Patent
US 10,465,244TITLE: A NOVEL ELECTROCHEMICAL DNA BIOSENSOR USING GRAPHENE BIOCHIP FOR SPECIES IDENTIFICATION
Sharmili Roy, Ibrahim Abdul Rahman, Minhaz Uddin Ahmed
US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 10,465,244Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 D). [017] Fig. 2 shows scheme of SWV in higher cathodic peak current in presence of pork specific isothermal amplicon (LAMP product) (d), compared to …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
Canceled
Canceled
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Canceled
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Canceled
Canceled
A method of using a graphene biosensor to detect a specific species in a food item, comprising: synthesizing a primer specific for the specific species to be tested in fer the food item; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution on the surface of graphene biosensor; contacting the graphene biosensor with the food item to be tested; allowing the binding of a DNA from of the species in the food item to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer; and detecting the cathodic current i-s produced by applying voltage by means of square wave voltammetry, such that a higher catholic current is produced indicating presence of DNA in the food item in comparison to the low catholic current indicating an absence of DNA in the food item. 3 Currently amended
(Cu rr ently Amended) The method of claim 8, wherein the ruthenium hexamine binds with the free DNA present in the food item indicating presence of specific species in the food item. Currently amended
The method of claim 8, wherein the DNA is detected in picogram level in the food item. Currently amended
Canceled
Canceled
Canceled
(Cu rr ently Amended) A method of using a graphene biosensor to detect a meat species in a food item, comprising: synthesizing a primer for the meat species to be detected; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution ont e the surface of graphene biosensor; contacting the graphene biosensor with the food item to be tested; allowing the binding of te -a DNA from of the meat species to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer; and detecting the cathodic current produced by applying voltage by means of square wave voltammetry, such that a higher catholic current is produced indicating presence of DNA of the meat species in the food item in comparison to the low catholic current indicating an absence of DNA of the meat species in the food item. 4 Currently amended
(Cu rr ently Amended) The method of claim 14, wherein presence of the DNA is detected a t picogram level in the food item. Currently amended
Canceled
Canceled
A method of using a graphene biosensor to detect more than one species in a food item, comprising: synthesizing a primer specific for each species to be tested in the food item; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution on the surface of graphene biosensor; diluting the food item from 1000 pg to l pg; contacting the graphene biosensor with the food item; allowing the binding of DNA from each species in the food item to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer specific for each species to be tested; and detecting the cathodic current produced by applying voltage that a higher catholic current is produced indicating comparison to the low catholic current indicating
The method of claim 18, wherein primers are the food item. New
The method of claim 18, wherein the species New
. The method of claim 18, wherein presence food item. New by means of square wave voltammetry, such presence of DNA in the food item in an absence of DNA in the food item. New synthesized for every species to be detected in maybe one of meat, pork, chicken, or salmon. of the DNA is detected at picogram level in the
Layer stacks claimed or described, ordered top of device to substrate.
graphene biosensor/graphene screen-printed electrode (biochip)
Materials described outside the worked examples.
graphene
ruthenium hexamine
[Ru(NH₃)6]3+
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
DNA detection limit in food item | 1 pg/µL to 100 pg/µL pg/µL | — |
Temperature | 23–25 °C | — |
Patent
Atlas literature
Patent
US 10,465,244Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 D). [017] Fig. 2 shows scheme of SWV in higher cathodic peak current in presence of pork specific isothermal amplicon (LAMP product) (d), compared to …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
Canceled
Canceled
Canceled
Canceled
Canceled
Canceled
Canceled
A method of using a graphene biosensor to detect a specific species in a food item, comprising: synthesizing a primer specific for the specific species to be tested in fer the food item; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution on the surface of graphene biosensor; contacting the graphene biosensor with the food item to be tested; allowing the binding of a DNA from of the species in the food item to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer; and detecting the cathodic current i-s produced by applying voltage by means of square wave voltammetry, such that a higher catholic current is produced indicating presence of DNA in the food item in comparison to the low catholic current indicating an absence of DNA in the food item. 3 Currently amended
(Cu rr ently Amended) The method of claim 8, wherein the ruthenium hexamine binds with the free DNA present in the food item indicating presence of specific species in the food item. Currently amended
The method of claim 8, wherein the DNA is detected in picogram level in the food item. Currently amended
Canceled
Canceled
Canceled
(Cu rr ently Amended) A method of using a graphene biosensor to detect a meat species in a food item, comprising: synthesizing a primer for the meat species to be detected; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution ont e the surface of graphene biosensor; contacting the graphene biosensor with the food item to be tested; allowing the binding of te -a DNA from of the meat species to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer; and detecting the cathodic current produced by applying voltage by means of square wave voltammetry, such that a higher catholic current is produced indicating presence of DNA of the meat species in the food item in comparison to the low catholic current indicating an absence of DNA of the meat species in the food item. 4 Currently amended
(Cu rr ently Amended) The method of claim 14, wherein presence of the DNA is detected a t picogram level in the food item. Currently amended
Canceled
Canceled
A method of using a graphene biosensor to detect more than one species in a food item, comprising: synthesizing a primer specific for each species to be tested in the food item; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution on the surface of graphene biosensor; diluting the food item from 1000 pg to l pg; contacting the graphene biosensor with the food item; allowing the binding of DNA from each species in the food item to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer specific for each species to be tested; and detecting the cathodic current produced by applying voltage that a higher catholic current is produced indicating comparison to the low catholic current indicating
The method of claim 18, wherein primers are the food item. New
The method of claim 18, wherein the species New
. The method of claim 18, wherein presence food item. New by means of square wave voltammetry, such presence of DNA in the food item in an absence of DNA in the food item. New synthesized for every species to be detected in maybe one of meat, pork, chicken, or salmon. of the DNA is detected at picogram level in the
Layer stacks claimed or described, ordered top of device to substrate.
graphene biosensor/graphene screen-printed electrode (biochip)
Materials described outside the worked examples.
graphene
ruthenium hexamine
[Ru(NH₃)6]3+
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
DNA detection limit in food item | 1 pg/µL to 100 pg/µL pg/µL | — |
Temperature | 23–25 °C | — |
Patent
Atlas literature
Patent
US 10,465,244Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 D). [017] Fig. 2 shows scheme of SWV in higher cathodic peak current in presence of pork specific isothermal amplicon (LAMP product) (d), compared to …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
Canceled
Canceled
Canceled
Canceled
Canceled
Canceled
Canceled
A method of using a graphene biosensor to detect a specific species in a food item, comprising: synthesizing a primer specific for the specific species to be tested in fer the food item; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution on the surface of graphene biosensor; contacting the graphene biosensor with the food item to be tested; allowing the binding of a DNA from of the species in the food item to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer; and detecting the cathodic current i-s produced by applying voltage by means of square wave voltammetry, such that a higher catholic current is produced indicating presence of DNA in the food item in comparison to the low catholic current indicating an absence of DNA in the food item. 3 Currently amended
(Cu rr ently Amended) The method of claim 8, wherein the ruthenium hexamine binds with the free DNA present in the food item indicating presence of specific species in the food item. Currently amended
The method of claim 8, wherein the DNA is detected in picogram level in the food item. Currently amended
Canceled
Canceled
Canceled
(Cu rr ently Amended) A method of using a graphene biosensor to detect a meat species in a food item, comprising: synthesizing a primer for the meat species to be detected; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution ont e the surface of graphene biosensor; contacting the graphene biosensor with the food item to be tested; allowing the binding of te -a DNA from of the meat species to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer; and detecting the cathodic current produced by applying voltage by means of square wave voltammetry, such that a higher catholic current is produced indicating presence of DNA of the meat species in the food item in comparison to the low catholic current indicating an absence of DNA of the meat species in the food item. 4 Currently amended
(Cu rr ently Amended) The method of claim 14, wherein presence of the DNA is detected a t picogram level in the food item. Currently amended
Canceled
Canceled
A method of using a graphene biosensor to detect more than one species in a food item, comprising: synthesizing a primer specific for each species to be tested in the food item; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution on the surface of graphene biosensor; diluting the food item from 1000 pg to l pg; contacting the graphene biosensor with the food item; allowing the binding of DNA from each species in the food item to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer specific for each species to be tested; and detecting the cathodic current produced by applying voltage that a higher catholic current is produced indicating comparison to the low catholic current indicating
The method of claim 18, wherein primers are the food item. New
The method of claim 18, wherein the species New
. The method of claim 18, wherein presence food item. New by means of square wave voltammetry, such presence of DNA in the food item in an absence of DNA in the food item. New synthesized for every species to be detected in maybe one of meat, pork, chicken, or salmon. of the DNA is detected at picogram level in the
Layer stacks claimed or described, ordered top of device to substrate.
graphene biosensor/graphene screen-printed electrode (biochip)
Materials described outside the worked examples.
graphene
ruthenium hexamine
[Ru(NH₃)6]3+
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
DNA detection limit in food item | 1 pg/µL to 100 pg/µL pg/µL | — |
Temperature | 23–25 °C | — |
Patent
Atlas literature
Patent
US 10,465,244Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 D). [017] Fig. 2 shows scheme of SWV in higher cathodic peak current in presence of pork specific isothermal amplicon (LAMP product) (d), compared to …
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
Canceled
Canceled
Canceled
Canceled
Canceled
Canceled
Canceled
A method of using a graphene biosensor to detect a specific species in a food item, comprising: synthesizing a primer specific for the specific species to be tested in fer the food item; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution on the surface of graphene biosensor; contacting the graphene biosensor with the food item to be tested; allowing the binding of a DNA from of the species in the food item to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer; and detecting the cathodic current i-s produced by applying voltage by means of square wave voltammetry, such that a higher catholic current is produced indicating presence of DNA in the food item in comparison to the low catholic current indicating an absence of DNA in the food item. 3 Currently amended
(Cu rr ently Amended) The method of claim 8, wherein the ruthenium hexamine binds with the free DNA present in the food item indicating presence of specific species in the food item. Currently amended
The method of claim 8, wherein the DNA is detected in picogram level in the food item. Currently amended
Canceled
Canceled
Canceled
(Cu rr ently Amended) A method of using a graphene biosensor to detect a meat species in a food item, comprising: synthesizing a primer for the meat species to be detected; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution ont e the surface of graphene biosensor; contacting the graphene biosensor with the food item to be tested; allowing the binding of te -a DNA from of the meat species to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer; and detecting the cathodic current produced by applying voltage by means of square wave voltammetry, such that a higher catholic current is produced indicating presence of DNA of the meat species in the food item in comparison to the low catholic current indicating an absence of DNA of the meat species in the food item. 4 Currently amended
(Cu rr ently Amended) The method of claim 14, wherein presence of the DNA is detected a t picogram level in the food item. Currently amended
Canceled
Canceled
A method of using a graphene biosensor to detect more than one species in a food item, comprising: synthesizing a primer specific for each species to be tested in the food item; preparing a ruthenium hexamine solution; adsorbing the ruthenium hexamine solution on the surface of graphene biosensor; diluting the food item from 1000 pg to l pg; contacting the graphene biosensor with the food item; allowing the binding of DNA from each species in the food item to the ruthenium hexamine solution; amplifying the bound DNA using loop mediated isothermal amplification method using synthesized primer specific for each species to be tested; and detecting the cathodic current produced by applying voltage that a higher catholic current is produced indicating comparison to the low catholic current indicating
The method of claim 18, wherein primers are the food item. New
The method of claim 18, wherein the species New
. The method of claim 18, wherein presence food item. New by means of square wave voltammetry, such presence of DNA in the food item in an absence of DNA in the food item. New synthesized for every species to be detected in maybe one of meat, pork, chicken, or salmon. of the DNA is detected at picogram level in the
Layer stacks claimed or described, ordered top of device to substrate.
graphene biosensor/graphene screen-printed electrode (biochip)
Materials described outside the worked examples.
graphene
ruthenium hexamine
[Ru(NH₃)6]3+
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
DNA detection limit in food item | 1 pg/µL to 100 pg/µL pg/µL | — |
Temperature | 23–25 °C | — |