 nm | — |
GRAPHENE BACKLIGHT MODULE AND LIQUID CYSTAL DISPLAY DEVICE INCLUDING THE SAME
| 450 nm |
GO/DEN-OH/FA hybrid material |
UV-vis absorption band after FA loading onto GO/DEN-OH | 280 nm | GO/DEN-OH/FA hybrid material |
fluorescence emission band at 230 nm excitation | 355 nm | GO/DEN-OH/FA hybrid material |
Pressure | 50–256 pa | — |
Thickness | 720–990 nm | — |
Thickness | 760–920 nm | — |
Thickness | 700–1100 nm | — |
GRAPHENE BACKLIGHT MODULE AND LIQUID CYSTAL DISPLAY DEVICE INCLUDING THE SAME