Patent
US 10,935,550SYSTEM FOR DETECTING RARE CELLS
NAGRATH, Sunitha, YOON, Hyeun Joong
THE REGENTS OF THE UNIVERSITY OF MICHIGAN·4 April 2013·US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 10,935,550Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A system for detecting cells, said system comprising: A. a substrate and one or more supplemental layers disposed on said substrate, wherein said one or more supplemental layers have a height ranging from 0.1 millimeters to 100 [[inches]]millimeters; B. an extension coupled to one of said one or more supplemental layers and extending outwardly from said substrate; and C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the cells to immobilize the cells on said extension when the cells come into contact with said functionalized graphene oxide. Currently amended
The system of claim 1 wherein said functionalized graphene oxide has a thickness of from 1 to 5 nanometers. Original
The system of claim 1 wherein said binding agent comprises phospholipid-polyethylene-glyco-amine. Previously presented
The system of claim 1 wherein said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N-y- maleimidobutyryloxy succinimide ester. Currently amended
The system of claim 1 wherein said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N- y- maleimidobutyryloxy succinimide ester, wherein said reaction product is further bonded to a protein, and wherein said protein is further bonded to an antibody for interaction with the cells. Currently amended
The system of claim 1 that is further defined as a microfluidic device. Previously presented
The system of claim 1, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
The system of claim 1, wherein the cells comprise one or more of circulating tumor cells, endothelial cells, fetal cells, and cells of hemopoetic origin. Previously presented
The system of claim 1, wherein the extension has a height of from 1 to 500 nanometers. Previously presented
Canceled
1 1-13. Canceled
Canceled
A microfluidic device for detecting [[rare]]cells, said device comprising: A. a silicon substrate and one or more supplemental layers disposed on said substrate, wherein said one or more supplemental layers have a height ranging from 0.1 millimeters to 100 [[inches]] millimeters; B. an extension that is a metal, that is coupled to one of said one or more supplemental layers, and that extends outwardly from said substrate; and C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the cells to immobilize the cells on said extension when the cells come into contact with said functionalized graphene oxide. Currently amended
The microfluidic device of claim 16 wherein said metal is gold, said graphene oxide is functionalized with a binding agent, said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N- y -maleimidobutyryloxy succinimide ester, said reaction product is further bonded to a protein, and said protein is further bonded to an antibody for interaction with the cells. Currently amended
The microfluidic device of claim 16, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
A method for capturing cells using a system comprising a substrate and one or more supplemental layers disposed on said substrate, an extension coupled to one of said one or more supplemental layers and extending outwardly from said substrate with the one or more supplemental layers having a height of from 0.1 millimeters to 100 [[inches]]millimeters, and a functionalized graphene oxide disposed on the extension, said method comprising the steps of: I. providing the system; II. introducing a sample of bodily fluid containing the cells into the system; and III. immobilizing the cells on the extension as the cells interact with the functionalized graphene oxide when the cells come into contact with the functionalized graphene oxide. Currently amended
The method of claim 18 wherein the functionalized graphene oxide has a thickness of from 1 to 5 nanometers. Previously presented
The method of claim 18 wherein the extension is gold. Previously presented
The method of claim 18, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
Layer stacks claimed or described, ordered top of device to substrate.
cell detection system
microfluidic device for cell detection
Materials described outside the worked examples.
functionalized graphene oxide
gold
Au
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Thickness | 1–1.5 nm | — |
Thickness |
Patent
Atlas literature
Patent
US 10,935,550Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A system for detecting cells, said system comprising: A. a substrate and one or more supplemental layers disposed on said substrate, wherein said one or more supplemental layers have a height ranging from 0.1 millimeters to 100 [[inches]]millimeters; B. an extension coupled to one of said one or more supplemental layers and extending outwardly from said substrate; and C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the cells to immobilize the cells on said extension when the cells come into contact with said functionalized graphene oxide. Currently amended
The system of claim 1 wherein said functionalized graphene oxide has a thickness of from 1 to 5 nanometers. Original
The system of claim 1 wherein said binding agent comprises phospholipid-polyethylene-glyco-amine. Previously presented
The system of claim 1 wherein said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N-y- maleimidobutyryloxy succinimide ester. Currently amended
The system of claim 1 wherein said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N- y- maleimidobutyryloxy succinimide ester, wherein said reaction product is further bonded to a protein, and wherein said protein is further bonded to an antibody for interaction with the cells. Currently amended
The system of claim 1 that is further defined as a microfluidic device. Previously presented
The system of claim 1, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
The system of claim 1, wherein the cells comprise one or more of circulating tumor cells, endothelial cells, fetal cells, and cells of hemopoetic origin. Previously presented
The system of claim 1, wherein the extension has a height of from 1 to 500 nanometers. Previously presented
Canceled
1 1-13. Canceled
Canceled
A microfluidic device for detecting [[rare]]cells, said device comprising: A. a silicon substrate and one or more supplemental layers disposed on said substrate, wherein said one or more supplemental layers have a height ranging from 0.1 millimeters to 100 [[inches]] millimeters; B. an extension that is a metal, that is coupled to one of said one or more supplemental layers, and that extends outwardly from said substrate; and C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the cells to immobilize the cells on said extension when the cells come into contact with said functionalized graphene oxide. Currently amended
The microfluidic device of claim 16 wherein said metal is gold, said graphene oxide is functionalized with a binding agent, said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N- y -maleimidobutyryloxy succinimide ester, said reaction product is further bonded to a protein, and said protein is further bonded to an antibody for interaction with the cells. Currently amended
The microfluidic device of claim 16, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
A method for capturing cells using a system comprising a substrate and one or more supplemental layers disposed on said substrate, an extension coupled to one of said one or more supplemental layers and extending outwardly from said substrate with the one or more supplemental layers having a height of from 0.1 millimeters to 100 [[inches]]millimeters, and a functionalized graphene oxide disposed on the extension, said method comprising the steps of: I. providing the system; II. introducing a sample of bodily fluid containing the cells into the system; and III. immobilizing the cells on the extension as the cells interact with the functionalized graphene oxide when the cells come into contact with the functionalized graphene oxide. Currently amended
The method of claim 18 wherein the functionalized graphene oxide has a thickness of from 1 to 5 nanometers. Previously presented
The method of claim 18 wherein the extension is gold. Previously presented
The method of claim 18, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
Layer stacks claimed or described, ordered top of device to substrate.
cell detection system
microfluidic device for cell detection
Materials described outside the worked examples.
functionalized graphene oxide
gold
Au
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Thickness | 1–1.5 nm | — |
Thickness |
Patent
Atlas literature
Patent
US 10,935,550Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A system for detecting cells, said system comprising: A. a substrate and one or more supplemental layers disposed on said substrate, wherein said one or more supplemental layers have a height ranging from 0.1 millimeters to 100 [[inches]]millimeters; B. an extension coupled to one of said one or more supplemental layers and extending outwardly from said substrate; and C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the cells to immobilize the cells on said extension when the cells come into contact with said functionalized graphene oxide. Currently amended
The system of claim 1 wherein said functionalized graphene oxide has a thickness of from 1 to 5 nanometers. Original
The system of claim 1 wherein said binding agent comprises phospholipid-polyethylene-glyco-amine. Previously presented
The system of claim 1 wherein said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N-y- maleimidobutyryloxy succinimide ester. Currently amended
The system of claim 1 wherein said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N- y- maleimidobutyryloxy succinimide ester, wherein said reaction product is further bonded to a protein, and wherein said protein is further bonded to an antibody for interaction with the cells. Currently amended
The system of claim 1 that is further defined as a microfluidic device. Previously presented
The system of claim 1, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
The system of claim 1, wherein the cells comprise one or more of circulating tumor cells, endothelial cells, fetal cells, and cells of hemopoetic origin. Previously presented
The system of claim 1, wherein the extension has a height of from 1 to 500 nanometers. Previously presented
Canceled
1 1-13. Canceled
Canceled
A microfluidic device for detecting [[rare]]cells, said device comprising: A. a silicon substrate and one or more supplemental layers disposed on said substrate, wherein said one or more supplemental layers have a height ranging from 0.1 millimeters to 100 [[inches]] millimeters; B. an extension that is a metal, that is coupled to one of said one or more supplemental layers, and that extends outwardly from said substrate; and C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the cells to immobilize the cells on said extension when the cells come into contact with said functionalized graphene oxide. Currently amended
The microfluidic device of claim 16 wherein said metal is gold, said graphene oxide is functionalized with a binding agent, said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N- y -maleimidobutyryloxy succinimide ester, said reaction product is further bonded to a protein, and said protein is further bonded to an antibody for interaction with the cells. Currently amended
The microfluidic device of claim 16, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
A method for capturing cells using a system comprising a substrate and one or more supplemental layers disposed on said substrate, an extension coupled to one of said one or more supplemental layers and extending outwardly from said substrate with the one or more supplemental layers having a height of from 0.1 millimeters to 100 [[inches]]millimeters, and a functionalized graphene oxide disposed on the extension, said method comprising the steps of: I. providing the system; II. introducing a sample of bodily fluid containing the cells into the system; and III. immobilizing the cells on the extension as the cells interact with the functionalized graphene oxide when the cells come into contact with the functionalized graphene oxide. Currently amended
The method of claim 18 wherein the functionalized graphene oxide has a thickness of from 1 to 5 nanometers. Previously presented
The method of claim 18 wherein the extension is gold. Previously presented
The method of claim 18, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
Layer stacks claimed or described, ordered top of device to substrate.
cell detection system
microfluidic device for cell detection
Materials described outside the worked examples.
functionalized graphene oxide
gold
Au
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Thickness | 1–1.5 nm | — |
Thickness |
Patent
Atlas literature
Patent
US 10,935,550Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A system for detecting cells, said system comprising: A. a substrate and one or more supplemental layers disposed on said substrate, wherein said one or more supplemental layers have a height ranging from 0.1 millimeters to 100 [[inches]]millimeters; B. an extension coupled to one of said one or more supplemental layers and extending outwardly from said substrate; and C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the cells to immobilize the cells on said extension when the cells come into contact with said functionalized graphene oxide. Currently amended
The system of claim 1 wherein said functionalized graphene oxide has a thickness of from 1 to 5 nanometers. Original
The system of claim 1 wherein said binding agent comprises phospholipid-polyethylene-glyco-amine. Previously presented
The system of claim 1 wherein said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N-y- maleimidobutyryloxy succinimide ester. Currently amended
The system of claim 1 wherein said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N- y- maleimidobutyryloxy succinimide ester, wherein said reaction product is further bonded to a protein, and wherein said protein is further bonded to an antibody for interaction with the cells. Currently amended
The system of claim 1 that is further defined as a microfluidic device. Previously presented
The system of claim 1, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
The system of claim 1, wherein the cells comprise one or more of circulating tumor cells, endothelial cells, fetal cells, and cells of hemopoetic origin. Previously presented
The system of claim 1, wherein the extension has a height of from 1 to 500 nanometers. Previously presented
Canceled
1 1-13. Canceled
Canceled
A microfluidic device for detecting [[rare]]cells, said device comprising: A. a silicon substrate and one or more supplemental layers disposed on said substrate, wherein said one or more supplemental layers have a height ranging from 0.1 millimeters to 100 [[inches]] millimeters; B. an extension that is a metal, that is coupled to one of said one or more supplemental layers, and that extends outwardly from said substrate; and C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the cells to immobilize the cells on said extension when the cells come into contact with said functionalized graphene oxide. Currently amended
The microfluidic device of claim 16 wherein said metal is gold, said graphene oxide is functionalized with a binding agent, said binding agent comprises [[the]]a reaction product of phospholipid-polyethylene-glyco-amine and N- y -maleimidobutyryloxy succinimide ester, said reaction product is further bonded to a protein, and said protein is further bonded to an antibody for interaction with the cells. Currently amended
The microfluidic device of claim 16, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
A method for capturing cells using a system comprising a substrate and one or more supplemental layers disposed on said substrate, an extension coupled to one of said one or more supplemental layers and extending outwardly from said substrate with the one or more supplemental layers having a height of from 0.1 millimeters to 100 [[inches]]millimeters, and a functionalized graphene oxide disposed on the extension, said method comprising the steps of: I. providing the system; II. introducing a sample of bodily fluid containing the cells into the system; and III. immobilizing the cells on the extension as the cells interact with the functionalized graphene oxide when the cells come into contact with the functionalized graphene oxide. Currently amended
The method of claim 18 wherein the functionalized graphene oxide has a thickness of from 1 to 5 nanometers. Previously presented
The method of claim 18 wherein the extension is gold. Previously presented
The method of claim 18, wherein at least one of said one or more supplemental layers is selected from the group consisting of a polydimethylsiloxane layer, a polyethylene terephthalate layer, a polyimide layer, and a polyether ether ketone layer. Previously presented
Layer stacks claimed or described, ordered top of device to substrate.
cell detection system
microfluidic device for cell detection
Materials described outside the worked examples.
functionalized graphene oxide
gold
Au
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Thickness | 1–1.5 nm | — |
Thickness |
N-gamma-maleimidobutyryloxy succinimide ester
deglycosylated avidin
anti-EpCAM antibody
silicon
Si
polydimethylsiloxane
polyethylene terephthalate
polyimide
polyether ether ketone
| — |
Thickness | 100–1000000 nm | — |
Thickness | 100–1000 nm | — |
Thickness | 100–1000 mm | — |
Thickness | 5–100 mm | — |
Thickness | 5–50 mm | — |
Thickness | 40–50 µm | — |
Thickness | 1–5 nm | — |
Thickness | 1–500 nm | — |
N-gamma-maleimidobutyryloxy succinimide ester
deglycosylated avidin
anti-EpCAM antibody
silicon
Si
polydimethylsiloxane
polyethylene terephthalate
polyimide
polyether ether ketone
| — |
Thickness | 100–1000000 nm | — |
Thickness | 100–1000 nm | — |
Thickness | 100–1000 mm | — |
Thickness | 5–100 mm | — |
Thickness | 5–50 mm | — |
Thickness | 40–50 µm | — |
Thickness | 1–5 nm | — |
Thickness | 1–500 nm | — |
N-gamma-maleimidobutyryloxy succinimide ester
deglycosylated avidin
anti-EpCAM antibody
silicon
Si
polydimethylsiloxane
polyethylene terephthalate
polyimide
polyether ether ketone
| — |
Thickness | 100–1000000 nm | — |
Thickness | 100–1000 nm | — |
Thickness | 100–1000 mm | — |
Thickness | 5–100 mm | — |
Thickness | 5–50 mm | — |
Thickness | 40–50 µm | — |
Thickness | 1–5 nm | — |
Thickness | 1–500 nm | — |
N-gamma-maleimidobutyryloxy succinimide ester
deglycosylated avidin
anti-EpCAM antibody
silicon
Si
polydimethylsiloxane
polyethylene terephthalate
polyimide
polyether ether ketone
| — |
Thickness | 100–1000000 nm | — |
Thickness | 100–1000 nm | — |
Thickness | 100–1000 mm | — |
Thickness | 5–100 mm | — |
Thickness | 5–50 mm | — |
Thickness | 40–50 µm | — |
Thickness | 1–5 nm | — |
Thickness | 1–500 nm | — |