Patent
US 9,091,640CHITOSAN-GRAPHENE BIOSENSORS AND METHODS FOR THEIR USE
Shyam S. Mohapatra
US
Copyright © 2026 Matter42. All rights reserved.
Patent
Atlas literature
Patent
US 9,091,640Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates nonhybridization of the capture and target polynucleotides.
The method of claim 1, wherein non-hybridization indicates a mutation in the target polynucleotide as compared to the hybridizing control polynucleotide.
The method of claim 1, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution.
The method of claim 1, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution.
The method of claim 1, wherein chitosan-graphene ratio is approximately 1:1.
The method of claim 1, wherein K 4 [Fe(CN) 6] 3 -1 4-is a redox probe in the cyclic voltammetry.
A method for detecting a mutation in a single stranded target polynucleotide sequence, comprising a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material and having a single stranded capture polynucleotide attached thereto; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation in the target polynucleotide as compared to the control.
The method of claim 7, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution.
The method of claim 7, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution.
The method of claim 7, wherein chitosan: graphene ratio is approximately 1:1.
The method of claim 7, wherein K 4 [Fe(CN) 6] 3 -1 4-is a redox probe in the cyclic voltammetry.
A device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence as compared to a control, comprising a glassy carbon electrode (GCE) coated with a chitosan-graphene nanosheet material, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene and 0.005% (w/v) chitosan solution, and wherein the capture polynucleotide is attached to the chitosan- graphene nanosheet material.
The device of claim 12, wherein an FT-IR spectrum analysis of the chitosan-graphene nanosheet material exhibits IR peaks at approximately 2920 cm⁻¹ and 2849 cm⁻¹ (sp 2 vC- H stretching), approximately 1731 cm⁻¹ and 1626 cm⁻¹ (v c=o), -4- approximately 1481 cm⁻¹ (skeletal vibrations of graphene sheets), approximately 1461 cm- 1 (carboxyl v c-o stretching), approximately 1177 cm⁻¹ (alkoxy v c-o stretching at the edges of graphene), approximately 1051 cm 1 (vc-o stretching), and approximately 1635 cm- 1 (vCO NH).
A method for making a device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence as compared to a control, comprising providing a glassy carbon electrode (GCE) and coating the G CE with a chitosan-graphene nanosheet material, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene and 0.005% (w/v) chitosan solution, and wherein the capture polynucleotide is attached to the chitosan-graphene nanosheet material.
The method of claim 14, wherein an FT-IR spectrum analysis of the chitosan-graphene nanosheet material exhibits IR peaks at approximately 2920 cm⁻¹ and 2849 cm⁻¹ (sp 2 VC- H stretching), approximately 1731 cm⁻¹ and 1626 cm⁻¹ (vc =o), approximately 1481 cm⁻¹ (skeletal vibrations of graphene sheets), approximately 1461 cm- 1 (carboxyl v c-o stretching), approximately 1177 cm⁻¹ (alkoxy v c-o stretching at the edges of graphene), approximately 1051 cm 1 (v c-o stretching), and approximately 1635 cm- 1 (vCO NH).
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non- hybridization of the capture and target polynucleotides.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; -6- c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non-hybridization of the capture and target polynucleotides.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein chitosan-graphene ratio is approximately 1:1; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non-hybridization of the capture and target polynucleotides.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached -7- thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein chitosan-graphene ratio is approximately 1:1; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control. -9-
Layer stacks claimed or described, ordered top of device to substrate.
chitosan-graphene modified glassy carbon electrode (CMG electrode)
Materials described outside the worked examples.
chitosan-graphene nanosheet material
chitosan
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
sp2 vC-H stretching | 2920 cm⁻¹ | chitosan-graphene nanosheet material |
Patent
Atlas literature
Patent
US 9,091,640Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates nonhybridization of the capture and target polynucleotides.
The method of claim 1, wherein non-hybridization indicates a mutation in the target polynucleotide as compared to the hybridizing control polynucleotide.
The method of claim 1, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution.
The method of claim 1, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution.
The method of claim 1, wherein chitosan-graphene ratio is approximately 1:1.
The method of claim 1, wherein K 4 [Fe(CN) 6] 3 -1 4-is a redox probe in the cyclic voltammetry.
A method for detecting a mutation in a single stranded target polynucleotide sequence, comprising a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material and having a single stranded capture polynucleotide attached thereto; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation in the target polynucleotide as compared to the control.
The method of claim 7, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution.
The method of claim 7, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution.
The method of claim 7, wherein chitosan: graphene ratio is approximately 1:1.
The method of claim 7, wherein K 4 [Fe(CN) 6] 3 -1 4-is a redox probe in the cyclic voltammetry.
A device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence as compared to a control, comprising a glassy carbon electrode (GCE) coated with a chitosan-graphene nanosheet material, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene and 0.005% (w/v) chitosan solution, and wherein the capture polynucleotide is attached to the chitosan- graphene nanosheet material.
The device of claim 12, wherein an FT-IR spectrum analysis of the chitosan-graphene nanosheet material exhibits IR peaks at approximately 2920 cm⁻¹ and 2849 cm⁻¹ (sp 2 vC- H stretching), approximately 1731 cm⁻¹ and 1626 cm⁻¹ (v c=o), -4- approximately 1481 cm⁻¹ (skeletal vibrations of graphene sheets), approximately 1461 cm- 1 (carboxyl v c-o stretching), approximately 1177 cm⁻¹ (alkoxy v c-o stretching at the edges of graphene), approximately 1051 cm 1 (vc-o stretching), and approximately 1635 cm- 1 (vCO NH).
A method for making a device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence as compared to a control, comprising providing a glassy carbon electrode (GCE) and coating the G CE with a chitosan-graphene nanosheet material, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene and 0.005% (w/v) chitosan solution, and wherein the capture polynucleotide is attached to the chitosan-graphene nanosheet material.
The method of claim 14, wherein an FT-IR spectrum analysis of the chitosan-graphene nanosheet material exhibits IR peaks at approximately 2920 cm⁻¹ and 2849 cm⁻¹ (sp 2 VC- H stretching), approximately 1731 cm⁻¹ and 1626 cm⁻¹ (vc =o), approximately 1481 cm⁻¹ (skeletal vibrations of graphene sheets), approximately 1461 cm- 1 (carboxyl v c-o stretching), approximately 1177 cm⁻¹ (alkoxy v c-o stretching at the edges of graphene), approximately 1051 cm 1 (v c-o stretching), and approximately 1635 cm- 1 (vCO NH).
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non- hybridization of the capture and target polynucleotides.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; -6- c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non-hybridization of the capture and target polynucleotides.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein chitosan-graphene ratio is approximately 1:1; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non-hybridization of the capture and target polynucleotides.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached -7- thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein chitosan-graphene ratio is approximately 1:1; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control. -9-
Layer stacks claimed or described, ordered top of device to substrate.
chitosan-graphene modified glassy carbon electrode (CMG electrode)
Materials described outside the worked examples.
chitosan-graphene nanosheet material
chitosan
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
sp2 vC-H stretching | 2920 cm⁻¹ | chitosan-graphene nanosheet material |
Patent
Atlas literature
Patent
US 9,091,640Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates nonhybridization of the capture and target polynucleotides.
The method of claim 1, wherein non-hybridization indicates a mutation in the target polynucleotide as compared to the hybridizing control polynucleotide.
The method of claim 1, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution.
The method of claim 1, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution.
The method of claim 1, wherein chitosan-graphene ratio is approximately 1:1.
The method of claim 1, wherein K 4 [Fe(CN) 6] 3 -1 4-is a redox probe in the cyclic voltammetry.
A method for detecting a mutation in a single stranded target polynucleotide sequence, comprising a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material and having a single stranded capture polynucleotide attached thereto; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation in the target polynucleotide as compared to the control.
The method of claim 7, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution.
The method of claim 7, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution.
The method of claim 7, wherein chitosan: graphene ratio is approximately 1:1.
The method of claim 7, wherein K 4 [Fe(CN) 6] 3 -1 4-is a redox probe in the cyclic voltammetry.
A device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence as compared to a control, comprising a glassy carbon electrode (GCE) coated with a chitosan-graphene nanosheet material, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene and 0.005% (w/v) chitosan solution, and wherein the capture polynucleotide is attached to the chitosan- graphene nanosheet material.
The device of claim 12, wherein an FT-IR spectrum analysis of the chitosan-graphene nanosheet material exhibits IR peaks at approximately 2920 cm⁻¹ and 2849 cm⁻¹ (sp 2 vC- H stretching), approximately 1731 cm⁻¹ and 1626 cm⁻¹ (v c=o), -4- approximately 1481 cm⁻¹ (skeletal vibrations of graphene sheets), approximately 1461 cm- 1 (carboxyl v c-o stretching), approximately 1177 cm⁻¹ (alkoxy v c-o stretching at the edges of graphene), approximately 1051 cm 1 (vc-o stretching), and approximately 1635 cm- 1 (vCO NH).
A method for making a device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence as compared to a control, comprising providing a glassy carbon electrode (GCE) and coating the G CE with a chitosan-graphene nanosheet material, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene and 0.005% (w/v) chitosan solution, and wherein the capture polynucleotide is attached to the chitosan-graphene nanosheet material.
The method of claim 14, wherein an FT-IR spectrum analysis of the chitosan-graphene nanosheet material exhibits IR peaks at approximately 2920 cm⁻¹ and 2849 cm⁻¹ (sp 2 VC- H stretching), approximately 1731 cm⁻¹ and 1626 cm⁻¹ (vc =o), approximately 1481 cm⁻¹ (skeletal vibrations of graphene sheets), approximately 1461 cm- 1 (carboxyl v c-o stretching), approximately 1177 cm⁻¹ (alkoxy v c-o stretching at the edges of graphene), approximately 1051 cm 1 (v c-o stretching), and approximately 1635 cm- 1 (vCO NH).
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non- hybridization of the capture and target polynucleotides.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; -6- c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non-hybridization of the capture and target polynucleotides.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein chitosan-graphene ratio is approximately 1:1; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non-hybridization of the capture and target polynucleotides.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached -7- thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein chitosan-graphene ratio is approximately 1:1; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control. -9-
Layer stacks claimed or described, ordered top of device to substrate.
chitosan-graphene modified glassy carbon electrode (CMG electrode)
Materials described outside the worked examples.
chitosan-graphene nanosheet material
chitosan
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
sp2 vC-H stretching | 2920 cm⁻¹ | chitosan-graphene nanosheet material |
Patent
Atlas literature
Patent
US 9,091,640Patent drawings and their descriptions. Click a drawing to enlarge it.
Claims define the patent's legal scope. Independent claims stand alone; dependent claims (nested) narrow them. Click a claim to expand its dependents.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates nonhybridization of the capture and target polynucleotides.
The method of claim 1, wherein non-hybridization indicates a mutation in the target polynucleotide as compared to the hybridizing control polynucleotide.
The method of claim 1, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution.
The method of claim 1, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution.
The method of claim 1, wherein chitosan-graphene ratio is approximately 1:1.
The method of claim 1, wherein K 4 [Fe(CN) 6] 3 -1 4-is a redox probe in the cyclic voltammetry.
A method for detecting a mutation in a single stranded target polynucleotide sequence, comprising a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material and having a single stranded capture polynucleotide attached thereto; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation in the target polynucleotide as compared to the control.
The method of claim 7, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution.
The method of claim 7, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution.
The method of claim 7, wherein chitosan: graphene ratio is approximately 1:1.
The method of claim 7, wherein K 4 [Fe(CN) 6] 3 -1 4-is a redox probe in the cyclic voltammetry.
A device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence as compared to a control, comprising a glassy carbon electrode (GCE) coated with a chitosan-graphene nanosheet material, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene and 0.005% (w/v) chitosan solution, and wherein the capture polynucleotide is attached to the chitosan- graphene nanosheet material.
The device of claim 12, wherein an FT-IR spectrum analysis of the chitosan-graphene nanosheet material exhibits IR peaks at approximately 2920 cm⁻¹ and 2849 cm⁻¹ (sp 2 vC- H stretching), approximately 1731 cm⁻¹ and 1626 cm⁻¹ (v c=o), -4- approximately 1481 cm⁻¹ (skeletal vibrations of graphene sheets), approximately 1461 cm- 1 (carboxyl v c-o stretching), approximately 1177 cm⁻¹ (alkoxy v c-o stretching at the edges of graphene), approximately 1051 cm 1 (vc-o stretching), and approximately 1635 cm- 1 (vCO NH).
A method for making a device for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence as compared to a control, comprising providing a glassy carbon electrode (GCE) and coating the G CE with a chitosan-graphene nanosheet material, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene and 0.005% (w/v) chitosan solution, and wherein the capture polynucleotide is attached to the chitosan-graphene nanosheet material.
The method of claim 14, wherein an FT-IR spectrum analysis of the chitosan-graphene nanosheet material exhibits IR peaks at approximately 2920 cm⁻¹ and 2849 cm⁻¹ (sp 2 VC- H stretching), approximately 1731 cm⁻¹ and 1626 cm⁻¹ (vc =o), approximately 1481 cm⁻¹ (skeletal vibrations of graphene sheets), approximately 1461 cm- 1 (carboxyl v c-o stretching), approximately 1177 cm⁻¹ (alkoxy v c-o stretching at the edges of graphene), approximately 1051 cm 1 (v c-o stretching), and approximately 1635 cm- 1 (vCO NH).
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non- hybridization of the capture and target polynucleotides.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; -6- c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non-hybridization of the capture and target polynucleotides.
A method for detecting non-hybridization of a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein chitosan-graphene ratio is approximately 1:1; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry; wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates non-hybridization of the capture and target polynucleotides.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached -7- thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) graphene solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein the chitosan-graphene nanosheet material is prepared using an approximately 0.005% (w/v) chitosan solution; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control.
A method for detecting a mutation in a single stranded target polynucleotide sequence and a single stranded capture polynucleotide sequence, comprising: a. providing a glassy carbon electrode (GCE) coated with a chitosan- graphene nanosheet material having the capture polynucleotide attached thereto, wherein chitosan-graphene ratio is approximately 1:1; b. adding the target polynucleotide to the GCE and allowing sufficient time for binding of the capture and target polynucleotides; c. removing the unbound target polynucleotide; and d. subjecting the GCE to cyclic voltammetry, wherein a decrease in a voltammetric peak as compared to a hybridizing control polynucleotide indicates a mutation of the capture in the target polynucleotide as compared to the control. -9-
Layer stacks claimed or described, ordered top of device to substrate.
chitosan-graphene modified glassy carbon electrode (CMG electrode)
Materials described outside the worked examples.
chitosan-graphene nanosheet material
chitosan
Performance values and ranges asserted in the specification or claims.
| Property | Value | Material |
|---|---|---|
sp2 vC-H stretching | 2920 cm⁻¹ | chitosan-graphene nanosheet material |
graphene
C
potassium ferrocyanide/ferricyanide redox probe
K4[Fe(CN)6]3-/4-
glassy carbon
sp2 vC-H stretching |
| 2849 cm⁻¹ |
chitosan-graphene nanosheet material |
vC=O | 1731 cm⁻¹ | chitosan-graphene nanosheet material |
vC=O | 1626 cm⁻¹ | chitosan-graphene nanosheet material |
skeletal vibrations of graphene sheets | 1481 cm⁻¹ | chitosan-graphene nanosheet material |
carboxyl vC-O stretching | 1461 cm⁻¹ | chitosan-graphene nanosheet material |
alkoxy vC-O stretching at edges of graphene | 1177 cm⁻¹ | chitosan-graphene nanosheet material |
vC-O stretching | 1051 cm⁻¹ | chitosan-graphene nanosheet material |
vCO-NH | 1635 cm⁻¹ | chitosan-graphene nanosheet material |
graphene
C
potassium ferrocyanide/ferricyanide redox probe
K4[Fe(CN)6]3-/4-
glassy carbon
sp2 vC-H stretching |
| 2849 cm⁻¹ |
chitosan-graphene nanosheet material |
vC=O | 1731 cm⁻¹ | chitosan-graphene nanosheet material |
vC=O | 1626 cm⁻¹ | chitosan-graphene nanosheet material |
skeletal vibrations of graphene sheets | 1481 cm⁻¹ | chitosan-graphene nanosheet material |
carboxyl vC-O stretching | 1461 cm⁻¹ | chitosan-graphene nanosheet material |
alkoxy vC-O stretching at edges of graphene | 1177 cm⁻¹ | chitosan-graphene nanosheet material |
vC-O stretching | 1051 cm⁻¹ | chitosan-graphene nanosheet material |
vCO-NH | 1635 cm⁻¹ | chitosan-graphene nanosheet material |
graphene
C
potassium ferrocyanide/ferricyanide redox probe
K4[Fe(CN)6]3-/4-
glassy carbon
sp2 vC-H stretching |
| 2849 cm⁻¹ |
chitosan-graphene nanosheet material |
vC=O | 1731 cm⁻¹ | chitosan-graphene nanosheet material |
vC=O | 1626 cm⁻¹ | chitosan-graphene nanosheet material |
skeletal vibrations of graphene sheets | 1481 cm⁻¹ | chitosan-graphene nanosheet material |
carboxyl vC-O stretching | 1461 cm⁻¹ | chitosan-graphene nanosheet material |
alkoxy vC-O stretching at edges of graphene | 1177 cm⁻¹ | chitosan-graphene nanosheet material |
vC-O stretching | 1051 cm⁻¹ | chitosan-graphene nanosheet material |
vCO-NH | 1635 cm⁻¹ | chitosan-graphene nanosheet material |
graphene
C
potassium ferrocyanide/ferricyanide redox probe
K4[Fe(CN)6]3-/4-
glassy carbon
sp2 vC-H stretching |
| 2849 cm⁻¹ |
chitosan-graphene nanosheet material |
vC=O | 1731 cm⁻¹ | chitosan-graphene nanosheet material |
vC=O | 1626 cm⁻¹ | chitosan-graphene nanosheet material |
skeletal vibrations of graphene sheets | 1481 cm⁻¹ | chitosan-graphene nanosheet material |
carboxyl vC-O stretching | 1461 cm⁻¹ | chitosan-graphene nanosheet material |
alkoxy vC-O stretching at edges of graphene | 1177 cm⁻¹ | chitosan-graphene nanosheet material |
vC-O stretching | 1051 cm⁻¹ | chitosan-graphene nanosheet material |
vCO-NH | 1635 cm⁻¹ | chitosan-graphene nanosheet material |