Patent
US 10,114,024BIOMOLECULE-GRAPHENE QUANTUM DOT CONJUGATES AND USE THEREOF
Peng Chen
US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 10,114,024Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method to form an insulin-G Q D conjugate, wherein a single biomolecule of insulin is coupled to a single graphene quantum dot (G Q D), the method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the GQD comprises one or more carboxyl groups on an edge of the GQD; and wherein 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride reacts with the one or more carboxyl groups to form an O-acylisourea intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), to provide an N H S ester-modified GQD; and contacting the N H S ester-modified G Q D with biomolecules of insulin to form the insulin -GQD conjugat,,,,,, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell. Currently amended
The method of claim 1, further comprising contacting the insulin -GQD conjugate with hydroxylamine solution. Currently amended
1 5. The method of claim 1 wherein forming N H S to the one or more carboxyl groups by ester
The method of claim 1 wherein forming the amide bond between a n amine of the biomolecule Currently amended optical imaging is confocal fluorescence subject is a mammal. Withdrawn part of the subject is a mammalian adipocyte the N HS ester-modified GQD comprises coupling bonds. Previously presented insulin -GQD conjugate comprises forming an of insulin and a carboxyl group of GQD.
3-5. Canceled
Canceled
A fluorophore comprising a n insulin-GQD conjugate, wherein a single biomolecule of insulin is coupled to a single GQD, obtained by a method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride reacts with one or more carboxyl groups present on an edge of the GQD to form an O -acylisour e a intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), wherein the O-acylisourea intermediate compound reacts with the N-hydroxysucci ni mide (NH S) to form a N H S ester-modified GQD; and contacting the N H S ester-modified GQD with biomolecules of insulin to form o an am n ea row ac or, su, u,,,, the insulin -GQD conjugate, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell. Currently amended
7-8. Canceled
Canceled
(Withdrawn-Currently Amended) An imaging method, comprising: administering a fluorophore to a subject, wherein the fluorophore comprises an insulin -GQD conjugate, wherein a single biomolecule of insulin is coupled to a single GQD, obtained by a method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the 1-ethyl-3-(3-dimethylami n opropyl) carbodiimide hydrochloride reacts with one or more carboxyl groups present on an edge of the GQD to form an O-acylisourea intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), wherein the O-acylisourea intermediate compound reacts with the N-hydroxysuccini m ide (NH S) to form a N H S ester-modified GQD; and contacting the N H S ester-modified GQD with biomolecules of insulin to form,,,,,, the insulin -GQD conjugate, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell; and collecting imaging data of the subject or part of the subject with an optical imaging. Currently amended
1 0. The imaging method of claim 9, wherein the optical imaging comprises optical multimodality imaging. Withdrawn
The imaging method of claim 9, wherein the optical imaging is ex vivo imaging. Withdrawn
The imaging method of claim 9, wherein the imaging. Withdrawn
The imaging method of claim 9, wherein the
Layer stacks claimed or described, ordered top of device to substrate.
insulin-GQD fluorophore
Materials described outside the worked examples.
graphene quantum dot (GQD)
insulin
Additional fabrication and treatment steps described in the patent.
Patent
Atlas literature
Patent
US 10,114,024Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method to form an insulin-G Q D conjugate, wherein a single biomolecule of insulin is coupled to a single graphene quantum dot (G Q D), the method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the GQD comprises one or more carboxyl groups on an edge of the GQD; and wherein 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride reacts with the one or more carboxyl groups to form an O-acylisourea intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), to provide an N H S ester-modified GQD; and contacting the N H S ester-modified G Q D with biomolecules of insulin to form the insulin -GQD conjugat,,,,,, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell. Currently amended
The method of claim 1, further comprising contacting the insulin -GQD conjugate with hydroxylamine solution. Currently amended
1 5. The method of claim 1 wherein forming N H S to the one or more carboxyl groups by ester
The method of claim 1 wherein forming the amide bond between a n amine of the biomolecule Currently amended optical imaging is confocal fluorescence subject is a mammal. Withdrawn part of the subject is a mammalian adipocyte the N HS ester-modified GQD comprises coupling bonds. Previously presented insulin -GQD conjugate comprises forming an of insulin and a carboxyl group of GQD.
3-5. Canceled
Canceled
A fluorophore comprising a n insulin-GQD conjugate, wherein a single biomolecule of insulin is coupled to a single GQD, obtained by a method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride reacts with one or more carboxyl groups present on an edge of the GQD to form an O -acylisour e a intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), wherein the O-acylisourea intermediate compound reacts with the N-hydroxysucci ni mide (NH S) to form a N H S ester-modified GQD; and contacting the N H S ester-modified GQD with biomolecules of insulin to form o an am n ea row ac or, su, u,,,, the insulin -GQD conjugate, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell. Currently amended
7-8. Canceled
Canceled
(Withdrawn-Currently Amended) An imaging method, comprising: administering a fluorophore to a subject, wherein the fluorophore comprises an insulin -GQD conjugate, wherein a single biomolecule of insulin is coupled to a single GQD, obtained by a method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the 1-ethyl-3-(3-dimethylami n opropyl) carbodiimide hydrochloride reacts with one or more carboxyl groups present on an edge of the GQD to form an O-acylisourea intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), wherein the O-acylisourea intermediate compound reacts with the N-hydroxysuccini m ide (NH S) to form a N H S ester-modified GQD; and contacting the N H S ester-modified GQD with biomolecules of insulin to form,,,,,, the insulin -GQD conjugate, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell; and collecting imaging data of the subject or part of the subject with an optical imaging. Currently amended
1 0. The imaging method of claim 9, wherein the optical imaging comprises optical multimodality imaging. Withdrawn
The imaging method of claim 9, wherein the optical imaging is ex vivo imaging. Withdrawn
The imaging method of claim 9, wherein the imaging. Withdrawn
The imaging method of claim 9, wherein the
Layer stacks claimed or described, ordered top of device to substrate.
insulin-GQD fluorophore
Materials described outside the worked examples.
graphene quantum dot (GQD)
insulin
Additional fabrication and treatment steps described in the patent.
Patent
Atlas literature
Patent
US 10,114,024Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method to form an insulin-G Q D conjugate, wherein a single biomolecule of insulin is coupled to a single graphene quantum dot (G Q D), the method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the GQD comprises one or more carboxyl groups on an edge of the GQD; and wherein 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride reacts with the one or more carboxyl groups to form an O-acylisourea intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), to provide an N H S ester-modified GQD; and contacting the N H S ester-modified G Q D with biomolecules of insulin to form the insulin -GQD conjugat,,,,,, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell. Currently amended
The method of claim 1, further comprising contacting the insulin -GQD conjugate with hydroxylamine solution. Currently amended
1 5. The method of claim 1 wherein forming N H S to the one or more carboxyl groups by ester
The method of claim 1 wherein forming the amide bond between a n amine of the biomolecule Currently amended optical imaging is confocal fluorescence subject is a mammal. Withdrawn part of the subject is a mammalian adipocyte the N HS ester-modified GQD comprises coupling bonds. Previously presented insulin -GQD conjugate comprises forming an of insulin and a carboxyl group of GQD.
3-5. Canceled
Canceled
A fluorophore comprising a n insulin-GQD conjugate, wherein a single biomolecule of insulin is coupled to a single GQD, obtained by a method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride reacts with one or more carboxyl groups present on an edge of the GQD to form an O -acylisour e a intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), wherein the O-acylisourea intermediate compound reacts with the N-hydroxysucci ni mide (NH S) to form a N H S ester-modified GQD; and contacting the N H S ester-modified GQD with biomolecules of insulin to form o an am n ea row ac or, su, u,,,, the insulin -GQD conjugate, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell. Currently amended
7-8. Canceled
Canceled
(Withdrawn-Currently Amended) An imaging method, comprising: administering a fluorophore to a subject, wherein the fluorophore comprises an insulin -GQD conjugate, wherein a single biomolecule of insulin is coupled to a single GQD, obtained by a method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the 1-ethyl-3-(3-dimethylami n opropyl) carbodiimide hydrochloride reacts with one or more carboxyl groups present on an edge of the GQD to form an O-acylisourea intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), wherein the O-acylisourea intermediate compound reacts with the N-hydroxysuccini m ide (NH S) to form a N H S ester-modified GQD; and contacting the N H S ester-modified GQD with biomolecules of insulin to form,,,,,, the insulin -GQD conjugate, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell; and collecting imaging data of the subject or part of the subject with an optical imaging. Currently amended
1 0. The imaging method of claim 9, wherein the optical imaging comprises optical multimodality imaging. Withdrawn
The imaging method of claim 9, wherein the optical imaging is ex vivo imaging. Withdrawn
The imaging method of claim 9, wherein the imaging. Withdrawn
The imaging method of claim 9, wherein the
Layer stacks claimed or described, ordered top of device to substrate.
insulin-GQD fluorophore
Materials described outside the worked examples.
graphene quantum dot (GQD)
insulin
Additional fabrication and treatment steps described in the patent.
Patent
Atlas literature
Patent
US 10,114,024Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method to form an insulin-G Q D conjugate, wherein a single biomolecule of insulin is coupled to a single graphene quantum dot (G Q D), the method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the GQD comprises one or more carboxyl groups on an edge of the GQD; and wherein 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride reacts with the one or more carboxyl groups to form an O-acylisourea intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), to provide an N H S ester-modified GQD; and contacting the N H S ester-modified G Q D with biomolecules of insulin to form the insulin -GQD conjugat,,,,,, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell. Currently amended
The method of claim 1, further comprising contacting the insulin -GQD conjugate with hydroxylamine solution. Currently amended
1 5. The method of claim 1 wherein forming N H S to the one or more carboxyl groups by ester
The method of claim 1 wherein forming the amide bond between a n amine of the biomolecule Currently amended optical imaging is confocal fluorescence subject is a mammal. Withdrawn part of the subject is a mammalian adipocyte the N HS ester-modified GQD comprises coupling bonds. Previously presented insulin -GQD conjugate comprises forming an of insulin and a carboxyl group of GQD.
3-5. Canceled
Canceled
A fluorophore comprising a n insulin-GQD conjugate, wherein a single biomolecule of insulin is coupled to a single GQD, obtained by a method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride reacts with one or more carboxyl groups present on an edge of the GQD to form an O -acylisour e a intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), wherein the O-acylisourea intermediate compound reacts with the N-hydroxysucci ni mide (NH S) to form a N H S ester-modified GQD; and contacting the N H S ester-modified GQD with biomolecules of insulin to form o an am n ea row ac or, su, u,,,, the insulin -GQD conjugate, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell. Currently amended
7-8. Canceled
Canceled
(Withdrawn-Currently Amended) An imaging method, comprising: administering a fluorophore to a subject, wherein the fluorophore comprises an insulin -GQD conjugate, wherein a single biomolecule of insulin is coupled to a single GQD, obtained by a method comprising: contacting a solution containing GQD with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), wherein the 1-ethyl-3-(3-dimethylami n opropyl) carbodiimide hydrochloride reacts with one or more carboxyl groups present on an edge of the GQD to form an O-acylisourea intermediate compound; contacting the O-acylisourea intermediate compound with N-hydroxysuccinimide (NH S), wherein the O-acylisourea intermediate compound reacts with the N-hydroxysuccini m ide (NH S) to form a N H S ester-modified GQD; and contacting the N H S ester-modified GQD with biomolecules of insulin to form,,,,,, the insulin -GQD conjugate, wherein the insulin -GQD conjugate is suitable for imaging an interior of a living cell; and collecting imaging data of the subject or part of the subject with an optical imaging. Currently amended
1 0. The imaging method of claim 9, wherein the optical imaging comprises optical multimodality imaging. Withdrawn
The imaging method of claim 9, wherein the optical imaging is ex vivo imaging. Withdrawn
The imaging method of claim 9, wherein the imaging. Withdrawn
The imaging method of claim 9, wherein the
Layer stacks claimed or described, ordered top of device to substrate.
insulin-GQD fluorophore
Materials described outside the worked examples.
graphene quantum dot (GQD)
insulin
Additional fabrication and treatment steps described in the patent.
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)
N-hydroxysuccinimide (NHS)
insulin-GQD conjugate
hydroxylamine solution
nerve growth factor (NGF)
neuropeptide Y
bovine serum albumin
immunoglobulin G
concanavalin A
O-acylisourea intermediate compound
NHS ester-modified GQD
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)
N-hydroxysuccinimide (NHS)
insulin-GQD conjugate
hydroxylamine solution
nerve growth factor (NGF)
neuropeptide Y
bovine serum albumin
immunoglobulin G
concanavalin A
O-acylisourea intermediate compound
NHS ester-modified GQD
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)
N-hydroxysuccinimide (NHS)
insulin-GQD conjugate
hydroxylamine solution
nerve growth factor (NGF)
neuropeptide Y
bovine serum albumin
immunoglobulin G
concanavalin A
O-acylisourea intermediate compound
NHS ester-modified GQD
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)
N-hydroxysuccinimide (NHS)
insulin-GQD conjugate
hydroxylamine solution
nerve growth factor (NGF)
neuropeptide Y
bovine serum albumin
immunoglobulin G
concanavalin A
O-acylisourea intermediate compound
NHS ester-modified GQD