Patent
US 10,344,274METHODS FOR SEPARATING NUCLEIC ACIDS WITH GRAPHENE COATED MAGNETIC BEADS
Kevin Hagedorn
US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 10,344,274Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 is a schematic drawing for how single stranded nucleic acids are isolated. [0008]
FIG. 2 is a flow chart of an embodiment of the process disclosed herein and depicted in
FIG. 3 is a schematic drawing for how a single stranded nucleic acid in a sample may be isolated and may also be quantified. [0010]
FIG. 4 is a flow chart for the process of how a single stranded nucleic acid in a sample may be isolated and may also be quantified. [0011]
FIG. 5 is a transmission electron micrographs of a carbon coated magnetic beads which may be used in this process. [0012]
FIG. 6 is a flow chart for the process of separating and producing a single stranded nucleic acid process. [0013]
FIG. 7 is a gel electrophoresis image showing nucleic acids extracted from MCF₇ immortalized breast cancer cells using 125 p l of: 2M, 3M, 4M, 5M, and 6M …
FIG. 8 is a gel electrophoresis image showing nucleic acids extracted from MCF₇ immortalized breast cancer cells using 125 p l of 6M guanidinium thiocyanate …
FIG. 9 is a gel electrophoresis image comparing nucleic acids extracted from MCF₇ immortalized breast cancer cells using a buffer composed of 125 p l of 2M …
FIG. 10 is a flow chart for the process of how sequence specific analysis may be performed using chaotropic salts, alcohols, and carbon coated materials.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method for separating single stranded ribonucleic acid from associated biological material, the method comprising: creating a complex of a three-dimensional carbon coated material and single stranded ribonucleic acid from a mixture containing single stranded ribonucleic acids, double stranded nucleic acids, the carbon coated material, at least one chaotropic salt and at least one alcohol having between one and four carbon atoms, wherein the at least one chaotropic salt present at a concentration from 0. 0 1M to 3M, wherein the alcohols are present as 5 vol% to 99 vol %, and wherein the carbon coating present on the three-dimensional carbon coated material is selected from the group consisting of graphene, pyrolytic carbon and mixtures thereof; and after the complex of the three-dimensional carbon coated material and single stranded ribonucleic acid has been created, removing the three-dimensional carbon coated material complexed with single-stranded ribonucleic acid from the mixture. Currently amended
The method of claim 1, wherein chaotropic salt is a guanidinium salt composed of a guanidinium cation and an anion selected from the group consisting of chloride, bromide, fluoride, acetate, sulfate, nitrate, carbonate, thiocyanate and mixtures thereon. Original
The method of claim 1, wherein the chaotropic salt is present at a concentration between 0.01 M and 0.6M. Original
The method of claim 1, wherein the alcohol is present in an amount between 10 vol% and vol%. Original
The method of claim 1, wherein the carbon-coated carbon coated material comprises a substrate composed of a particulate core having an outer surface and an outer carbon coating overlying the outer surface of the particulate core, the outer carbon coating having a thickness between 1 angstrom and 50 nm. Previously presented
. Canceled
A method for isolating nucleic acids having a defined sequence from an aqueous solution containing single stranded ribonucleic acid and double stranded nucleic acid after the aqueous solution has been contacted with at least one nucleic acid probe that is complementary with amino acids present on at least a portion of nucleic acid present in the aqueous solution, for an interval sufficient for the at least one nucleic acid probe to duplex with amino acid sequences present on at least a portion of the nucleic acid present in the aqueous solution to produce an admixture composed of duplexed nucleic acid material and non- duplexed nucleic acid material, the method comprising: to the admixture containing duplexed nucleic acid material and nonduplexed single-stranded ribonucleic acid material, adding carbon-coated material, at least one chaotropic salt and short-chain alcohol, the short-chain alcohol present in an amount between 2 vol% and 99 vol%, the carbon coated material having a substrate having a core, the core having an outer surface, the carbon coated material further having an outer carbon coating overlying the outer surface of the core, the outer carbon coating having a thickness between 1 angstrom and 50 nm; allowing contact for an interval sufficient to permit association between the carbon coated material and at least a portion of the single stranded ribonucleic acid material present in the admixture; and separating carbon-coated material complexed with the single stranded ribonucleic acid material from liquid supe rn atant, the liquid supe rn atant containing duplexed double stranded nucleic acid. Currently amended
The method of claim 6, wherein the carbon coated material is configured as beads, wherein at least the core is magnetic and contains at least 30% by weight of a metal selected from the group consisting of Ni, Fe, Co, or mixtures thereof and wherein the carbon coating is selected from the group consisting of graphene, pyrolytic carbon and mixtures thereof. Previously presented
The method of claim 6, wherein the chaotropic salt is added in an amount sufficient to provide a concentration between 0. 0 1M and 3M. Original
The method of claim 6, wherein the short chain alcohol is selected from the group consisting of methanol, ethanol, propanol, isopropanol, phenol, and mixtures thereof. Previously presented
The method of claim 6, wherein the separating step results in a complex of carbon-coated magnetic beads and nucleic acid, and the separating step includes subjecting the omplex of carbon-coated magnetic beads and nucleic acid to at least one of a magnetic field, a centrifugal force, precipitation, competitive binding, or mixtures thereof. Currently amended
The method of claim 6, wherein a concentration of the short-chain alcohol is between 10% and 95%. Original
The method of claim 6, further comprising: after separating the carbon-coated material from the liquid supe rn atant, releasing single stranded nucleic acid from attachment to the separated carbon-coated material, wherein the releasing step occurs with addition of water or suitable buffer solution into contact with the separated carbon-coated material. Currently amended
The method of claim 6, wherein the carbon-coated material is configured as individual beads, each bead having an interior core and an outer surface, the beads composed of one of the following: a magnetic metal containing at 30% by weight of a metal selected from the group consisting of Ni, Fe, Co, or mixtures thereof, silica or polymeric substrates, and a carbon coating, wherein the carbon coating is present as a carbon layer on at least a portion of the outer surface of the individual beads, the carbon layer comprising at least one of graphene, pyrolytic carbon or a mixture of graphene and pyrolytic carbon, and wherein multivalent cations are associated with alkali earth metals or alkali earth metal salts or mixtures thereof. Currently amended
The method of claim 6, wherein the at least one nucleic acid probe is a chain of nucleic acids selected such that the chain of nucleic acids contains omplementary bases with corresponding positions to the single stranded nucleic acid that is to be isolated or purified. Currently amended
The method of claim 6, wherein the at least synthetic nucleic acid s and wherein the method sequence for study by performing PCR on the liquid carbon coated magnetic material and associated one nucleic acid probe may be DNA or a further comprises the step of quantifying the supe rn atant that remains after removal of single stranded nucleic acids. Currently amended
Layer stacks claimed or described, ordered top of device to substrate.
carbon coated magnetic bead
Materials described outside the worked examples.
carbon coated material/carbon coated magnetic beads
single stranded ribonucleic acid (ssRNA)
Measurements and analyses referenced in the patent, with their drawing references.
FIG. 5 is a transmission electron micrographs of a carbon coated magnetic beads which may be used in this process. [0012]
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Thickness | 20–100000 nm | — |
Thickness | 0.1–50 nm |
Patent
Atlas literature
Patent
US 10,344,274Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 is a schematic drawing for how single stranded nucleic acids are isolated. [0008]
FIG. 2 is a flow chart of an embodiment of the process disclosed herein and depicted in
FIG. 3 is a schematic drawing for how a single stranded nucleic acid in a sample may be isolated and may also be quantified. [0010]
FIG. 4 is a flow chart for the process of how a single stranded nucleic acid in a sample may be isolated and may also be quantified. [0011]
FIG. 5 is a transmission electron micrographs of a carbon coated magnetic beads which may be used in this process. [0012]
FIG. 6 is a flow chart for the process of separating and producing a single stranded nucleic acid process. [0013]
FIG. 7 is a gel electrophoresis image showing nucleic acids extracted from MCF₇ immortalized breast cancer cells using 125 p l of: 2M, 3M, 4M, 5M, and 6M …
FIG. 8 is a gel electrophoresis image showing nucleic acids extracted from MCF₇ immortalized breast cancer cells using 125 p l of 6M guanidinium thiocyanate …
FIG. 9 is a gel electrophoresis image comparing nucleic acids extracted from MCF₇ immortalized breast cancer cells using a buffer composed of 125 p l of 2M …
FIG. 10 is a flow chart for the process of how sequence specific analysis may be performed using chaotropic salts, alcohols, and carbon coated materials.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method for separating single stranded ribonucleic acid from associated biological material, the method comprising: creating a complex of a three-dimensional carbon coated material and single stranded ribonucleic acid from a mixture containing single stranded ribonucleic acids, double stranded nucleic acids, the carbon coated material, at least one chaotropic salt and at least one alcohol having between one and four carbon atoms, wherein the at least one chaotropic salt present at a concentration from 0. 0 1M to 3M, wherein the alcohols are present as 5 vol% to 99 vol %, and wherein the carbon coating present on the three-dimensional carbon coated material is selected from the group consisting of graphene, pyrolytic carbon and mixtures thereof; and after the complex of the three-dimensional carbon coated material and single stranded ribonucleic acid has been created, removing the three-dimensional carbon coated material complexed with single-stranded ribonucleic acid from the mixture. Currently amended
The method of claim 1, wherein chaotropic salt is a guanidinium salt composed of a guanidinium cation and an anion selected from the group consisting of chloride, bromide, fluoride, acetate, sulfate, nitrate, carbonate, thiocyanate and mixtures thereon. Original
The method of claim 1, wherein the chaotropic salt is present at a concentration between 0.01 M and 0.6M. Original
The method of claim 1, wherein the alcohol is present in an amount between 10 vol% and vol%. Original
The method of claim 1, wherein the carbon-coated carbon coated material comprises a substrate composed of a particulate core having an outer surface and an outer carbon coating overlying the outer surface of the particulate core, the outer carbon coating having a thickness between 1 angstrom and 50 nm. Previously presented
. Canceled
A method for isolating nucleic acids having a defined sequence from an aqueous solution containing single stranded ribonucleic acid and double stranded nucleic acid after the aqueous solution has been contacted with at least one nucleic acid probe that is complementary with amino acids present on at least a portion of nucleic acid present in the aqueous solution, for an interval sufficient for the at least one nucleic acid probe to duplex with amino acid sequences present on at least a portion of the nucleic acid present in the aqueous solution to produce an admixture composed of duplexed nucleic acid material and non- duplexed nucleic acid material, the method comprising: to the admixture containing duplexed nucleic acid material and nonduplexed single-stranded ribonucleic acid material, adding carbon-coated material, at least one chaotropic salt and short-chain alcohol, the short-chain alcohol present in an amount between 2 vol% and 99 vol%, the carbon coated material having a substrate having a core, the core having an outer surface, the carbon coated material further having an outer carbon coating overlying the outer surface of the core, the outer carbon coating having a thickness between 1 angstrom and 50 nm; allowing contact for an interval sufficient to permit association between the carbon coated material and at least a portion of the single stranded ribonucleic acid material present in the admixture; and separating carbon-coated material complexed with the single stranded ribonucleic acid material from liquid supe rn atant, the liquid supe rn atant containing duplexed double stranded nucleic acid. Currently amended
The method of claim 6, wherein the carbon coated material is configured as beads, wherein at least the core is magnetic and contains at least 30% by weight of a metal selected from the group consisting of Ni, Fe, Co, or mixtures thereof and wherein the carbon coating is selected from the group consisting of graphene, pyrolytic carbon and mixtures thereof. Previously presented
The method of claim 6, wherein the chaotropic salt is added in an amount sufficient to provide a concentration between 0. 0 1M and 3M. Original
The method of claim 6, wherein the short chain alcohol is selected from the group consisting of methanol, ethanol, propanol, isopropanol, phenol, and mixtures thereof. Previously presented
The method of claim 6, wherein the separating step results in a complex of carbon-coated magnetic beads and nucleic acid, and the separating step includes subjecting the omplex of carbon-coated magnetic beads and nucleic acid to at least one of a magnetic field, a centrifugal force, precipitation, competitive binding, or mixtures thereof. Currently amended
The method of claim 6, wherein a concentration of the short-chain alcohol is between 10% and 95%. Original
The method of claim 6, further comprising: after separating the carbon-coated material from the liquid supe rn atant, releasing single stranded nucleic acid from attachment to the separated carbon-coated material, wherein the releasing step occurs with addition of water or suitable buffer solution into contact with the separated carbon-coated material. Currently amended
The method of claim 6, wherein the carbon-coated material is configured as individual beads, each bead having an interior core and an outer surface, the beads composed of one of the following: a magnetic metal containing at 30% by weight of a metal selected from the group consisting of Ni, Fe, Co, or mixtures thereof, silica or polymeric substrates, and a carbon coating, wherein the carbon coating is present as a carbon layer on at least a portion of the outer surface of the individual beads, the carbon layer comprising at least one of graphene, pyrolytic carbon or a mixture of graphene and pyrolytic carbon, and wherein multivalent cations are associated with alkali earth metals or alkali earth metal salts or mixtures thereof. Currently amended
The method of claim 6, wherein the at least one nucleic acid probe is a chain of nucleic acids selected such that the chain of nucleic acids contains omplementary bases with corresponding positions to the single stranded nucleic acid that is to be isolated or purified. Currently amended
The method of claim 6, wherein the at least synthetic nucleic acid s and wherein the method sequence for study by performing PCR on the liquid carbon coated magnetic material and associated one nucleic acid probe may be DNA or a further comprises the step of quantifying the supe rn atant that remains after removal of single stranded nucleic acids. Currently amended
Layer stacks claimed or described, ordered top of device to substrate.
carbon coated magnetic bead
Materials described outside the worked examples.
carbon coated material/carbon coated magnetic beads
single stranded ribonucleic acid (ssRNA)
Measurements and analyses referenced in the patent, with their drawing references.
FIG. 5 is a transmission electron micrographs of a carbon coated magnetic beads which may be used in this process. [0012]
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Thickness | 20–100000 nm | — |
Thickness | 0.1–50 nm |
Patent
Atlas literature
Patent
US 10,344,274Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 is a schematic drawing for how single stranded nucleic acids are isolated. [0008]
FIG. 2 is a flow chart of an embodiment of the process disclosed herein and depicted in
FIG. 3 is a schematic drawing for how a single stranded nucleic acid in a sample may be isolated and may also be quantified. [0010]
FIG. 4 is a flow chart for the process of how a single stranded nucleic acid in a sample may be isolated and may also be quantified. [0011]
FIG. 5 is a transmission electron micrographs of a carbon coated magnetic beads which may be used in this process. [0012]
FIG. 6 is a flow chart for the process of separating and producing a single stranded nucleic acid process. [0013]
FIG. 7 is a gel electrophoresis image showing nucleic acids extracted from MCF₇ immortalized breast cancer cells using 125 p l of: 2M, 3M, 4M, 5M, and 6M …
FIG. 8 is a gel electrophoresis image showing nucleic acids extracted from MCF₇ immortalized breast cancer cells using 125 p l of 6M guanidinium thiocyanate …
FIG. 9 is a gel electrophoresis image comparing nucleic acids extracted from MCF₇ immortalized breast cancer cells using a buffer composed of 125 p l of 2M …
FIG. 10 is a flow chart for the process of how sequence specific analysis may be performed using chaotropic salts, alcohols, and carbon coated materials.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method for separating single stranded ribonucleic acid from associated biological material, the method comprising: creating a complex of a three-dimensional carbon coated material and single stranded ribonucleic acid from a mixture containing single stranded ribonucleic acids, double stranded nucleic acids, the carbon coated material, at least one chaotropic salt and at least one alcohol having between one and four carbon atoms, wherein the at least one chaotropic salt present at a concentration from 0. 0 1M to 3M, wherein the alcohols are present as 5 vol% to 99 vol %, and wherein the carbon coating present on the three-dimensional carbon coated material is selected from the group consisting of graphene, pyrolytic carbon and mixtures thereof; and after the complex of the three-dimensional carbon coated material and single stranded ribonucleic acid has been created, removing the three-dimensional carbon coated material complexed with single-stranded ribonucleic acid from the mixture. Currently amended
The method of claim 1, wherein chaotropic salt is a guanidinium salt composed of a guanidinium cation and an anion selected from the group consisting of chloride, bromide, fluoride, acetate, sulfate, nitrate, carbonate, thiocyanate and mixtures thereon. Original
The method of claim 1, wherein the chaotropic salt is present at a concentration between 0.01 M and 0.6M. Original
The method of claim 1, wherein the alcohol is present in an amount between 10 vol% and vol%. Original
The method of claim 1, wherein the carbon-coated carbon coated material comprises a substrate composed of a particulate core having an outer surface and an outer carbon coating overlying the outer surface of the particulate core, the outer carbon coating having a thickness between 1 angstrom and 50 nm. Previously presented
. Canceled
A method for isolating nucleic acids having a defined sequence from an aqueous solution containing single stranded ribonucleic acid and double stranded nucleic acid after the aqueous solution has been contacted with at least one nucleic acid probe that is complementary with amino acids present on at least a portion of nucleic acid present in the aqueous solution, for an interval sufficient for the at least one nucleic acid probe to duplex with amino acid sequences present on at least a portion of the nucleic acid present in the aqueous solution to produce an admixture composed of duplexed nucleic acid material and non- duplexed nucleic acid material, the method comprising: to the admixture containing duplexed nucleic acid material and nonduplexed single-stranded ribonucleic acid material, adding carbon-coated material, at least one chaotropic salt and short-chain alcohol, the short-chain alcohol present in an amount between 2 vol% and 99 vol%, the carbon coated material having a substrate having a core, the core having an outer surface, the carbon coated material further having an outer carbon coating overlying the outer surface of the core, the outer carbon coating having a thickness between 1 angstrom and 50 nm; allowing contact for an interval sufficient to permit association between the carbon coated material and at least a portion of the single stranded ribonucleic acid material present in the admixture; and separating carbon-coated material complexed with the single stranded ribonucleic acid material from liquid supe rn atant, the liquid supe rn atant containing duplexed double stranded nucleic acid. Currently amended
The method of claim 6, wherein the carbon coated material is configured as beads, wherein at least the core is magnetic and contains at least 30% by weight of a metal selected from the group consisting of Ni, Fe, Co, or mixtures thereof and wherein the carbon coating is selected from the group consisting of graphene, pyrolytic carbon and mixtures thereof. Previously presented
The method of claim 6, wherein the chaotropic salt is added in an amount sufficient to provide a concentration between 0. 0 1M and 3M. Original
The method of claim 6, wherein the short chain alcohol is selected from the group consisting of methanol, ethanol, propanol, isopropanol, phenol, and mixtures thereof. Previously presented
The method of claim 6, wherein the separating step results in a complex of carbon-coated magnetic beads and nucleic acid, and the separating step includes subjecting the omplex of carbon-coated magnetic beads and nucleic acid to at least one of a magnetic field, a centrifugal force, precipitation, competitive binding, or mixtures thereof. Currently amended
The method of claim 6, wherein a concentration of the short-chain alcohol is between 10% and 95%. Original
The method of claim 6, further comprising: after separating the carbon-coated material from the liquid supe rn atant, releasing single stranded nucleic acid from attachment to the separated carbon-coated material, wherein the releasing step occurs with addition of water or suitable buffer solution into contact with the separated carbon-coated material. Currently amended
The method of claim 6, wherein the carbon-coated material is configured as individual beads, each bead having an interior core and an outer surface, the beads composed of one of the following: a magnetic metal containing at 30% by weight of a metal selected from the group consisting of Ni, Fe, Co, or mixtures thereof, silica or polymeric substrates, and a carbon coating, wherein the carbon coating is present as a carbon layer on at least a portion of the outer surface of the individual beads, the carbon layer comprising at least one of graphene, pyrolytic carbon or a mixture of graphene and pyrolytic carbon, and wherein multivalent cations are associated with alkali earth metals or alkali earth metal salts or mixtures thereof. Currently amended
The method of claim 6, wherein the at least one nucleic acid probe is a chain of nucleic acids selected such that the chain of nucleic acids contains omplementary bases with corresponding positions to the single stranded nucleic acid that is to be isolated or purified. Currently amended
The method of claim 6, wherein the at least synthetic nucleic acid s and wherein the method sequence for study by performing PCR on the liquid carbon coated magnetic material and associated one nucleic acid probe may be DNA or a further comprises the step of quantifying the supe rn atant that remains after removal of single stranded nucleic acids. Currently amended
Layer stacks claimed or described, ordered top of device to substrate.
carbon coated magnetic bead
Materials described outside the worked examples.
carbon coated material/carbon coated magnetic beads
single stranded ribonucleic acid (ssRNA)
Measurements and analyses referenced in the patent, with their drawing references.
FIG. 5 is a transmission electron micrographs of a carbon coated magnetic beads which may be used in this process. [0012]
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Thickness | 20–100000 nm | — |
Thickness | 0.1–50 nm |
Patent
Atlas literature
Patent
US 10,344,274Patent drawings and their descriptions. Click a drawing to enlarge it.
FIG. 1 is a schematic drawing for how single stranded nucleic acids are isolated. [0008]
FIG. 2 is a flow chart of an embodiment of the process disclosed herein and depicted in
FIG. 3 is a schematic drawing for how a single stranded nucleic acid in a sample may be isolated and may also be quantified. [0010]
FIG. 4 is a flow chart for the process of how a single stranded nucleic acid in a sample may be isolated and may also be quantified. [0011]
FIG. 5 is a transmission electron micrographs of a carbon coated magnetic beads which may be used in this process. [0012]
FIG. 6 is a flow chart for the process of separating and producing a single stranded nucleic acid process. [0013]
FIG. 7 is a gel electrophoresis image showing nucleic acids extracted from MCF₇ immortalized breast cancer cells using 125 p l of: 2M, 3M, 4M, 5M, and 6M …
FIG. 8 is a gel electrophoresis image showing nucleic acids extracted from MCF₇ immortalized breast cancer cells using 125 p l of 6M guanidinium thiocyanate …
FIG. 9 is a gel electrophoresis image comparing nucleic acids extracted from MCF₇ immortalized breast cancer cells using a buffer composed of 125 p l of 2M …
FIG. 10 is a flow chart for the process of how sequence specific analysis may be performed using chaotropic salts, alcohols, and carbon coated materials.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method for separating single stranded ribonucleic acid from associated biological material, the method comprising: creating a complex of a three-dimensional carbon coated material and single stranded ribonucleic acid from a mixture containing single stranded ribonucleic acids, double stranded nucleic acids, the carbon coated material, at least one chaotropic salt and at least one alcohol having between one and four carbon atoms, wherein the at least one chaotropic salt present at a concentration from 0. 0 1M to 3M, wherein the alcohols are present as 5 vol% to 99 vol %, and wherein the carbon coating present on the three-dimensional carbon coated material is selected from the group consisting of graphene, pyrolytic carbon and mixtures thereof; and after the complex of the three-dimensional carbon coated material and single stranded ribonucleic acid has been created, removing the three-dimensional carbon coated material complexed with single-stranded ribonucleic acid from the mixture. Currently amended
The method of claim 1, wherein chaotropic salt is a guanidinium salt composed of a guanidinium cation and an anion selected from the group consisting of chloride, bromide, fluoride, acetate, sulfate, nitrate, carbonate, thiocyanate and mixtures thereon. Original
The method of claim 1, wherein the chaotropic salt is present at a concentration between 0.01 M and 0.6M. Original
The method of claim 1, wherein the alcohol is present in an amount between 10 vol% and vol%. Original
The method of claim 1, wherein the carbon-coated carbon coated material comprises a substrate composed of a particulate core having an outer surface and an outer carbon coating overlying the outer surface of the particulate core, the outer carbon coating having a thickness between 1 angstrom and 50 nm. Previously presented
. Canceled
A method for isolating nucleic acids having a defined sequence from an aqueous solution containing single stranded ribonucleic acid and double stranded nucleic acid after the aqueous solution has been contacted with at least one nucleic acid probe that is complementary with amino acids present on at least a portion of nucleic acid present in the aqueous solution, for an interval sufficient for the at least one nucleic acid probe to duplex with amino acid sequences present on at least a portion of the nucleic acid present in the aqueous solution to produce an admixture composed of duplexed nucleic acid material and non- duplexed nucleic acid material, the method comprising: to the admixture containing duplexed nucleic acid material and nonduplexed single-stranded ribonucleic acid material, adding carbon-coated material, at least one chaotropic salt and short-chain alcohol, the short-chain alcohol present in an amount between 2 vol% and 99 vol%, the carbon coated material having a substrate having a core, the core having an outer surface, the carbon coated material further having an outer carbon coating overlying the outer surface of the core, the outer carbon coating having a thickness between 1 angstrom and 50 nm; allowing contact for an interval sufficient to permit association between the carbon coated material and at least a portion of the single stranded ribonucleic acid material present in the admixture; and separating carbon-coated material complexed with the single stranded ribonucleic acid material from liquid supe rn atant, the liquid supe rn atant containing duplexed double stranded nucleic acid. Currently amended
The method of claim 6, wherein the carbon coated material is configured as beads, wherein at least the core is magnetic and contains at least 30% by weight of a metal selected from the group consisting of Ni, Fe, Co, or mixtures thereof and wherein the carbon coating is selected from the group consisting of graphene, pyrolytic carbon and mixtures thereof. Previously presented
The method of claim 6, wherein the chaotropic salt is added in an amount sufficient to provide a concentration between 0. 0 1M and 3M. Original
The method of claim 6, wherein the short chain alcohol is selected from the group consisting of methanol, ethanol, propanol, isopropanol, phenol, and mixtures thereof. Previously presented
The method of claim 6, wherein the separating step results in a complex of carbon-coated magnetic beads and nucleic acid, and the separating step includes subjecting the omplex of carbon-coated magnetic beads and nucleic acid to at least one of a magnetic field, a centrifugal force, precipitation, competitive binding, or mixtures thereof. Currently amended
The method of claim 6, wherein a concentration of the short-chain alcohol is between 10% and 95%. Original
The method of claim 6, further comprising: after separating the carbon-coated material from the liquid supe rn atant, releasing single stranded nucleic acid from attachment to the separated carbon-coated material, wherein the releasing step occurs with addition of water or suitable buffer solution into contact with the separated carbon-coated material. Currently amended
The method of claim 6, wherein the carbon-coated material is configured as individual beads, each bead having an interior core and an outer surface, the beads composed of one of the following: a magnetic metal containing at 30% by weight of a metal selected from the group consisting of Ni, Fe, Co, or mixtures thereof, silica or polymeric substrates, and a carbon coating, wherein the carbon coating is present as a carbon layer on at least a portion of the outer surface of the individual beads, the carbon layer comprising at least one of graphene, pyrolytic carbon or a mixture of graphene and pyrolytic carbon, and wherein multivalent cations are associated with alkali earth metals or alkali earth metal salts or mixtures thereof. Currently amended
The method of claim 6, wherein the at least one nucleic acid probe is a chain of nucleic acids selected such that the chain of nucleic acids contains omplementary bases with corresponding positions to the single stranded nucleic acid that is to be isolated or purified. Currently amended
The method of claim 6, wherein the at least synthetic nucleic acid s and wherein the method sequence for study by performing PCR on the liquid carbon coated magnetic material and associated one nucleic acid probe may be DNA or a further comprises the step of quantifying the supe rn atant that remains after removal of single stranded nucleic acids. Currently amended
Layer stacks claimed or described, ordered top of device to substrate.
carbon coated magnetic bead
Materials described outside the worked examples.
carbon coated material/carbon coated magnetic beads
single stranded ribonucleic acid (ssRNA)
Measurements and analyses referenced in the patent, with their drawing references.
FIG. 5 is a transmission electron micrographs of a carbon coated magnetic beads which may be used in this process. [0012]
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
Thickness | 20–100000 nm | — |
Thickness | 0.1–50 nm |
chaotropic salt
short-chain alcohol (1-4 carbons)
graphene
pyrolytic carbon
magnetic metal core (Ni, Fe, Co or mixtures)
guanidinium thiocyanate
ethanol
C₂H₅OH
EDTA
| — |
Duration | 1–300 s | — |
Duration | 10–300 s | — |
Duration | 10–86400 s | — |
Thickness | 20–200 nm | — |
Thickness | 10–100000 nm | — |
Thickness | 200–1500 nm | — |
Thickness | 50–500 mM | — |
Thickness | 1–100 mM | — |
Thickness | 0.1–5 mM | — |
chaotropic salt
short-chain alcohol (1-4 carbons)
graphene
pyrolytic carbon
magnetic metal core (Ni, Fe, Co or mixtures)
guanidinium thiocyanate
ethanol
C₂H₅OH
EDTA
| — |
Duration | 1–300 s | — |
Duration | 10–300 s | — |
Duration | 10–86400 s | — |
Thickness | 20–200 nm | — |
Thickness | 10–100000 nm | — |
Thickness | 200–1500 nm | — |
Thickness | 50–500 mM | — |
Thickness | 1–100 mM | — |
Thickness | 0.1–5 mM | — |
chaotropic salt
short-chain alcohol (1-4 carbons)
graphene
pyrolytic carbon
magnetic metal core (Ni, Fe, Co or mixtures)
guanidinium thiocyanate
ethanol
C₂H₅OH
EDTA
| — |
Duration | 1–300 s | — |
Duration | 10–300 s | — |
Duration | 10–86400 s | — |
Thickness | 20–200 nm | — |
Thickness | 10–100000 nm | — |
Thickness | 200–1500 nm | — |
Thickness | 50–500 mM | — |
Thickness | 1–100 mM | — |
Thickness | 0.1–5 mM | — |
chaotropic salt
short-chain alcohol (1-4 carbons)
graphene
pyrolytic carbon
magnetic metal core (Ni, Fe, Co or mixtures)
guanidinium thiocyanate
ethanol
C₂H₅OH
EDTA
| — |
Duration | 1–300 s | — |
Duration | 10–300 s | — |
Duration | 10–86400 s | — |
Thickness | 20–200 nm | — |
Thickness | 10–100000 nm | — |
Thickness | 200–1500 nm | — |
Thickness | 50–500 mM | — |
Thickness | 1–100 mM | — |
Thickness | 0.1–5 mM | — |